Süleyman Demirel University Faculty of Arts and Science Journal of Science
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İdrarda Orotik Asit’in Temassız İletkenlik Dedektörü Kullanılarak Kapiler Elektroforez Yöntemi İle Hızlı Tayini

Year 2020, Volume 15, Issue 2, 194 - 202, 29.11.2020

Mehmet Şerif CANSEVER Nevin ÖZTEKİN Veselina ADIMCILAR Tanyel ZÜBARİOĞLU Ertuğrul KIYKIM Ayşe AKTUĞLU

https://doi.org/10.29233/sdufeffd.695869

Abstract

İdrarda orotik asidin aşırı atılımı üre döngüsü bozukluğu ve pirimidin sentez bozukluğu gibi doğumsal metabolik hastalıklarda görülür. Bu nedenle orotik asidin hızlı bir şekilde tayini hayati öneme sahiptir. Bu çalışmada idrar örneklerinde orotik asidin kantitatif düzeylerinin belirlenmesi için yeni, basit, ucuz ve hızlı bir kapiler elektroforetik analiz yöntemi geliştirildi. Orotik asidin temassız iletkenlik dedektörü (C4D) kullanılarak kapiler elektroforez yöntemi ile ayrımı için pH’ı 6,5 olan 20 mM 4-Morfolinetansülfonik asit (MES), 10 mM Histidin ve 0,1 mM setil trimetilamonyum bromür (CTAB) içeren bir ça-lışma tamponu ile geliştirildi. Orotik asidin ayrılması yaklaşık 3,5 dakika gibi kısa bir sürede tamamlandı. Göç zamanlarının ve düzeltilmiş pik alanlarının tekrarlanabilirliği sırasıyla %RSD olarak 1,25 ve 4,31 olarak bulundu. En düşük dedeksiyon limiti (LOD) 0,733 mg / L ve en düşük tayin limiti de (LOQ) 2,443 mg / L olarak bulundu. Metodun idrarda orotik asit düzeylerinin tayin edilmesinde kolaylıkla ve hassasiyetle kullanılabileceği gösterildi.

Keywords

Kapiler Elektroforez, Orotik Asit, Temassız İletkenlik Dedektörü (C4D), Üre Döngüsü Bozuklukları, Doğumsal Metabolik Hastalık, İdrar

References

  • [1] M. Duran, L. Dorland, E. E. Meuleman, P. Allers, R. Berger, ‘’Inherited defects of purine and pyrimidine metabolism: laboratory methods for diagnosis,’’ J Inherit Metab Dis 20:227–236, 1997.
  • [2] M. Loeffler, E. Zameitat, ‘’Pyrimidine biosynthesis’’, Encycl Biol Chem, 3:600–605, 2004
  • [3] S. Balasubramaniam, J.A. Duley, J. Christodoulou, ‘’Inborn errors of pyrimidine metabolism: clinical update and therapy,’’ J. Inherit. Metab. Dis, 37(5):687-98, 2014.
  • [4] R.E. Kelley, H.C. Andersson, ‘’Disorders of purines and pyrimidines,’’. Handb Clin Neurol., 120:827- 38, 2014.
  • [5] J. Häberle, N. Boddaert, A. Burlina, A. Chakrapani, M. Dixon, M. Huemer, D. Karall, D. Martinelli , P.S. Crespo, R: Santer, A. Servais, V. Valayannopoulos, M. Lindner , V. Rubio disorders,’’ Orphanet J Rare Dis, 29;7-32, 2012.
  • [6] C. Bachmann, J.P. Colombo. ‘’Diagnostic value of orotic acid excretion in heritable disorders of the urea cycle and in hyperammonemia due to organic acidurias,’’ Eur J Pediatr., 134(2):109-13,1980.
  • [7] S.W. Brusilow, E. Hauser. ‘’Simple method of measurement of orotic acid and orotidine in urine,’’ J. Chromatogr., 1;493(2):388-91, 1989.
  • [8] M. Löffler , E.A. Carrey , E. Zameitat. ‘’Orotic Acid, More Than Just an Intermediate of Pyrimidine de novo Synthesis’’. J Genet Genomics., 20;42(5):207-19, 2015.
  • [9] C. Salerno, C. Crifò, ‘’Diagnostic value of urinary orotic acid levels: applicable separation methods,’ J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci., 781, 57–71, 2002
  • [10] S. Yin, S. Dragusha, V. Ejupi, T. Shibata, T. Kabashima, M. Kai, ‘’Sensitive and selectivedetermina-tion of orotic acid in biological specimens using a novel fluorogenicreaction,’’ J. Fluoresc., 25; 1005–1011, 2015
  • [11] A. Fioravanti, M. Flaviani, C. Gambelunghe, A. Micheletti, M. Sposito, S. Rufini, ‘’High-performance Liquid Chromatographic Determination of Orotic Acid as its Methyl Derivative in Human Urine’’ Journal of chromatography. B, Biomedical sciences and applications, vol. 703, 263–266, 1997
  • [12] B. Tavazzi, G. Lazzarino, P. Leone, A.M. Amorini, F. Bellia, C.G. Janson, et al.,’’Simultaneous high performance liquid chromatographic separation of purines, pyrimidines, N-acetylated amino acids,and dicarboxylic acids for the chemical diagnosis of inborn errors of metabolism,’’ Clin. Biochem. 38, 997–1008, 2005
  • [13] M.S. Rashed, M. Jacob, M. Al-Amoudi, Z. Rahbeeni, M.A.D. Al-Sayed, L. Al-Ahaidib, et al., ‘’Rapid determination of orotic acid in urine by liquid chromatography-electrospray tandem mass spectro-metry,’’ Clin. Chem., 49, 499–501, 2003.
  • [14] G. la Marca, B. Casetta, E. ‘’Zammarchi, Rapid determination of orotic acid in urineby a fast liquid chromatography/tandem mass spectrometric method’’, Rapid Commun. Mass Spectrom., 17, 788-793, 2003.
  • [15] O. D'Apolito, D. Garofalo, G. Paglia, A. Zuppaldi, G. Corso, ‘’Orotic acid quantification in dried blood spots and biological fluids by hydrophilic interaction liquid chromatography tandem mass spectrometry’’, J. Separ. Sci., 33, 966–973,2010.
  • [16] R. Hušková, P. Barták, L. Čáp, D. Friedecký, T. Adam, ‘’Analytical derivatization-a tool for deter-mination of orotic acid’’, J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 799, 303–309,2004.
  • [17] O. Giardini, C. Salerno, P. D. Eufemia, M. Celli, R. Finocchiaro, C. Crifo, ‘’Determination of urinary orotic acid and uracil by capillary zone electrophoresis’’, J. Chromatogr. B., 734,175–178,1999
  • [18] J. Sevcík, T. Adam, V. Sázel, ‘’A rapid and simple screening method for detection of orotic aciduria by capillary zone electrophoresis,’’ Clin. Chim. Acta ,259,73–81, 1997.
  • [19] H. Khajehsharifi, H. Tavallali, M. Shekoohi, M. Sadeghi, ‘’Spectrophotometric simultaneous deter-mination of orotic acid, creatinine and uric acid by orthogonal signal correction-partial least squares in spiked real samples’’, Drug Test. Anal., 5, 353–360, 2013.
  • [20] J. W. Jorgenson, K. D. Lukacs,. “Zone electrophoresis in open-tubular glass capillaries,” Analytical Chemistry, 53, 1298– 1302,1981
  • [21] J. W. Jorgenson, K. D. Lukacs. “Free-zone electrophoresis in glass capillaries,” Clinical Chemistry, 27, 1551-1553,1981.
  • [22] J. W. Jorgenson, K. D. Lukacs,. “High-resolution separations based on electrophoresis and electroosmosis,” Journal of Chromatography A, 218, 209-216, 1981.
  • [23] S. F. Y., Capillary Electrophoresis. Amsterdam: Elsevier, 1992.
  • [24] S. Özden, R. Ertan, E. Akı-Şener, G. Yalçın, Farmasötik Kimya Pratikleri. Ankara. Ankara Üniversi-tesi, Eczacılık Fakültesi, 1-2, 2004.
  • [25] P. Kubáň, P. C. Hauser, “Contactless conductivity detection in capillary electrophoresis: A review,” Electroanalysis, 16(24), 2009-2021, 2004.
  • [26] A. J. Zemann, E. Schnell, D. Volgger, G. K. Bonn, ‘’Conductivity detection for capillary electrophoresis,” Analytical Chemistry, 70(3), 563-567, 1998.
  • [27] H.E. Yelkenci, N. Öztekin, ‘’Separation and Sensitive Detection of Lanthanides by Capillary Elect-rophoresis and Contactless Conductivity Detection,’’ Journal of Chromatographic Science, 55 (4), 465– 470, 2017.
  • [28] P. Kubáň, P. C. Hauser, “Review: Capacitively coupled contactless conductivity detection for microseparation techniques-recent developments, ” Electrophoresis, 32, 30-42, 2011.
  • [29] P.Kubáň, P. C. Hauser, “Ten years of axial capacitively coupled contactless conductivity detection for CZE - a review,” Electrophoresis, 30, 176-188, 2009.
  • [30] P. Kubáň, P. C. Hauser, “A review of the recent achievements in capacitively coupled contactless conductivity detection,” Analytica Chimica Acta, 607(1), 15-29, 2008.
  • [31] A. A. Elbashir, H. Y. Aboul-Enein, “Recent advances in applications of capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D): An update,” Biomedical Chro-matography, 26, 990-1000, 2012.

Year 2020, Volume 15, Issue 2, 194 - 202, 29.11.2020

Mehmet Şerif CANSEVER Nevin ÖZTEKİN Veselina ADIMCILAR Tanyel ZÜBARİOĞLU Ertuğrul KIYKIM Ayşe AKTUĞLU

https://doi.org/10.29233/sdufeffd.695869

Abstract

References

  • [1] M. Duran, L. Dorland, E. E. Meuleman, P. Allers, R. Berger, ‘’Inherited defects of purine and pyrimidine metabolism: laboratory methods for diagnosis,’’ J Inherit Metab Dis 20:227–236, 1997.
  • [2] M. Loeffler, E. Zameitat, ‘’Pyrimidine biosynthesis’’, Encycl Biol Chem, 3:600–605, 2004
  • [3] S. Balasubramaniam, J.A. Duley, J. Christodoulou, ‘’Inborn errors of pyrimidine metabolism: clinical update and therapy,’’ J. Inherit. Metab. Dis, 37(5):687-98, 2014.
  • [4] R.E. Kelley, H.C. Andersson, ‘’Disorders of purines and pyrimidines,’’. Handb Clin Neurol., 120:827- 38, 2014.
  • [5] J. Häberle, N. Boddaert, A. Burlina, A. Chakrapani, M. Dixon, M. Huemer, D. Karall, D. Martinelli , P.S. Crespo, R: Santer, A. Servais, V. Valayannopoulos, M. Lindner , V. Rubio disorders,’’ Orphanet J Rare Dis, 29;7-32, 2012.
  • [6] C. Bachmann, J.P. Colombo. ‘’Diagnostic value of orotic acid excretion in heritable disorders of the urea cycle and in hyperammonemia due to organic acidurias,’’ Eur J Pediatr., 134(2):109-13,1980.
  • [7] S.W. Brusilow, E. Hauser. ‘’Simple method of measurement of orotic acid and orotidine in urine,’’ J. Chromatogr., 1;493(2):388-91, 1989.
  • [8] M. Löffler , E.A. Carrey , E. Zameitat. ‘’Orotic Acid, More Than Just an Intermediate of Pyrimidine de novo Synthesis’’. J Genet Genomics., 20;42(5):207-19, 2015.
  • [9] C. Salerno, C. Crifò, ‘’Diagnostic value of urinary orotic acid levels: applicable separation methods,’ J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci., 781, 57–71, 2002
  • [10] S. Yin, S. Dragusha, V. Ejupi, T. Shibata, T. Kabashima, M. Kai, ‘’Sensitive and selectivedetermina-tion of orotic acid in biological specimens using a novel fluorogenicreaction,’’ J. Fluoresc., 25; 1005–1011, 2015
  • [11] A. Fioravanti, M. Flaviani, C. Gambelunghe, A. Micheletti, M. Sposito, S. Rufini, ‘’High-performance Liquid Chromatographic Determination of Orotic Acid as its Methyl Derivative in Human Urine’’ Journal of chromatography. B, Biomedical sciences and applications, vol. 703, 263–266, 1997
  • [12] B. Tavazzi, G. Lazzarino, P. Leone, A.M. Amorini, F. Bellia, C.G. Janson, et al.,’’Simultaneous high performance liquid chromatographic separation of purines, pyrimidines, N-acetylated amino acids,and dicarboxylic acids for the chemical diagnosis of inborn errors of metabolism,’’ Clin. Biochem. 38, 997–1008, 2005
  • [13] M.S. Rashed, M. Jacob, M. Al-Amoudi, Z. Rahbeeni, M.A.D. Al-Sayed, L. Al-Ahaidib, et al., ‘’Rapid determination of orotic acid in urine by liquid chromatography-electrospray tandem mass spectro-metry,’’ Clin. Chem., 49, 499–501, 2003.
  • [14] G. la Marca, B. Casetta, E. ‘’Zammarchi, Rapid determination of orotic acid in urineby a fast liquid chromatography/tandem mass spectrometric method’’, Rapid Commun. Mass Spectrom., 17, 788-793, 2003.
  • [15] O. D'Apolito, D. Garofalo, G. Paglia, A. Zuppaldi, G. Corso, ‘’Orotic acid quantification in dried blood spots and biological fluids by hydrophilic interaction liquid chromatography tandem mass spectrometry’’, J. Separ. Sci., 33, 966–973,2010.
  • [16] R. Hušková, P. Barták, L. Čáp, D. Friedecký, T. Adam, ‘’Analytical derivatization-a tool for deter-mination of orotic acid’’, J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 799, 303–309,2004.
  • [17] O. Giardini, C. Salerno, P. D. Eufemia, M. Celli, R. Finocchiaro, C. Crifo, ‘’Determination of urinary orotic acid and uracil by capillary zone electrophoresis’’, J. Chromatogr. B., 734,175–178,1999
  • [18] J. Sevcík, T. Adam, V. Sázel, ‘’A rapid and simple screening method for detection of orotic aciduria by capillary zone electrophoresis,’’ Clin. Chim. Acta ,259,73–81, 1997.
  • [19] H. Khajehsharifi, H. Tavallali, M. Shekoohi, M. Sadeghi, ‘’Spectrophotometric simultaneous deter-mination of orotic acid, creatinine and uric acid by orthogonal signal correction-partial least squares in spiked real samples’’, Drug Test. Anal., 5, 353–360, 2013.
  • [20] J. W. Jorgenson, K. D. Lukacs,. “Zone electrophoresis in open-tubular glass capillaries,” Analytical Chemistry, 53, 1298– 1302,1981
  • [21] J. W. Jorgenson, K. D. Lukacs. “Free-zone electrophoresis in glass capillaries,” Clinical Chemistry, 27, 1551-1553,1981.
  • [22] J. W. Jorgenson, K. D. Lukacs,. “High-resolution separations based on electrophoresis and electroosmosis,” Journal of Chromatography A, 218, 209-216, 1981.
  • [23] S. F. Y., Capillary Electrophoresis. Amsterdam: Elsevier, 1992.
  • [24] S. Özden, R. Ertan, E. Akı-Şener, G. Yalçın, Farmasötik Kimya Pratikleri. Ankara. Ankara Üniversi-tesi, Eczacılık Fakültesi, 1-2, 2004.
  • [25] P. Kubáň, P. C. Hauser, “Contactless conductivity detection in capillary electrophoresis: A review,” Electroanalysis, 16(24), 2009-2021, 2004.
  • [26] A. J. Zemann, E. Schnell, D. Volgger, G. K. Bonn, ‘’Conductivity detection for capillary electrophoresis,” Analytical Chemistry, 70(3), 563-567, 1998.
  • [27] H.E. Yelkenci, N. Öztekin, ‘’Separation and Sensitive Detection of Lanthanides by Capillary Elect-rophoresis and Contactless Conductivity Detection,’’ Journal of Chromatographic Science, 55 (4), 465– 470, 2017.
  • [28] P. Kubáň, P. C. Hauser, “Review: Capacitively coupled contactless conductivity detection for microseparation techniques-recent developments, ” Electrophoresis, 32, 30-42, 2011.
  • [29] P.Kubáň, P. C. Hauser, “Ten years of axial capacitively coupled contactless conductivity detection for CZE - a review,” Electrophoresis, 30, 176-188, 2009.
  • [30] P. Kubáň, P. C. Hauser, “A review of the recent achievements in capacitively coupled contactless conductivity detection,” Analytica Chimica Acta, 607(1), 15-29, 2008.
  • [31] A. A. Elbashir, H. Y. Aboul-Enein, “Recent advances in applications of capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D): An update,” Biomedical Chro-matography, 26, 990-1000, 2012.

Details

Primary Language Turkish
Subjects Engineering, Chemical
Journal Section Makaleler
Authors

Mehmet Şerif CANSEVER (Primary Author)
İSTANBUL ÜNİVERSİTESİ- CERRAHPAŞA
0000-0003-3315-5124
Türkiye

Nevin ÖZTEKİN
İSTANBUL TEKNİK ÜNİVERSİTESİ
0000-0003-3676-5147
Türkiye

Veselina ADIMCILAR
İSTANBUL TEKNİK ÜNİVERSİTESİ
0000-0002-7016-1200
Türkiye

Tanyel ZÜBARİOĞLU
SAĞLIK BİLİMLERİ ÜNİVERSİTESİ
0000-0002-7159-4008
Türkiye

Ertuğrul KIYKIM
İSTANBUL ÜNİVERSİTESİ - CERRAHPAŞA
0000-0001-5170-5594
Türkiye

Ayşe AKTUĞLU
İSTANBUL ÜNİVERSİTESİ - CERRAHPAŞA
0000-0001-7256-0750
Türkiye

Publication Date November 29, 2020
Published in Issue Year 2020, Volume 15, Issue 2

Cite

Bibtex @research article { sdufeffd695869, journal = {Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi}, issn = {}, eissn = {1306-7575}, address = {}, publisher = {Süleyman Demirel University}, year = {2020}, volume = {15}, pages = {194 - 202}, doi = {10.29233/sdufeffd.695869}, title = {İdrarda Orotik Asit’in Temassız İletkenlik Dedektörü Kullanılarak Kapiler Elektroforez Yöntemi İle Hızlı Tayini}, key = {cite}, author = {Cansever, Mehmet Şerif and Öztekin, Nevin and Adımcılar, Veselina and Zübarioğlu, Tanyel and Kıykım, Ertuğrul and Aktuğlu, Ayşe} }
APA Cansever, M. Ş. , Öztekin, N. , Adımcılar, V. , Zübarioğlu, T. , Kıykım, E. & Aktuğlu, A. (2020). İdrarda Orotik Asit’in Temassız İletkenlik Dedektörü Kullanılarak Kapiler Elektroforez Yöntemi İle Hızlı Tayini . Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi , 15 (2) , 194-202 . DOI: 10.29233/sdufeffd.695869
MLA Cansever, M. Ş. , Öztekin, N. , Adımcılar, V. , Zübarioğlu, T. , Kıykım, E. , Aktuğlu, A. "İdrarda Orotik Asit’in Temassız İletkenlik Dedektörü Kullanılarak Kapiler Elektroforez Yöntemi İle Hızlı Tayini" . Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi 15 (2020 ): 194-202 <https://dergipark.org.tr/en/pub/sdufeffd/issue/57875/695869>
Chicago Cansever, M. Ş. , Öztekin, N. , Adımcılar, V. , Zübarioğlu, T. , Kıykım, E. , Aktuğlu, A. "İdrarda Orotik Asit’in Temassız İletkenlik Dedektörü Kullanılarak Kapiler Elektroforez Yöntemi İle Hızlı Tayini". Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi 15 (2020 ): 194-202
RIS TY - JOUR T1 - İdrarda Orotik Asit’in Temassız İletkenlik Dedektörü Kullanılarak Kapiler Elektroforez Yöntemi İle Hızlı Tayini AU - Mehmet Şerif Cansever , Nevin Öztekin , Veselina Adımcılar , Tanyel Zübarioğlu , Ertuğrul Kıykım , Ayşe Aktuğlu Y1 - 2020 PY - 2020 N1 - doi: 10.29233/sdufeffd.695869 DO - 10.29233/sdufeffd.695869 T2 - Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi JF - Journal JO - JOR SP - 194 EP - 202 VL - 15 IS - 2 SN - -1306-7575 M3 - doi: 10.29233/sdufeffd.695869 UR - https://doi.org/10.29233/sdufeffd.695869 Y2 - 2020 ER -
EndNote %0 Süleyman Demirel University Faculty of Arts and Science Journal of Science İdrarda Orotik Asit’in Temassız İletkenlik Dedektörü Kullanılarak Kapiler Elektroforez Yöntemi İle Hızlı Tayini %A Mehmet Şerif Cansever , Nevin Öztekin , Veselina Adımcılar , Tanyel Zübarioğlu , Ertuğrul Kıykım , Ayşe Aktuğlu %T İdrarda Orotik Asit’in Temassız İletkenlik Dedektörü Kullanılarak Kapiler Elektroforez Yöntemi İle Hızlı Tayini %D 2020 %J Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi %P -1306-7575 %V 15 %N 2 %R doi: 10.29233/sdufeffd.695869 %U 10.29233/sdufeffd.695869
ISNAD Cansever, Mehmet Şerif , Öztekin, Nevin , Adımcılar, Veselina , Zübarioğlu, Tanyel , Kıykım, Ertuğrul , Aktuğlu, Ayşe . "İdrarda Orotik Asit’in Temassız İletkenlik Dedektörü Kullanılarak Kapiler Elektroforez Yöntemi İle Hızlı Tayini". Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi 15 / 2 (November 2020): 194-202 . https://doi.org/10.29233/sdufeffd.695869
AMA Cansever M. Ş. , Öztekin N. , Adımcılar V. , Zübarioğlu T. , Kıykım E. , Aktuğlu A. İdrarda Orotik Asit’in Temassız İletkenlik Dedektörü Kullanılarak Kapiler Elektroforez Yöntemi İle Hızlı Tayini. Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi. 2020; 15(2): 194-202.
Vancouver Cansever M. Ş. , Öztekin N. , Adımcılar V. , Zübarioğlu T. , Kıykım E. , Aktuğlu A. İdrarda Orotik Asit’in Temassız İletkenlik Dedektörü Kullanılarak Kapiler Elektroforez Yöntemi İle Hızlı Tayini. Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi. 2020; 15(2): 194-202.
IEEE M. Ş. Cansever , N. Öztekin , V. Adımcılar , T. Zübarioğlu , E. Kıykım and A. Aktuğlu , "İdrarda Orotik Asit’in Temassız İletkenlik Dedektörü Kullanılarak Kapiler Elektroforez Yöntemi İle Hızlı Tayini", Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi, vol. 15, no. 2, pp. 194-202, Nov. 2020, doi:10.29233/sdufeffd.695869
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