Periferal Arter Hastalığında FBXW7, MASP1 ve SERP1 mRNA Ekspresyon Seviyelerinin İncelenmesi

Year 2026, Volume: 11 Issue: 1, 16 - 23, 15.03.2026

Bekir Erdoğan , Sibel Kuraş , Fatih Yıılmaz , Fatih Hacımustafaoğlu , Mahmud Esad Pençe , Halime Hanim Pence

https://doi.org/10.26453/otjhs.1812461

https://izlik.org/JA52WF29UH

Abstract

Amaç: Periferik Arter Hastalığı (PAD) dünya çapında kardiyovasküler morbiditenin üçüncü önde gelen nedenidir. Daha önce çalışılmamış olan “F-Box and WD domain containing 7” (FBXW7), “mannose-binding lectin-associated serine protease 1” (MASP1) ve “stress associated endoplasmic reticulum protein 1” (SERP1) genlerinin PAD hastalığındaki potansiyel rollerini araştırmayı amaçladık. Materyal ve Metot: Toplam 78 periferal arter hastalığı olan birey ve 76 sağlıklı birey çalışmaya dahil edilmiştir. Total RNA kan örneklerinden izole edildi ve FBXW7, MASP1 ve SERP1 mRNA ekspresyonları kantitatif gerçek zamanlı polimeraz zincir reaksiyonu kullanılarak belirlenmiştir. Bulgular: FBXW7, MASP1 ve SERP1 genlerinin ifade düzeyleri periferik arter hastalığı olan hastalar ile sağlıklı bireyler arasında istatistiksel olarak farklılık göstermemiştir (p>0,05). Sonuç: FBXW7, MASP1 ve SERP1 proteinlerinin arterle ilişkisi olmasına rağmen periferik arter hastalığının gelişiminde etkili değildirler.

Keywords

FBXW7 geni , MASP1 geni , mRNA ekspresyonu , Periferik arter hastalığı , SERP1 geni

Ethical Statement

Çalışma için etik onay, XXX Yüksek Uzmanlık Eğitim ve Araştırma Hastanesi Klinik Araştırma Etik Kurulu'ndan alınmıştır (Tarih: 20.12.2018, karar no: 2018/7/72). Çalışma, Helsinki Bildirgesi'ne uygun olarak yürütülmüştür.

Supporting Institution

Proje, Türkiye Sağlık Bilimleri Üniversitesi, Bilimsel Araştırma Projeleri Birimi tarafından desteklenmiştir (proje no: 2019/035).

Investigation of FBXW7, MASP1 and SERP1 mRNA Expression Levels in Patients with Peripheral Artery Disease

https://izlik.org/JA52WF29UH

Abstract

Objective: Peripheral Artery Disease (PAD) is the third leading cause of cardiovascular morbidity worldwide. Given their established roles in lipid metabolism, the lectin-complement pathway, and endoplasmic reticulum stress, we aimed to investigate the potential involvement of the FBXW7, MASP1, and SERP1 genes in PAD. This study sought to evaluate whether these genes could serve as non-invasive biomarkers for the diagnosis of PAD, as they have not been previously explored in this patient population. Materials and Methods: A total of 78 individuals with peripheral arterial disease and 76 healthy individuals were included in the study. Total RNA was extracted from blood samples, and the expressions of FBXW7, MASP1, and SERP1 mRNAs were determined using the real-time polymerase chain reaction (rt-PCR). Results: No statistically significant differences were observed in the expression levels of FBXW7, MASP1, and SERP1 genes between PAD patients and healthy subjects (p > 0.05). Conclusion: Although FBXW7, MASP1, and SERP1 proteins are associated with arterial function, they do not appear to play a direct role in the development of peripheral artery disease.

Keywords

FBXW7 gene , MASP1 gene , mRNA expression , Peripheral artery disease , SERP1 gene

Ethical Statement

Ethical approval for the study was obtained from the XXX High Specialization Education and Research Hospital, Clinical Research Ethics Committee (Date: 20.12.2018, decision no: 2018/7/72). The study was conducted in accordance with the Declaration of Helsinki.

Supporting Institution

The project was supported by the University of Health Sciences Türkiye, Unit of Scientific Research Projects (project no: 2019/035).

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