Periferal Arter Hastalığında FBXW7, MASP1 ve SERP1 mRNA Ekspresyon Seviyelerinin İncelenmesi

Öz

Amaç: Periferik Arter Hastalığı (PAD) dünya çapında kardiyovasküler morbiditenin üçüncü önde gelen nedenidir. Daha önce çalışılmamış olan “F-Box and WD domain containing 7” (FBXW7), “mannose-binding lectin-associated serine protease 1” (MASP1) ve “stress associated endoplasmic reticulum protein 1” (SERP1) genlerinin PAD hastalığındaki potansiyel rollerini araştırmayı amaçladık. Materyal ve Metot: Toplam 78 periferal arter hastalığı olan birey ve 76 sağlıklı birey çalışmaya dahil edilmiştir. Total RNA kan örneklerinden izole edildi ve FBXW7, MASP1 ve SERP1 mRNA ekspresyonları kantitatif gerçek zamanlı polimeraz zincir reaksiyonu kullanılarak belirlenmiştir. Bulgular: FBXW7, MASP1 ve SERP1 genlerinin ifade düzeyleri periferik arter hastalığı olan hastalar ile sağlıklı bireyler arasında istatistiksel olarak farklılık göstermemiştir (p>0,05). Sonuç: FBXW7, MASP1 ve SERP1 proteinlerinin arterle ilişkisi olmasına rağmen periferik arter hastalığının gelişiminde etkili değildirler.

Anahtar Kelimeler

• FBXW7 geni
• MASP1 geni
• mRNA ekspresyonu
• Periferik arter hastalığı
• SERP1 geni

Investigation of FBXW7, MASP1 and SERP1 mRNA Expression Levels in Patients with Peripheral Artery Disease

Öz

Objective: Peripheral Artery Disease (PAD) is the third leading cause of cardiovascular morbidity worldwide. Given their established roles in lipid metabolism, the lectin-complement pathway, and endoplasmic reticulum stress, we aimed to investigate the potential involvement of the FBXW7, MASP1, and SERP1 genes in PAD. This study sought to evaluate whether these genes could serve as non-invasive biomarkers for the diagnosis of PAD, as they have not been previously explored in this patient population. Materials and Methods: A total of 78 individuals with peripheral arterial disease and 76 healthy individuals were included in the study. Total RNA was extracted from blood samples, and the expressions of FBXW7, MASP1, and SERP1 mRNAs were determined using the real-time polymerase chain reaction (rt-PCR). Results: No statistically significant differences were observed in the expression levels of FBXW7, MASP1, and SERP1 genes between PAD patients and healthy subjects (p > 0.05). Conclusion: Although FBXW7, MASP1, and SERP1 proteins are associated with arterial function, they do not appear to play a direct role in the development of peripheral artery disease.

Anahtar Kelimeler

• FBXW7 gene
• MASP1 gene
• mRNA expression
• Peripheral artery disease
• SERP1 gene

Kaynakça

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