Comparision of the Bactericidal Effect of the UV and Blue-Light Regions on Selected Escherichia Coli and Staphylococcus Aureus Strains

Year 2024, Volume: 28 Issue: 5, 937 - 944, 25.10.2024

Can Saç

Abstract

Some Bacteria are important microorganisms that threaten human health. Especially Escherichia coli and Staphylococcus aureus can cause serious diseases in humans. Antibiotics are used to stop these infections and prevent bacteria from multiplying. However, it has been observed that these antibiotics have side effects as well as their benefits. Therefore, the resistance of these bacteria can be reduced with rays of different wavelengths. In our study, the effects of three types of light with wavelengths of 254 nm, 365 nm and 460 nm on Escherichia coli and Staphylococcus aureus bacteria were investigated. The reason we chose these rays is that two of them have short wavelengths and are harmful rays, while the other one is in the visible region and is harmless. While a significant decrease in the number of colonies was observed under the operating conditions of 254 nm and 365 nm wavelength lights falling in the UV region, no colonies were observed in the 460 nm wavelength light. In the second trial results, 44% growth was achieved at 365 nm and 56% growth was inhibited. S. aureus growth stopped completely at 460 nm. At 254 nm, 14% growth was achieved and 86% growth was inhibited. It was observed that there was 3% growth and 97% growth inhibition at 365 nm. As can be clearly seen from the results, no significant bacterial growth was observed at 460 nm. In our literature studies, it can be seen that no study has been done on this wavelength before.

Keywords

Echerichia coli, Staphylococcus aureus, Blue light

References

• S. Singha, R. Thomas,J. N. Viswakarma, V. K. Gupta, “Foodborne illnesses of Escherichia coli O157origin and its control measures” Journal of Food Science and Technology, Vol. 60, no. 4), pp. 1274-1283, 2023.
• Y. Wang, K. Fu, “Genotoxins: The Mechanistic Links between Escherichia coli and Colorectal Cancer” Cancers, vol. 15(, no. 4, p. 1152, 2023.
• C. dos Anjos, L. G. Leanse, M. S. Ribeiro, F. P. Sellera, M. Dropa, V. E. Arana-Chavez, N. Lincopan, M. S. Baptista, F. C. Pogliani, T. Dai, C. P. Sabino, “New Insights into the Bacterial Targets of Antimicrobial Blue Light” Microbiology Spectrum, Vol.11, no. 2, 2023.
• N. Özkütük, Use of Ultraviolet Lamps, 5th National Sterilization Disinfection Congress, Antalya, Turkey, 2007.
• T. Dai, A. Grupta, Y. Huang, M.E. Sherwood, C.K. Murray, T. Kiellan, and M. R. Hamblin, ‘’Blue Light Eliminates Community-Acquired Methicillin-Resistant Staphylococcus aureus in Infected Mouse Skin Abrasions’’, Photomedicine and Laser Surgery, Vol.31, p.11, 2013.
• R. Lubart, A. Lipovski, Y. Nitzan, And H. A. Friedmann, ‘’Possible Mechanism for The Bactericidal Effect of Visible Light’’, Laser Therapy, vol. 20, no. 1, pp. 17-22, 2011.
• M. Arda, Fundamentals of Microbiology, Second Expanded Edition. Medisan Publication Series No 46, Ankara, 2000. M. Clauß, ‘’Higher effectiveness of photoinactivation of bacterial spores, UV resistant vegetative bacteria and mold spores with 222 nm compared to 254 nm wavelength’’ Acta Hydrochimica et Hydrobiologica, vol. 34, pp. 525-532, 2006.
• V.V. Bumah, D.S. Masson-Meyers, S.E. Cashin, C.S. Enwemeka, ‘’Wavelength and bacterial density influence the bactericidal effect of blue light on methicillin-resistant Staphylococcus aureus (MRSA)’’, Photomedicine and Laser Surgery, vol. 31, no. 11, pp. 547-553, 2013.
• YQ. Li, Q. Han, JL. Feng, WL. Tian, HZ. Mo,“Antibacterial characteristics and mechanisms of 3-poly-lysine against Escherichia coli and Staphylococcus aureus”, Food Control, vol. 43, pp. 22-27, 2014.
• Y. Zhang, X. Liu, Y. Wang, P. Jiang, SY. Quek, “Antibacterial activity and mechanism of cinnamon essential oil against Escherichia coli and Staphylococcus aureus”, Food Control, vol. 59, pp. 282-289, 2016.
• D.A. Phoenix, Z. Sayed, S. Hussain, F. Harris, M. Wainwright, “The phototoxicity of phenothiazinium derivatives against Escherichia coli and Staphylococcus aureus”, FEMS Immunology and Medical Microbiology, vol. 39, pp. 17-22, 2003.
• C.L. Fischer, K.S. Walters, D.R. Drake, D.R. Blanchette, D.V. Dawson, K.A. Brogden, P.W. Wertz, “Sphingoid Bases Are Taken Up by Escherichia coli and Staphylococcus aureus and Induce Ultrastructural Damage”, Skin Pharmacol Physiol, vol. 26, pp. 36–44, 2013.
• K.J. Williams, L. J. Piddock, “Accumulation of rifampicin by Escherichia coli and Staphylococcus aureus”, Journal of Antimicrobial Chemotherapy, vol. 42, pp. 597-603, 1998.
• J. C. Lopez-Romero, H. González-Ríos, A. Borges, M. Simões, “Antibacterial Effects and Mode of Action of Selected Essential Oils Components against Escherichia coli and Staphylococcus aureus”, Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine, vol. 2015, pp. 9, 2015.
• N. Turgut, “Investigation of the Presence of E.Coli O157-H7 in Lettuce Served for Consumption in Aydın Province, Adnan Menderes University Health Sciences Institute, Department of Microbiology, Doctoral Thesis, 2015.
• D. Douglas Bannerman, Max J. Paape, Jai-Wei Lee, Xin Zhao, Jayne C. Hope, Pascal Rainard, “Escherichia coli and Staphylococcus aureus Elicit Differential Innate Immune Responses following Intramammary Infection”, Clinical and Diagnostic Laboratory Immunology, vol. 11, no. 3, pp. 463-472, 2004
• F. Blanche, B. Cameron, F. X. Bernard, L. Maton, B. Manse, L. Ferrero, N. Ratet, C. Lecqo, A. Goniot, D. Bisch, J. Crouzet, “Differential Behaviors of Staphylococcus aureus and Escherichia coli Type II DNA Topoisomerases”, Antimicrobial Agents and Chemotherapy, vol. 40, no. 12, 2714-2720 pp. 2714–2720, 1996.
• Q. L. Feng, J. Wu, G. Q. Chen, F. Z. Cui, T. N. Kim, J. O. Kim, “A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus”, Journal of biomedical materials research, vol. 52, no. 4, pp. 662-668. 2000.
• U. R. Pothakamury, A. Monsalve-GonzBlez, G. V. Barbosa-Cbovas and B. G. Swansod, Inactivation of Escherichia coli and Staphylococcus aureus in model foods by pulsed electric field technology, Food Research International, vol. 28, no. 2, pp. 167-171, 1995.
• A. Gulbandılar, ‘Investigation of Staphylococcus Aureus Carriage and Antibiotic Susceptibility in Nasal Mucosa in Kütahya Region', Dumlupınar University Journal of Science, no.18, pp. 1-6, 2009.
• P. Boyce, Controlling Tuberculosis Transmission with Ultraviolet Irradiation, Lighting Research Center Publications, 2007.