Comparison of MCM6 rs4988235 Polymorphism Allele and Genotype Frequencies in Professional Football Players and a Sedentary Control Group

Year 2024, Volume: 28 Issue: 5, 1057 - 1063

Beste Tacal Aslan , Tolga Polat , Özlem Özge Yılmaz , Aleyna Muhan , Rukiye Ziya , Korkut Ulucan

Abstract

This study investigated the minichromosome maintenance 6 (MCM6) rs4988235 polymorphism in professional football players, comparing genotype and allele frequencies with a sedentary control group. The control group comprised 64 participants with no history of athletic activity, while the athlete group included 20 football players. DNA extraction from blood samples was performed using a PureLink DNA isolation kit, following the manufacturer's instructions. Real-Time PCR was employed to analyze the MCM6 rs4988235 polymorphism in the isolated DNA. Statistical analysis of the acquired data was conducted using chi-square analysis via IBM SPSS 21.0 software (IBM Statistical Package for Social Sciences Corp., Armonk, NY, USA). A p-value less than 0.05 was considered statistically significant. The CC genotype was absent in all football players. Conversely, CT (10%) and TT (90%) genotypes were identified in 2 and 18 individuals, respectively. In the control group, the distribution of genotypes was as follows: CC (0%), CT (17, 26.6%), and TT (47, 73.4%). The C allele frequency was 5% (2 individuals) in football players and 13.28% (17 individuals) in the control group. The T allele frequency was 95% (38 individuals) in football players and 86.72% (111 individuals) in the control group. No statistically significant differences were observed between the football players and the control group regarding genotype (p = 0.122) or allele frequencies (p = 0.149).

Keywords

MCM6, Lactose, SNP, Football, Genetic

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