Correlation of E-Cadherin/Beta Catenin Expression with Localization in Squamous Epithelial Cell Carcinoma and Basal Cell Carcinoma

Abstract

Objective: The aim of this study is to determine whether E-cadherin and β-catenin levels in cases with squamous epithelial cell cancer observed in certain regions of the body vary according to the patient’s age, gender, tumor localization and tumor type. Materials and Methods: The sample of this retrospective study was the samples of 53 patients who applied to Ordu University Training and Research Hospital and who were diagnosed with flat epithelial cell tumors on the skin. in the sample; It was evaluated immunohistochemically in terms of β –catenin and E-cadherin. Results: There was no difference between the age distribution of the patients (p> 0.05). β-catenin and Ecadherin scores; It does not change according to the gender and the location of the sample (p= 0.129, 0.106 and 0.259, 0.120, respectively). According to the tumor type, it was determined that β-catenin scores did not change but E -cadherin changed significantly (p =0.721, 0.013, respectively). Conclusions: According to the tumor type, it was determined that E-cadherin scores changed significantly. Scientific studies to be carried out by enlarging the sample width will contribute significantly to the result.

Keywords

• Basal cell
• β –catenin
• E- kadherin
• squamaus epithelium

Yassı Epitel Hücreli Karsinom ve Bazal Hücreli Karsinomlarda E-Kadherin, β- Katenin Ekspresyonunun Lokalizasyonla İlişkisi

Öz

Amaç: Bu çalışmanın amacı; vücudun bazı bölgelerinde görülen yassı epitel hücreli karsinom vakalarında Ekadherin ve β-katenin düzeylerinin; hastaların yaşı, cinsiyeti, karsinomların yerleşim yeri ve tümör tipine göre değişip değişmediğini ortaya koymaktır. Materyal ve Metot: Retrospektif olarak yapılan bu çalışmanın örneklemini Ordu Üniversitesi Eğitim Araştırma hastanesine müracaat eden ve deride yassı epitel hücreli karsinom tanısı almış olan 53 hastanın numuneleri oluşturdu. Numuneler E-kadherin ve β-katenin yönünden immünohistokimyasal olarak değerlendirildi. Bulgular: Hastaların yaş dağılımları arasında fark tespit edilmedi (p>0,05). β-katenin ve E- kadherin skorları; cinsiyet ve numunenin alındığı yerleşim yerine göre değişmemektedir (p değerleri sırasıyla; 0,129, 0,106 ve 0,259, 0,120). Tümör tipine göre ise β-katenin skorlarının değişmediği fakat E- kadherin skorlarının önemli oranda değiştiği belirlendi (sırasıyla p değeri; 0,721, 0,013). Sonuç: E-kadherin boyama şiddetinin patolojik tanı tipine göre değiştiği belirlendi. Örneklem genişliği büyütülerek yapılacak bilimsel çalışmalar sonuca önemli katkı sağlayacaktır.

Anahtar Kelimeler

• Bazal hücre
• β-katenin
• E- kadherin
• yassı epitel

References

1. Dursun E, Korkmaz H, Bayız Ü, et al. Nonmelanoma skin cancers of the head and neck region. KBB ve BBC Dergisi. 2002;10(3):156–163.
2. Ahmad I, Gupta A. Epidemiology of basal cell carcinoma and squamous cell carcinoma of the pinna. J Laryngol Otol. 2001;115:85-86.
3. Cenık Z, Uyar Y, Doğmuş H. Baş ve boyun bölgesi malign deri tümörleri. Türk ORL Arşivi. 1992;30:56-59.
4. Flemıng ID, Amonette R, Monaghan T, Flemıng MD. Principles of management of basal and squamous cell carcinoma of the skin. Cancer. 1995;75:699-704.
5. Palaghıa M, Mıhaı C, Lozneanu L, et al. Ecadherin expression in primary colorectal cancer and metastatic lymph nodes. Rom J Morphol Embryol. 2016;57(1):205–209.
6. Gumbiner BM: Cell adhesion: The molecular basis of tissue architecture and morphogenesis. Cell. 1996;84:345-357.
7. Barth AI, Nathke IS, Nelson WJ. Cadherins, catenins and APC protein: interplay between cytoskeletal complexes and signaling pathways. Curr Opin Cell Biol.1997;9:683-690.
8. Gallin WJ, Sorkin BC, Edelman GM, Cunningham BA. Sequence analysis of a cDNA clone encoding the liver cell adhesion molecule, LCAM. Proc Natl Acad Sci USA. 1987;84:2808- 2812.

9. Tekeşin M, Eraslan N, Aksakallı N, et al. Oral Squamous cell carsinoma: Retrospective study of 147 cases. GU. Dental Medicine Journal. 2012;29 (2):93-98.
10. Semb H, Christofori G. The tumor-suppressor function of E-cadherin. Am J Hum Gene. 1998;63:1588-1593.
11. Murata-Kamiya N, Kurashima Y, Teishikata Y, et al. Helicobacter pylori CagA interacts with Ecadherin and deregulates the β-catenin sig-nal that promotes intestinal transdifferentia-tion in gastric epithelial cells. Oncogene. 2007;26:4617-4626.
12. Tanır HG., Demirezen S., Beksaç MS., Biomolecules of target cell membrane involved in the Wnt/Beta-catenin signaling pathway. Marmara Medical Journal.2012;25:53-57.
13. Kikuchi A, Yamamoto H. Tumor formation due to abnormalities in the beta-catenin-independent pathway of Wnt signalling. Cancer Sci. 2008;99:202-208.
14. Lustig B, Behrens J. The Wnt signaling pathway and its role in tumor development. J Cancer Res Clin Oncol. 2003;129:199-221.
15. Yuan S., Tao F., Zhang X., Zhang Y., Sun X., Wu D. Role of Wnt/β-Catenin signaling in the chemoresistance modulation of colorectal cancer. Hindawi BioMed Research International.2020; 2020:1-9.
16. Yuwanati MB., Tupkari JV., Avadhani A. Expression of E-cadherin in oral epithelial dysplasia and oral squamous cell carcinoma: An in vivo study. Journal of Clinical and Experimental Investigations.2011; 2(4): 347-353.
17. Pan X, Ma L, Wang J. The clinicopathological significance and prognostic value of β-catenin Ser45-phosphorylation expression in esophageal squamous cell carcinoma. Int J Clin Exp Pathol. 2019;12(9):3507-3513.
18. Alpar R. Uygulamalı Çok Değişkenli İstatistiksel Yöntemler. 3.Baskı, Ankara, Detay Yayıncılık, 2011.
19. Ress DG. Foundations of Statistics; Chapman and Hall, New York, London Press. 1987.
20. Petrovaa YI., Schectersonb L., Gumbinerb BM. Roles for E-cadherin cell surface regulation in cancer. Molecular Biology of the Cell.2016;27 (21):3233-3244.
21. Mendonsa AM., Na TY., Gumbiner BM. Ecadherin in contact inhibition and cancer. Oncogene.2018;37:4769-4780.
22. Bruner HC., and Derksen PWB. Loss of Ecadherin-dependent cell–cell adhesion and the development and progression of cancer. Cold Spring Harb Perspect Biol. 2018; 10(3): 1-20.
23. Veeman MT, Axelrod JD, Moon RT. A second canon: functions and mechanisms of β-cateninindependent Wnt signaling. Dev Cell. 2003;5:367 -377.
24. Chaw SY, Abdul Majeed A, Dalley AJ, et al. Epithelial to mesenchymal transition (EMT) biomarkers – E-cadherin, beta-catenin, APC and Vimentin – in oral squamous cell carcinogenesis and transformation. Oral Oncology. 2012;48:997– 1006.
25. Umbreı C, Erben P, Faber A, et al. Lapatinib induced mesenchymal epithelial transition in squamous cell carcinoma cells correlates with unexpected alteration of β catenin expression. Oncology Letters. 2016;11:2715-2724.
26. Tharmalingam N, Park M, Lee MH, et al. Tumor suppressor gene E-cadherin and its role in normal and malignant cells. Cancer Cell International. 2003;3:17.
27. Alyamaç M, Bozat AD, Güler F, Yıldız D. Bazal hücreli karsinom tedavisinde nüks oranları. Başkent Üniversitesi Tıp Fakültesi dergisi. 2016;3: (1);7-13.
28. Aktürk A, Yıldız KD, Bilen N, et al. Kocaeli Üniversitesi Tıp Fakültesine 1996-2003 yılları arasın da başvuran deri karsinomları olguları. Türkiye Klinikleri J Dermatol. 2006;16:44-49.
29. Baş Y, Kalkan G, Pancar GS, Yıldız H, Müslehiddinoğlu A. Skin cancer cases admitted to Tokat State Hospital between years 2007 and 2011. Turk J Dermatol. 2014;2:84-87.
30. Pereira CH, Morais MO, Martins AF, et al. Expression of adhesion proteins (E-cadherin and β-catenin) and cell proliferation (Ki-67) at the invasive tumor front in conventional oral squamous cell and basaloid squamous cell carcinomas. Arch Oral Biol. 2016;61:8-15.