Böbrek Nakli Alıcılarında Cd4+ Ve Cd8+ T Lenfositlerin Vitamin D Reseptör İçerikleri ve Reseptör Ekspresyon Hızları

Year 2022, Volume: 44 Issue: 1, 25 - 36, 14.01.2022

Sultan Ural Edebali Sadi Köksoy Vural Taner Yılmaz Sebahat Özdem Fatih Yılmaz Hasan Sözel Fettah Fevzi Ersoy

https://doi.org/10.20515/otd.974905

Abstract

Bu çalışma, kronik allogreft disfonksiyonu (KAD) olan böbrek nakli (BN) alıcılarının CD4+ ve CD8+ lenfositlerinde D vitamini reseptörü (VDR) ekspresyonunu ve hücre içi VDR miktarlarını değerlendirmeyi amaçlamaktadır.Toplam 43 hasta (Grup 1:BN hastaları=29 hasta, böbrek biyopsisi ile KAD kanıtlanmış 15 hasta (Grup 1a), böbrek fonksiyonu stabil 14 hasta (Grup 1b), Grup 2:Kontrol grubu=14 sağlıklı birey) bu çalışmaya dahil edilmiştir. 25-hidroksikolekalsiferol, 1.25 dihidroksikolekalsiferol seviyeleri ölçüldü. Deneklerin CD4+ ve CD8+ tip T lenfositleri arasında VDR eksprese eden hücre sayısı, bu hücre gruplarının %'si olarak belirlendi. Hücre başına ortalama VDR molekülü içeriği ölçülmüş ve ortalama floresan yoğunlukları (MFI) olarak ifade edilmiştir.Grup 1 ve Grup 2 arasında 25-hidroksikolekalsiferol, 1.25 dihidroksikolekalsiferol düzeyleri ve CD4+ ve CD8+ hücrelerinde VDR eksprese eden hücrelerin yüzdeleri açısından fark bulunmadı (p>0.05). BN hastalarında CD4+/VDR(MFI) ve CD8+/VDR(MFI) değerleri sağlıklı kişilere göre daha yüksekti (p<0,001). BN hasta alt grupları karşılaştırıldığında, CD4+/VDR(%), CD8+/VDR(%), CD4+/VDR(MFI) ve CD8+/VDR(MFI) değerleri açısından istatistiksel olarak anlamlı fark yoktu (p>0.05).Bu çalışma, BN uygulanan hastaların T lenfositlerinde VDR'nin değişmediğini, ancak hücrelerdeki VDR içeriğinin serum 25-hidroksikolekalsiferol ve 1.25 dihidroksikolekalsiferol düzeylerinden bağımsız nedenlerle arttığını gösterdi.

Keywords

CD4+ lenfositler, CD8+ lenfositler, 25(OH)D3, 1.25(OH)2D3, Vitamin D reseptörü, Böbrek nakli

Supporting Institution

Çalışmamıza herhangi bir kurum desteği olmamıştır.

Vitamin D Receptor Contents and Receptor Expresslon Rates of Cd4+ and Cd8+ T Lymphocytes Ln Renal Transplant Reclplents

Abstract

This study aims to evaluate the vitamin D receptor (VDR) expression and intracellular amounts of VDRs in CD4+ and CD8+ lymphocytes of renal transplant (RT) recipients with chronic allograft dysfunction (CAD). A total of 43 patients (Group 1:RT patients=29 patients, 15 patients CAD proven by renal biopsy (Group 1a), 14 patients stable renal function (Group 1b), Group 2:Control group=14 healthy individuals) have been enrolled in this study. 25-hydroxycholecalciferol, 1.25 dihydroxycholecalciferol levels were measured. The number of cells expressing VDR among the CD4+ and CD8+ type T lymphocytes of the subjects was determined as % of those cell groups. The mean VDR molecule contents per cell have been measured and expressed as mean fluorescence intensities (MFI). No difference was found between Group 1 and Group 2 in terms of their 25-hydroxycholecalciferol, 1.25 dihydroxycholecalciferol levels, and the percentages of the cells expressing VDR in CD4+ and CD8+ cells (p>0.05). CD4+/VDR(MFI) and CD8+/VDR(MFI) values were higher in RT patients than healthy subjects (p<0.001). When the RT patient subgroups compared, there were no statistically significant differences regarding CD4+/VDR(%), CD8+/VDR(%), CD4+/VDR(MFI) and CD8+/VDR(MFI) values (p>0.05). This study showed VDR in T lymphocytes of patients who had RT did not change, but the VDR content in the cells increased due to reasons independent of serum 25-hydroxycholecalciferol and 1.25 dihydroxycholecalciferol levels.

Keywords

CD4+ lymphocytes, CD8+ lymphocytes, 25(OH)D3, 1.25(OH)2D3, Vitamin D receptor, Renal transplantation

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