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Characterization of polyphenol oxidase from red deadnettle (lamium purpureum) and its inhibition

Year 2016, , 245 - 250, 01.08.2016
https://doi.org/10.16984/saufenbilder.80440

Abstract

In this work, it is investigated the kinetic properties of polyphenol oxidase (PPO) obtained from Lamium purpureum (red deadnettle).  4-methylcatechol was used as substrate in the characterization studies. Michaelis-Menten constant (KM) and maximum reaction velocity (Vmax) were calculated. Enzyme’s optimum pH value was found 7,5; optimum temperature value was found 10 . KM and Vmax values were calculated 2,9977 mM and 0,0087 EU/min respectively. PPO activity substantially was inhibited from sodium azide, thiourea, L-cysteine, ascorbic acid, benzoic acid, citric acid, 2-mercaptoethanol and I50 values were calculated each inhibitor.

Eflatun çiçekli ballıbaba (lamium purpureum) polifenol oksidaz enziminin karakterizasyonu ve inhibisyonu

Year 2016, , 245 - 250, 01.08.2016
https://doi.org/10.16984/saufenbilder.80440

Abstract

Bu çalışmada, Lamium purpureum (eflatun çiçekli ballıbaba) bitkisinden elde edilen polifenol oksidaz (PPO) enziminin kinetik özellikleri incelenmiştir. Karakterizasyon çalışmalarında substrat olarak 4-metil katekol kullanılmıştır. 4-metil katekol için Michaelis-Menten sabiti (KM) ve maksimum reaksiyon hızı (Vmax) hesaplanmıştır. Enziminin optimum pH değeri 7,5, optimum sıcaklık değerleri ise 10 °C bulunmuştur. Vmax ve KM değerleri ise sırasıyla 2,9977 mM ve 0,0087 EU/dak olarak hesaplanmıştır. PPO enzimi için sodyum azid, tiyoüre, L-Sistein, askorbik asit, sitrik asit, benzoik asit, 2-merkaptoetanol ile inhibisyon çalışması yapılmış, her bir inhibitör için I50 değeri hesaplanmıştır.

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Details

Subjects Engineering
Journal Section Research Articles
Authors

Elif Cerrahoğlu

Gülnur Arabacı This is me

Publication Date August 1, 2016
Submission Date April 25, 2014
Acceptance Date February 3, 2016
Published in Issue Year 2016

Cite

APA Cerrahoğlu, E., & Arabacı, G. (2016). Characterization of polyphenol oxidase from red deadnettle (lamium purpureum) and its inhibition. Sakarya University Journal of Science, 20(2), 245-250. https://doi.org/10.16984/saufenbilder.80440
AMA Cerrahoğlu E, Arabacı G. Characterization of polyphenol oxidase from red deadnettle (lamium purpureum) and its inhibition. SAUJS. August 2016;20(2):245-250. doi:10.16984/saufenbilder.80440
Chicago Cerrahoğlu, Elif, and Gülnur Arabacı. “Characterization of Polyphenol Oxidase from Red Deadnettle (lamium Purpureum) and Its Inhibition”. Sakarya University Journal of Science 20, no. 2 (August 2016): 245-50. https://doi.org/10.16984/saufenbilder.80440.
EndNote Cerrahoğlu E, Arabacı G (August 1, 2016) Characterization of polyphenol oxidase from red deadnettle (lamium purpureum) and its inhibition. Sakarya University Journal of Science 20 2 245–250.
IEEE E. Cerrahoğlu and G. Arabacı, “Characterization of polyphenol oxidase from red deadnettle (lamium purpureum) and its inhibition”, SAUJS, vol. 20, no. 2, pp. 245–250, 2016, doi: 10.16984/saufenbilder.80440.
ISNAD Cerrahoğlu, Elif - Arabacı, Gülnur. “Characterization of Polyphenol Oxidase from Red Deadnettle (lamium Purpureum) and Its Inhibition”. Sakarya University Journal of Science 20/2 (August 2016), 245-250. https://doi.org/10.16984/saufenbilder.80440.
JAMA Cerrahoğlu E, Arabacı G. Characterization of polyphenol oxidase from red deadnettle (lamium purpureum) and its inhibition. SAUJS. 2016;20:245–250.
MLA Cerrahoğlu, Elif and Gülnur Arabacı. “Characterization of Polyphenol Oxidase from Red Deadnettle (lamium Purpureum) and Its Inhibition”. Sakarya University Journal of Science, vol. 20, no. 2, 2016, pp. 245-50, doi:10.16984/saufenbilder.80440.
Vancouver Cerrahoğlu E, Arabacı G. Characterization of polyphenol oxidase from red deadnettle (lamium purpureum) and its inhibition. SAUJS. 2016;20(2):245-50.

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