Optimization of exopolysaccharide production of Lactobacillus brevis E25 using RSM and characterization

Year 2020, Volume: 24 Issue: 1, 151 - 160, 01.02.2020

Ertan Ermiş Ecem Poyraz Enes Dertli Mustafa Tahsin Yılmaz

https://doi.org/10.16984/saufenbilder.545929

Cited By: 4

Abstract

Response surface
methodology was used to determine the exopolysaccharide production of Lactobacillus brevis E25. The effects of three variables,
temperature (30, 36 and 42 °C), incubation time (18, 33 and 48 h) and initial
pH of growth medium (4.5, 5.5 and 6.5) were studied. Under optimum growth conditions, the amount of exopolysaccharide
derived from Lactobacillus brevis E25 ranged from 10 to 35 gL^-1. The size of EPS molecules ranged from 10⁵ to 10⁷ Da.
Infra red spectrum analysis
showed characteristics absorption peaks indicating the presence of -OH, C=O and
C-H groups. Furthermore, only glucose was detected as monosaccharide in
exopolysaccharide structure, revealing that the structure of exopolysaccharide
is a homopolymeric glucan type. Based on the differential scanning calorimeter
thermograms, exopolysaccharide’s melting temperature was observed around 116 °C.

Keywords

Lactic acid bacteria, exopolysaccharide, response surface methodology, molecular characterization

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