Therapeutic potential of morus nigra on 5-fluorouracil-induced gastrointestinal mucositis in rats

Year 2020, Volume: 42 Issue: 4, 363 - 372, 13.07.2020

Semra Yiğitaslan Ayşe Özkaraman Ayfer Açıkgöz Güler Balcı Alparslan Çiğdem Toprak Fatih Göger Erhan Sahin

https://doi.org/10.20515/otd.549371

Abstract

To investigate
the therapeutic potential of Morus Nigra
on the mucositis of digestive system induced via 5-fluorouracil in rats. A total
of 26 rats were divided into 4 groups as control (C), mucositis (M), Morus nigra extract-1(MN-1) and Morus nigra extract-2 (MN-2). Mucositis
was induced by intraperitoneal injection of 60 mg/kg 5-fluorouracil (5-FU). The
antioxidant enzyme levels in blood samples were analysed by ELISA. Histological
analysis and immunohistochemical staining for NF-kB and TNF-α were done. Increased
oxidative markers were found in mucositis group and increased antioxidative status
was found in MN-1 and MN-2 groups. All histochemical analyses suggested that
5-FU causes intestinal degeneration; the MN-1 extract reduced this degeneration
but did not completely treat it. The therapeutic potential of MN-2 extract was
lower than the MN-1. Results of the present study suggest that, though partly,
MN-1 extract in particular may have biochemically and histologically positive
effects against experimental mucositis induced by 5-FU in rats.

Keywords

Mucositis; Rat; Morus Nigra; 5-Fluorouracil

Morus Nigra'nın sıçanlarda 5-florourasil ile oluşturulmuş gastrointestinal mukozite karşı olası tedavi ediciliği

Abstract

Bu çalışmanın amacı sıçan gastrointestinal mukozasında 5-florourasil ile
oluşturulmuş mukozit üzerine Morus nigra ekstresinin olası tedavi edici
etkisini araştırmaktır.Toplam 26 adet sıçan eşit olarak kontrol, mukozit, mukozit+morus nigra ekstre 1
(MN-1) ve mukozit + morus nigra ekstre 2 (MN-2) olarak bölünmüştür. Mukozit sıçanlarda
60 mg/kg 5-fluorourasilin (5-FU) intraperitoneal olarak enjeksiyonuyla
oluşturulmuştur. ELISA ile kandaki oksidan ve antioksidan seviyeleri ölçüldü.
Histokimyasal ve immunohistokimyasal boyamalar (NF-kB ve TNF-α antikorları için)
yapıldı.  Mukozit grubunda artmış oksidatif stres belirteçleri görülürken Morus nigra ekstresi
verilen gruplarda antioksidan belirteçlerin arttığı saptandı. Histokimyasal
olarak 5-FU'nun mukozit oluşturduğu ve MN-1 ekstresinin tam olarak olmasa da
tedavi edici olduğu gösterildi. MN-1 ekstresi MN-2 ekstresine göre daha iyi bir
tedavi edici ajan olduğu belirlendi. Sonuç olarak bu çalışmada 5-FU ile oluşturulmuş mukozite karşı MN-1 ekstresinin
kısmi olarak tedavi edici olduğu biyokimyasal ve histopatolojik olarak gösterildi.

Keywords

Mukozit, Sıçan, Morus nigra, 5-florourasil

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