Antibacterial Effect of Hypericum atomarium Boiss. Extracts against Clinical Staphylococcus spp.

Year 2018, , 164 - 172, 30.11.2018

Ebru Önem , Halime Çevikbaş

https://doi.org/10.29233/sdufeffd.463587

Cited By: 2

Abstract

Hypericum atomarium Boiss. is a perennial herb in the Hypericum genus. In this
study antibacterial effect of methanol (MeOH), chloroform (CHCl3) and water (H2O) extract of
H. atomarium has been investigated against 19 clinical Staphylococcus spp. (S. aureus, S.
hominis, S. haemolyticus, S. epidermidis). The antibacterial activity of plant extracts was
evaluated using agar well diffusion method. According to the results of this study H. atomarium
extracts displayed strong antibacterial activity against all tested strain and the most antibacterial
effect was determined by chloroform extract with 20,7 mm zone diameter. The evaluation of all
isolates ranged from 20,7-16 mm by chloroform extract. 

Keywords

Antibacterial effect, Staphylococcus spp, Hypericum atomarium Boiss

Hypericum atomarium Boiss. Farklı Çözücü Ekstraktlarının Klinik Stafilokok Türleri Üzerine Antibakteriyel Etkisi

Abstract

Hypericum atomarium Boiss, Hypericum cinsine ait çok yıllık bir bitki olup, geleneksel
tedavi yöntemlerinde tedavi amacıyla kullanılmaktadır. Yapılan bu çalışma ile H. atomarium
Boiss. çiçeklerinden hazırlanan metanol (MeOH), kloroform (CHCl3) ve su (H2O) eksraktlarının
klinik Stafilokok izolatları (S. aureus, S. hominis, S. haemolyticus, S. epidermidis) üzerine
antibakteriyel etkisi araştırılmıştır. Elde edilen sonuçlara göre hazırlanan tüm ekstraktların
klinik izolatlar üzerinde yüksek oranlarda antibakteriyel etkiye sahip oldukları tespit edilmiştir.
En fazla antibakteriyel etki kloroform ile hazırlanan ekstrakta (20,7 mm) tespit edilmiş olup tüm
izolatlar değerlendirildiğinde inhibisyon değerlerinin 20,7-16 mm arasında olduğu tespit
edilmiştir. 

Keywords

Antibakteriyel etki, Stafilokok, Hypericum atomarium Boiss

References

• E. Banin, D. Hughes, and O. P. Kuipers, “Bacterial Pathogens, Antibiotics and Antibiotic Resistance,” FEMS Microbiol. Rev., vol. 41, pp. 450-452, 2017.
• S. Z. Tekin, S. Aksaray, F. Bozkurt, G. Albayrak, and J. Taşçıoğlu, “Çeşitli Klinik Örneklerden İzole Edilen Stafilokok Türlerinde Vankomisin Direncinin Araştırılması,” Fam. Pract. Palliat. Care, vol. 1(2), pp. 43-47, 2016.
• K. Farzana and A. Hameed, “Resistance Pattern of Clinical Isolates of Staphylococcus aureus Against Five Groups of Antibiotics,” Journal of Research (Science), vol. 17(1), pp. 19-26, 2006.
• D. Magilner, M. M. Byerly, and D. M. Cline, “The Prevalence of Community-Acquired Methicillin-Resistant Staphylococcus aureus (CA-MRSA) in Skin Abscesses Presenting to the Pediatric Emergency Department,” N. C. Med. J., vol. 69(5), pp. 351-354, 2008.
• V. Kishor, S. P. Ingole, and B. C. Pathak, “Antibiogram of Staphylococcus aureus from Pus Samples in a Teritary Care Hospital,” Indian Journal of Research, vol. 7(1), pp. 5-7, 2018.
• K. Supré, F. Haesebrouck, R. N. Zadoks, M. Vaneechoutte, S. Piepers, and S. De Vliegher, “Some Coagulase-Negative Staphylococcus Species Affect Udder Health More Than Others,” J. Dairy Sci., vol. 94, pp. 2329–2340, 2011.
• P. R. Fry, J. R. Middleton, S. Dufour, J. Perry, D. Scholl, and I. Dohoo, “Association of Coagulase-Negative Staphylococcal Species, Mammary Quarter Milk Somatic Cell Count, and Persistence of Intramammary Infection in Dairy Cattle,” J. Dairy Sci., vol. 97, pp. 4876–4885, 2014.
• F. Leroy, E. Van Coillie, G. Braem, V. Piessens, B. Vebist, L. De Vuyst, and S. De Vliegher, “Short Communication: Subtyping of Staphylococcus haemolyticus Isolates from Milk and Corresponding Teat Apices to Verify the Potential Teat-Skin Origin of Intramammary Infections in Dairy Cows,” J. Dairy Sci., vol. 98, pp. 7893–7898, 2015.
• E. M. Barros, H. Ceotto, M. C. F. Bastos, K. R. N. dos Santos, and M. Giambiagi-deMarval, “Staphylococcus haemolyticus as an Important Hospital Pathogen and Carrier of Methicillin Resistance Genes,” J. Clin. Microbiol., vol. 50, pp. 166–168, 2012.
• B. A. McManus, D. C. Coleman, E. C. Deasy, G. I. Brennan, B. O’Connell, S. Monecke, R. Ehricht, B. Leggett, N. Leonard, and A. C. Shore, “Comparative Genotypes Staphylococcal Cassette Chromosome mec (SCCmec) Genes and Antimicrobial Resistance amongst Staphylococcus epidermidis and Staphylococcus haemolyticus Isolates from Infections in Humans and Companion Animals,” PLoS One, vol. 10(9), pp. 1-18, 2015.
• C. Vuong and M. Otto, “Staphylococcus epidermidis Infections,” Microbes Infect., vol. 4, pp. 481–489, 2002.
• S. Naik, N. Bouladoux, J. L. Linehan, S. J. Han, O. J. Harrison, C. Wilhelm vd., “Commensal-Dendritic-Cell Interaction Specifies a Unique Protective Skin Immune Signature,” Nature, vol. 520, pp. 104–108, 2015.
• T. H. Nguyen, M. D. Park, and M. Otto, “Host Response to Staphylococcus epidermidis Colonization and Infections,” Front. Cell Infect. Microbiol., vol. 7, p. 90, 2017.
• T. Spanu, M. Sanguinetti, D. Ciccaglione, T. D’Inzeo, L. Romano, F. Leone, and G. Fadda, “Use of the VITEK 2 System for Rapid Identification of Clinical Isolates of Staphylococci from Bloodstream Infections,” J. Clin. Microbiol., vol. 41, pp. 4259–4263, 2003.
• F. Chaves, A. Garcı´a, M. Alvarez, F. Sanz, C. Alba, and J. R. Otero, “Nosocomial Spread of a Staphylococcus hominis subsp. novo-biosepticus Strain Causing Sepsis in a Neonatal Intensive Care Unit,” J Clin. Microbiol., vol. 43, pp. 4877-4879, 2005.
• P. A. d’Azevedo, R. Trancesi, T. Sales, J. Monteiro, A.C. Gales, and A. C. Pignatari, “Outbreak of Staphylococcus hominis subsp. novobiosepticus Bloodstream Infections in Sao Paulo City, Brazil,” J. Med. Microbiol., vol. 57, pp. 256–257, 2008.
• A. Sorlozano, J. Gutierrez, T. Martinez, M. E. Yuste, J. A. Perez-Lopez, A. Vindel, J. Guillen, and T. Boquete, “Detection of New Mutations Conferring Resistance to Linezolid in Glycopeptide- Intermediate Susceptibility Staphylococcus hominis subspecies hominis Circulating in an Intensive Care Unit,” Eur. J. Clin. Microbiol. Infect. Dis., vol. 29, pp. 73–80, 2010.
• E. Ruiz de Gopegui, C. Iuliana Marinescu, P. Diaz, A. Socias, and M. Garau, “Nosocomial Spread of Linezolid-Resistant Staphylococcus hominis in Two Hospitals in Majorca,” Enferm. Infecc. Microbiol. Clin., vol. 29, pp. 339–344, 2011.
• Y. Kourkoutas, N. Chorianopoulos, and P. D. Ciccio, “Bioactive Natural Products 2018,” Biomed. Res. Int., vol. 111, pp. 1259–1265, 2018.
• A. Milshteyn, D. A. Colosimo, and S. F. Brady, “Accessing Bioactive Natural Products from the Human Microbiome,” Cell Host Microbe, vol. 23, pp. 725-736, 2018.
• A. Redvers, R. Laugharne, G. Kanagaratnam, and G. Srinivasan, “How Many Patients Self-Medicate with St John's Wort?” Psychiatr. Bull., vol. 25, pp. 254-56, 2001.
• B. Gudzic. S. Dordevic, V. Nedellkovic, and A. Smelcerovic, “Essential Oils Composition of Hypericum atomarium Boiss.” Chemistry Indian, vol. 58(9), pp. 413-415, 2004.
• S. Ulusoy, G. Boşgelmez-Tınaz, and H. Secilmiş-Canbay, “Tocopherol, Carotene, Phenolic Contents and Antibacterial Properties Rose Essential Oil, Hydrosol and Absolute,” Curr. Microbiol., vol. 59, pp. 554-558, 2009.
• C. Avşar, H. Keskin, and İ. Berber, “Hastane İnfeksiyonlarından İzole Edilen Mikroorganizmalara Karşı Bazı Bitki Ekstraktlarının Antimikrobiyal Aktivitesi,” Int. J. Pure Appl. Sci., vol. 2(1), pp. 22-29, 2016.
• O. Stefanovic, L. Comic, D. Stanojevic, and S. Solujic, “Antibacterial Activity of Aegopodium podagraria L. Extracts and Interaction Between Extracts and Antibiotics,” Turk. J. Biol., vol. 33(2), pp. 145-50, 2009.
• İ. Berber, C. Avşar, N. Çine, N. Bozkurt, and E. Elmas, “Sinop’da Yetişen Bazı Bitkilerin Metanolik Ekstraktlarının Antibakteriyal ve Antifungal Aktivitelerinin Belirlenmesi,” Karaelmas Fen Müh. Derg., vol. 3(1), pp. 10-6, 2013.
• N. A. A. Mohd Nazri, N. Ahmat, A. Adnan, S. A. Syed, and S. A. Syaripah, “In Vitro Antibacterial and Radical Scavenging Activities of Malaysian Table Salad,” Afr. J. Biotech., vol. 10(30), pp. 5728-35, 2011.
• Z. Saddiqe, I. Naeem, I, and A. Maimoona, “A Review of the Antibacterial Activity of Hypericum perforatum L.,” J. Ethnopharmacol., vol. 131( 3), pp. 511-521, 2010.
• A. E. Erdoğan and A. Everest, “Antimikrobiyal Ajan Olarak Bitki Bileşenleri,” Türk Bilimsel Derlemeler Dergisi, vol. 6 (2), pp. 27-32, 2013.
• O. Keleş, S. Ak, T. Bakırel, and K. Alpınar, “Türkiyede Yetişen Bazı Bitkilerin Antibakteriyel Etkisinin İncelenmesi,” Turk. J. Vet. Anim. Sci., vol. 25, pp. 559-565, 2001.
• O. Tusevski, M. Krstikj, J. P. Stanoeva, M. Stefova, and G. S. Simic, “Phenolic Profile and Biological Activity of Hypericum perforatum L.: Can Roots Be Considered as a New Source of Natural Compounds?” S. Afr. J. Bot., vol. 117, pp. 301–310, 2018.
• T. Beveridge, “Structures of Gram-Negative Cell Walls and Their Derived Membrane Vesicles,” J. Bacteriol., vol. 181(16), p. 4725, 1999.
• R. Khan, B. Islam, M. Akram, S. Shakil, A. Ahmad, S. M. Ali, M. Siddiqui, and A. U. Khan, “Antimicrobial Activity of Five Herbal Extracts Against Multi Drug Resistant (MDR) Strains of Bacteria and Fungus of Clinical Origin,” Molecules, vol. 14, pp. 586-597, 2009.
• M. Masoumian and M. Zandi, “Antimicrobial Activity of Some Medicinal Plant Extracts against Multidrug Resistant Bacteria,” J. Res. Med. Sci., vol. 19(11), p. 10080, 2017.