Drosophila Melanogaster'in Biyolojik özelliklerine nanofiberin etkisi

Year 2017, Volume: 21 Issue: 6, 1608 - 1612, 01.12.2017

Eda Güneş , Mehmet Okan Erdal , Lokman Gemi

https://doi.org/10.16984/saufenbilder.321646

Abstract

Günümüzde nanofiber komponenler (poliakrilonitril dimetil formamit gibi)
bir çok mühendislik alanında ve gıda sektöründe kullanılmaktadır. Çevre ve
hedef olmayan organizmalar üzerindeki muhtemel etkilerin belirlenmesi
gerekmektedir.  Drosophila melanogaster
Meigen (Diptera: Drosophilidae) gelişim biyolojisi ve çevre çalışmalarında çok
çalışılan model bir organizmadır. Bu çalışmada, erginler nanofiber
(elektrospinle üretilen 260 nm çap aralığına sahip PAN-DMF) kaplı diyetle
beslenerek yetiştirilmiş, ergin evreye kadar biyolojik özellikler gözlenmiştir.
Nano fiberin etkisi ile D. melanogaster'in  yaşama gelişme ve hayatta
kalma oranları araştırılmıştır. Besin yüzeyi ve canlılık SEM kullanarak
görüntülenmiştir. Elde edilen sonuçlara göre, PAN-DMF kontrolle
karşılaştırıldığında besin yüzeyinin uygun olduğu görülmüş; ve böceğin gelişim
sürecini kötü etkilemediği bulunmuştur. Besinle ilişkili organizmalarda
nanofiberler kullanılabilir, fakat bu bileşenlerin çevre ve hedef olmayan
organizmalar üzerindeki etkilerinin anlaşılabilmesi için çok detaylı
çalışmalara gerek duyulmaktadır.

Keywords

Nanofiber, Drosophila melanogaster, Beslenme, Gelişim

The effect of nanofiber on the biological traits of Drosophila Melanogaster

Abstract

Today nanofiber components (polyacrylonitrile dimethyl
formamide etc.) are used in many engineering fields and food industry. Possible
effects are need to be identified on the environment and non-target organisms. Drosophila melanogaster Meigen (Diptera:
Drosophilidae) has
been one of the most studied organisms, working as a model in developmental
biology and environmental studies. In this study, adults were fed with
nanofiber coated diet (PAN-DMF; produced by the electrospinning, range of
diamer 260 nm), eggs were obtained from adults, and biological traits were
observed until the adult stage. The effect of nanofiber on survivorship and
development ratio of  D.
melanogaster were investigated. Nutrient surface and viability were
visualized by using SEM. According to the obtained results, PAN-DMF is suited
with the nutrient surface compared to controls; and was found to have no
adverse effect on the development of insect. Nanofibers can be used in food
contact organisms, but more detailed studies would be needed to understand the
effects of these components on environment and non target organisms.

Keywords

Nanofiber, Drosophila melanogaster, Nutrition, Development

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