The Evaluations Of Taxonomic Classifications In The Genus Trifolium L. Based On ITS Sequences

Year 2022, Volume: 26 Issue: 3, 545 - 553, 30.06.2022

Aykut Yılmaz Yudum Yeltekin

https://doi.org/10.16984/saufenbilder.1074625

Abstract

The genus Trifolium L. belonging to family Fabaceae is represented by about 255 species in the world. Two classifications based on the sections or subgenus are commonly used by the researchers in the taxonomically evaluation of the genus Trifolium. However, there are still some confusing and complex situations about the taxonomy of the genus. For this reason, internal transcribed spacer (ITS) regions between rDNA genes in nrDNA were examined for 139 Trifolium taxa with the Maximum Parsimony (MP) method. Furthermore MP tree were evaluated based on two classification used commonly in the genus to provide contributions to taxonomic classification of the Trifolium species and to evaluate phylogenetic relationships of the Trifolium species. Study results support the status of subgenus proposed by new infrageneric classification. Also it can be stated that it is rationale to divide the section Lotoidea into several sections according to phylogenetic relationships of the taxa examined. Finally, this study reveals the importance of sequence information and problems in the barcoding.

Keywords

Trifolium, Fabaceae, Internal transcribed spacer, Maximum parsimony, Lotoidea

Thanks

The authors would like to thank to NCBI for sequence informations used in this study and the authors sharing the sequence informations in NCBI.

References

• [1] LPWG, Legume Phylogeny Working Group, “A new subfamily classification of the Leguminosae based on a taxonomically comprehensive phylogeny,” Taxon, vol. 66, no. 1, pp. 44–77, 2017.
• [2] I. Choi, T. A. Ruhlman, and R. K. Jansen, “Comparative Mitogenome Analysis of the Genus Trifolium Reveals Independent Gene Fission of ccmFn and Intracellular Gene Transfers in Fabaceae,” International Journal of Molecular Sciences, vol. 21, no. 6, pp. 1959, 2020.
• [3] E. Uslu and M. T. Babaç, “A descriptive study of some Trifolium L. (Clover) taxa grown in Bolu Province,” Turkish Journal of Botany, vol. 43, pp. 673–686, 2019.
• [4] V. A. Kalinkina, V. M. Yulia, and E. K. Dmitry, “Diversity and taxonomy of the Trifolium lupinaster polymorphic complex in Eastern Europe and Asia,” Flora, vol. 267, pp. 151597, 2020.
• [5] N. W. Ellison, A. Liston, J. J. Steiner, W. M. Williams, and N. L. Taylor, “Molecular phylogenetics of the clover genus (Trifolium—Leguminosae),” Molecular Phylogenetics and Evolution, vol. 39, no. 3, pp. 688–705, 2006.
• [6] M. Zohary and D. Heller, “The Genus Trifolium,” The Israel Academy of Science and Humanities, Jerusalem, 1984.
• [7] E. Uslu, G. D. Ertuğrul, and M.T. Babaç, “Assessment of genetic diversity in naturally growing 29 Trifolium L. taxa from Bolu Province using RAPD and SSR markers,” Turkish Journal of Biology, vol. 37, pp. 479–490, 2013.
• [8] L. E. Watson, H. Sayed-Ahmed, and A. Badr, “Molecular phylogeny of Old World Trifolium (Fabaceae), based on plastid and nuclear markers,” Plant Systematics and Evolution, vol. 224, pp. 153–171, 2000.
• [9] NCBI, National Centre of Biotechnology Information,https://www.ncbi.nlm.nih.gov/genbank.
• [10] M.J. Sanderson and M. F. Wojciechowski, “Diversification rates in a temperate legume clade: Are there ‘‘so many species’’ of Astragalus (Fabaceae)?,” American Journal of Botany, vol. 83, no. 11, pp. 1488–1502, 1996.
• [11] J. Steiner, E. Piccioni, M. Falcinelli, and A. Liston, “Germplasm Diversity among Cultivars and the NPGS Crimson Clover Collection,” Crop Science, vol. 38, no. 1, pp. 263–271, 1998.
• [12] H. Schaefer, P. Hechenleitner, A. Santos-Guerra, M. M. de Sequeira, R. T. Pennington, G. Kenicer, and M. A. Carine, “Systematics, biogeography, and character evolution of the legume tribe Fabeae with special focus on the middle-Atlantic island lineages,” BMC Evolutionary Biology, vol. 12, no. 1, pp. 250, 2012.
• [13] L. Vizintin and B. Bohanec, “Measurement of nuclear DNA content of the genus Trifolium L. as a measure of genebank accession identity,” Genetic Resources and Crop Evolution, vol. 55, pp. 1323–1334, 2008.
• [14] A. H. Thornhill, B. G. Baldwin, W. A. Freyman, S. Nosratinia, M. M. Kling, N. Morueta-Holme, T. P. Madsen, D. D. Ackerly and B. D. Mishler, “Spatial phylogenetics of the native California flora,” BMC Biology, vol. 15, no. 1, pp. 96, 2017.
• [15] S. Z. Xu, Z.Y. Li, and X. H. Jin, “DNA barcoding of invasive plants in China: a resource for identifying invasive plants,” Molecular Ecology Resources, vol. 18, no. 1, pp. 128–136, 2017.
• [16] Q. R. Bai, X. Y. Jiang, Y. Y. Xie, H. Y. Sun, and J. Gao, “Summer Blight of White Clover (Trifolium repens) Caused by Rhizoctonia solani AG-1-IB in China,” Plant Disease, vol. 98, no. 8, pp. 1153, 2014.
• [17] S. Kumar, G. Stecher, M. Li, C. Knyaz, and K. Tamura, “MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms,” Molecular Biology and Evolution, vol. 35, pp. 1547–1549, 2018.
• [18] J. Felsenstein, “Confidence limits on phylogenies: An approach using the bootstrap,” Evolution, vol. 39, pp. 783–791, 1985.
• [19] A. Badr, “Electrophoretic studies of seed proteins in relation to chromosomal criteria and the relationships of some taxa of Trifolium,” Taxon, vol. 44, pp. 183–191, 1995.
• [20] L. Vizintin, B. Javornik, and B. Bohanec, “Genetic characterization of selected Trifolium species as revealed by nuclear DNA content and ITS rDNA region analysis,” Plant Science, vol. 170, no. 4, pp. 859–866, 2006.