KULUÇKA SONRASI DÖNEMDE TAVUK (GALLUS GALLUS DOMESTICA) DUODENUM GOBLET HÜCRELERİNİN HİSTOKİMYASAL YAPISI

Year 2009, Volume: 4 Issue: 1, 40 - 53, 08.06.2009

Dilek Diler Seval Kelek Kenan Çınar

Abstract

Özet: Kuluçkadan sonra 7 ve 60 günlük tavukların (Gallus gallus domestica) duodenumunda yer alan Goblet hücrelerindeki glikoprotein içeriği klasik histokimyasal yöntemlerle belirlendi. Her iki dönemde de villusun tamamındaki lamina epiteliyalis ve kriptlerde yerleşim gösteren Goblet hücrelerinin asidik, nötral, sülfatlı asidik mukosubstans ile O- sülfat esterli ve güçlü sülfatlı glikoproteinleri içerdiği belirlendi. Asidik ve sülfatlı asidik mukosubstansın baskın olduğu gözlendi.

Anahtar kelimeler: Duodenum, Gallus gallus domestica, Goblet hücresi, histokimya

HISTOCHEMICAL CHARACTERIZATION OF MUCOUS CELLS IN THE DUODENUM OF THE CHICKEN (GALLUS GALLUS DOMESTICA) AFTER HATCHING PERIOD

Abstract: The characteristics of glycoproteins in the mucous cells of 7 and 60 days after hatching Gallus gallus domestica duodenum were investigated histochemically using conventional methods. It was determined that mucous cells located in the villus lamina epithelialis and crypts and these cells contained acidic, neutral and sulphated asidic mucosubstance, glycoproteins with O- sulphate esters and very sulphated in both period. Asidic and sulphated asidic mucosubstances were dominant.

Key words: Duodenum, Gallus gallus domestica, mucous cell, histochemistry

Keywords

Duodenum, Gallus gallus domestica, Goblet hücresi, histokimya

References

• AKSOY FT, 1993. Tavuk yetiştiriciliği. Şahin Matbaası, Ankara. pp. 251.
• CULLING CFA, REID PE, DUNN WL, 1976. A new histochemical method for the identification and visualization of both side chain acylated and non-acylated sialic acids. The Journal of Histochemistry and Cytochemistry, 24, 1225-1230.
• GOMARI G, 1952. Gomari’ s Aldehyde Fuchsin Stain. In: CULLING CFA, ALLISON RT. & BARR WT. (Eds.) Cellular Pathology Technique. Butterworths, London. pp. 238.
• KARADAĞ H, NUR İH, 2004. Sindirim Sistemi (Systema Digestorium). In: DURSUN N. (Ed.) Evcil Kuşların Anatomisi. Medisan Yayınevi, Medisan Yayın Serisi: 49, 2. Baskı, Ankara. pp. 230.
• KATSUYAMA T, SPICER SS, 1978. Histochemical differentiation of complex carbohydrates with variants of the concanavalin A-horseradish peroxidase method. The Histochemical Society, 26, 233-250.
• KNOSPE C, 1991. Evidence for secretion channels in the gastric mucous sheet of the cat. Acta Anatomica, 142, 1-5.
• LEV R, SPICER SS, 1964. Specific staining of sulphate groups with alcian blue at low pH. The Journal of Histochemistry and Cytochemistry, 12, 309-309.
• LIÉVIN-LE MOALAD V, SERVIN AL, 2006. The front line of enteric host defense against unwelcome intrusion of harmful microorganisms: Mucins, antimicrobial peptides, and microbiota. Clinical Microbiology Reviews, 19, 315-337.
• LUNA LG, 1968. Methods for Carbohydrates and Mucoproteins. In: LUNA LG. (Ed.) Manual of histologic staining methods of armed forces institute of pathology. McGraw-Hill Book Company, New York. pp. 163-171.
• MC MANUS JFA, 1948. Histological and histochemical uses of periodic acid. Stain Technology, 23, 99-108.
• MOWRY RW, 1956. Alcian blue techniques for the histochemical study of acidic carbohydrates. The Journal of Histochemistry and Cytochemistry, 4, 407-408.
• NOYAN A, 2004. Yaşamda ve hekimlikte fizyoloji. Meteksan anonim şirketi. Ankara. pp. 1154.
• ÖZDEMİR D, AYDIN A, ATALAR Ö, 2004. Dişi kobaylarda (Cavia porcellus) duodenum mukozasının postnatal gelişimi üzerine ışık mikroskopik incelemeler. Fırat Üniversitesi Sağlık Bilimleri Dergisi, 18, 2, 81-84.
• PASTOR LM, BALLESTA J, MADRID JF, PEREZ-TOMAS R, HERNANDEZ F, Acta Histochemica, 83, 91-97.
• ROSE MC, 1992. Mucins: Structure, function and role in pulmonary diseases. Lung Cellular and Molecular Physiology, 263, 413-429.
• SPICER SS, MAYER DR, 1960. Aldehyde Fuchsin/ Alcian Blue. In: CULLING CFA, ALLISON RT. & BARR WT. (Eds.) Cellular Pathology Technique. Butterworths, London. pp. 233.
• UNI Z, SMIRNOV A, SKLAN D, 2003. Pre- and posthatch development of goblet cells in the broiler small ıntestine: effect of delayed access to feed. Poultry Science, 82, 320-327.
• YÖRÜK M, 2008. Sindirim Sistemi II: Sindirim Kanalı. In: ÖZER A. (Ed.) Veteriner Özel Histoloji. Nobel Basımevi, Ankara. pp. 161-184.