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Oskar Balığının (Astronotus ocellatus) Solungaçlarında Glikokonjugatların Dağılımı

Year 2021, Volume: 16 Issue: 2, 424 - 433, 25.11.2021
https://doi.org/10.29233/sdufeffd.895449

Abstract

Pek çok fonksiyona katılan glikokonjugatlar, birçok hücre tipi tarafından üretilir. Bu çalışmada Oscar balığı (Astronotus ocellatus) solungaçlarındaki glikokonjugat varlığının beş horseradish peroksidaz ile konjuge lektin kullanarak araştırılması amaçlandı. Bu çalışma, solungaç epitel hücrelerinin Canavalia ensiformis agglutinin (Con A) ve Arachis hypogaea agglutinin (PNA) ile reaksiyona girdiğini ortaya çıkardı. Solungaç primer lamellerinin epitel hücrelerinde Con A’ya karşı güçlü reaksiyon görüldü. Primer ve sekonder lamel epitel hücreleri ve eozinofilik granüler hücreler, değişen yoğunlukta PNA bağlanmasına sahipti. Primer lamellerin bağ dokusunda Ulex europaeus agglutinin-I (UEA-I) pozitif eozinofilik granüler hücreler gözlendi. Solungaç primer filamentinin epitel hücreleri, çeşitli yoğunluklarda Con A reaksiyonuna sahipti. Yüzeysel epitel hücreleri PNA’ya karşı çeşitli derecelerde reaksiyon göstermekteydi. Sekonder lamellerin epitel hücreleri Con A ve PNA pozitif reaksiyona sahipti. Bu sonuçlar, glikokonjugat dağılımının türler arası varyasyonlar gösterebileceğine işaret etmektedir, bu da Oscar balık solungaçlarının epitelyal mukoza hücrelerinde doğal olarak oluşan glikokonjugatların yüksek heterojenitesinin, glikokonjugatların çeşitli fonksiyonel rollerine dayandırılabileceğini düşündürmektedir.

References

  • [1] N. Heisler, “Acid-base regulation in response to changes of the environment characteristics and capacity,” in Fish Ecophysiology, J. C. Rankin, F. B. Jensen, Eds. London: Chapman-Hall, 1993, pp. 207-230.
  • [2] W. S. Hoar and D. J. Randall, “Gills-Anatomy, gas transfer, and acid-base regulation,” in Fish Physiology, vol. XA, New York: Academic Press, 1984, p. 456.
  • [3] W. S. Hoar and D. J. Randall, “Gills-Ion and water transfer,” in Fish Physiology, vol. XB, New York: Academic Press, 1984, p. 416p.
  • [4] E. M. Zuchelkowski, R. C. Lantz, and D. E. Hinton, “Effects of acid-stress on epidermal mucous cells of the brown bullhead Ictalurus nebulosus: A morphometric study,” The Anatomical Record, 200, 33-39, 1981.
  • [5] E. M. Zuchelkowski, C. A. Pinkstaff, and D. E. Hinton, “Mucosubstance histochemistry in control and acid-stressed epidermis of brown bullhead cat fish, Ictalurus nebulosus,” The Anatomical Record, 212, 327-335, 1985.
  • [6] K. L. Shephard, “Functions for fish mucus,” Reviews in Fish Biology and Fisheries, 4, 401-29, 1994.
  • [7] A. Allen, A. Bell, M. Mantle, and J. P. Pearson, “The structure and physiology of gastrointestinal mucus,” Advances in Experimental Medicine and Biology, 144, 115-133, 1982.
  • [8] M. E. V. Johansson, H. Sjövall, and G. C. Hansson, “The gastrointestinal mucus system in health and disease,” Nature Reviews-Gastroenterology and Hepatology, 10, 352-361, 2013.
  • [9] G. J. Strous and J. Dekker, “Mucin-type glycoproteins,” Critical Reviews in Biochemistry and Molecular Biology, 27, 57-92, 1992.
  • [10] F. Genten and A. Danguy, “Light microscopic characterization of glycoconjugates and cell proliferation in teleost epidermis exposed to acute levels of pH,” Zeitschrift fur Mikroskopisch-Anatomische Forschung, 104, 119-139, 1990.
  • [11] S.A. Brooks, “Lectin Histochemistry: Historical Perspectives, State of the Art, and the Future,” Methods in Molecular Biology, 1560, 93-107, 2017.
  • [12] S. M. T. Saboaia‐Moraes, F. J. Hernandez‐Blazquez, D. L. Mota, and A.M. Bittencourt, “Mucous cell types in the branchial epithelium of the euryhaline fish Poecilia vivipara,” Journal of Fish Biology, 49 (3), 545-548. 1996.
  • [13] K. Çınar, N. Şenol, and M. R. Özen, “Histochemical characterization of glycoproteins in the gill of the carp (Cyprinus carpio),” Ankara Üniversitesi Veteriner Fakültesi Dergisi, 55, 61-64, 2008.
  • [14] A. O. Diaz, A. M. Garcia, C. V. Devincenti, and A. L. Goldemberg, “Ultrastructure and histochemical study of glycoconjugates in the gills of the white croaker (Micropogonias furnieri),” Anatomia, Histologia, Embryologia, 34, 117-122, 2005.
  • [15] A. O. Diaz, A. M. Garcia, A. H. Escalante, and A. L. Goldemberg, “Glycoconjugates in the branchial mucous cells of Cynoscion guatucupa,” Scientia Marina, 69 (4), 545-553, 2005.
  • [16] D. M. Fracalossi, M. E. Allen, D. K. Nichols, and O. T. Oftedal, “Oscars, Astronotus ocellatus, have a dietary requirement for vitamin C,” The Journal of Nutrition, 128 (10), 1745-1751, 1998.
  • [17] M. E. de Jesus Trindade, and H. L. de Queiroz, “Feeding ecology and morphometry of the digestive tract of Astronotus ocellatus (Cichlidae) in Várzea environments of the Middle Solimões Region, Central Amazon, Brazil,” Scientific Magazine UAKARI, 8, 45-57, 2012.
  • [18] K. Çınar, M. Öztop, and B. Özkarasu, “Glycoconjugate composition of ovine parotid glands elucidated by lectins,” Journal of Morphological Sciences, 33 (1), 8-13, 2016.
  • [19] M. Öztop, K. Çınar, and E. Demirbağ, “Lectin histochemistry of the glycoconjugates in partridge and quail conjunctival epithelia,” Van Veterinary Journal, 32(1), 1-6, 2021.
  • [20] I. E. Liener, N. Sharon, and I. J. Goldstein, The Lectins: Properties, Functions, and Applications in Biology and Medicine. Orlando, Florida: Academic Press, 1986, p. 618.
  • [21] C. Purbomartono, A. Takemura, and K. Takano, “Histochemical study of mucus lectin distribution on several tissues of tilapia (Oreochromis mossambicus),” Jurnal Perikanan (Journal of Fisheries Sciences), 9 (1), 1-7, 2007.
  • [22] A. O. Díaz, A. M. García, A. H. Escalante, and A. L. Goldemberg, “Glycoproteins histochemistry of the gills of Odontesthes bonariensis (Teleostei, Atherinopsidae),” Journal of Fish Biolology, 77 (7), 1665-1673, 2010.
  • [23] M. C. Rojo, M. J. Blanquez, and M. E. Gonzalez, “A histochemical study of the distribution of lectin binding sites in the developing branchial area of the trout Salmo trutta,” Journal of Anatomy, 189 (3), 609-621, 1996.
  • [24] P. Burkhardt-Holm, “Lectin histochemistry of rainbow trout (Oncorhynchus mykiss) gill and skin,” The Histochemical Journal, 29, 893-899, 1997.
  • [25] G.G. Goss, S. Adamia, and F. Galvez, “Peanut lectin binds to a subpopulation of mitochondria-rich cells in the rainbow trout gill epithelium”, American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 281, 1718-1725, 2001.
  • [26] C. Calabrò, M. P. Albanese, E. R. Lauriano, S. Martella, and A. Licata, “Morphological, histochemical and immunohistochemical study of the gill epithelium in the abyssal teleost fish Coelorhynchus coelorhynchus,” Folia Histochemica et Cytobiologica, 43(1), 51-56, 2005.
  • [27] K. S. Jung, M. J. Ahn, Y. D. Lee, G. M. Go, and T. K. Shin, “Histochemistry of six lectins in the tissues of the flat fish Paralichthys olivaceus,” Journal of Veterinary Science, 3 (4), 292-301, 2002.
  • [28] G. Kato, H. Miyazawa, Y. Nakayama, Y. Ikari, H. Kondo, T. Yamaguchi, M. Sano, and U. Fischer, “A novel antigen-sampling cell in the teleost gill epithelium with the potential for direct antigen presentation in mucosal tissue,” Frontiers in Immunology, 9, 1-12, 2018
  • [29] C. Lemaitre, N. Orange, P. Saglio, N. Saint, J. Gagnon, and G. Molle, “Characterization and ion channel activities of novel antibacterial proteins from the skin mucosa of carp (Cyprinus carpio),” European Journal of Biochemistry, 240, 143-149, 1996.
  • [30] N. Blackstock and A. D. Pickering, “Acidophilic granular cells in the epidermis of the brown trout, Salmo trutta L.,” Cell and Tissue Research, 210 (3), 359-369, 1980.
  • [31] A. D. Pickering and J. M. Fletcher, “Sacciform cells in the epidermis of the brown trout, Salmo trutta, and the Arctic char, Salvetinus alpinus,” Cell and Tissue Research, 247 (2), 259-265, 1987.

Distribution of Glycoconjugates in the Gills of Oscar Fish (Astronotus ocellatus)

Year 2021, Volume: 16 Issue: 2, 424 - 433, 25.11.2021
https://doi.org/10.29233/sdufeffd.895449

Abstract

Glycoconjugates involved in many functions are produced by many cell types. This study aimed to investigate the glycoconjugate expression in the Oscar fish (Astronotus ocellatus) gills using five horseradish peroxidase-conjugated lectins. This study revealed that gill epithelial cells react with Canavalia ensiformis agglutinin (Con A) and Arachis hypogaea agglutinin (PNA). Strong reaction to Con A was seen in the epithelial cells of the gill primary lamellae. Epithelial cells of primary and secondary lamellae and eosinophilic granular cells had varying intensity of PNA binding. Ulex europaeus agglutinin-I(UEA-I) positive eosinophilic granular cells were observed in the connective tissue of primary lamellae. Epithelial cells of the gill primary filament had Con A reaction in varying intensities. Superficial epithelial cells reacted with PNA in various degrees. Epithelial cells of the secondary lamellae had Con A and PNA positive reaction. These results indicate that glycoconjugate distribution may show interspecific variations, suggesting that the high heterogeneity of naturally occurring glycoconjugates in the epithelial cells of Oscar fish gills might be attributed to the various functional roles of glycoconjugates.

References

  • [1] N. Heisler, “Acid-base regulation in response to changes of the environment characteristics and capacity,” in Fish Ecophysiology, J. C. Rankin, F. B. Jensen, Eds. London: Chapman-Hall, 1993, pp. 207-230.
  • [2] W. S. Hoar and D. J. Randall, “Gills-Anatomy, gas transfer, and acid-base regulation,” in Fish Physiology, vol. XA, New York: Academic Press, 1984, p. 456.
  • [3] W. S. Hoar and D. J. Randall, “Gills-Ion and water transfer,” in Fish Physiology, vol. XB, New York: Academic Press, 1984, p. 416p.
  • [4] E. M. Zuchelkowski, R. C. Lantz, and D. E. Hinton, “Effects of acid-stress on epidermal mucous cells of the brown bullhead Ictalurus nebulosus: A morphometric study,” The Anatomical Record, 200, 33-39, 1981.
  • [5] E. M. Zuchelkowski, C. A. Pinkstaff, and D. E. Hinton, “Mucosubstance histochemistry in control and acid-stressed epidermis of brown bullhead cat fish, Ictalurus nebulosus,” The Anatomical Record, 212, 327-335, 1985.
  • [6] K. L. Shephard, “Functions for fish mucus,” Reviews in Fish Biology and Fisheries, 4, 401-29, 1994.
  • [7] A. Allen, A. Bell, M. Mantle, and J. P. Pearson, “The structure and physiology of gastrointestinal mucus,” Advances in Experimental Medicine and Biology, 144, 115-133, 1982.
  • [8] M. E. V. Johansson, H. Sjövall, and G. C. Hansson, “The gastrointestinal mucus system in health and disease,” Nature Reviews-Gastroenterology and Hepatology, 10, 352-361, 2013.
  • [9] G. J. Strous and J. Dekker, “Mucin-type glycoproteins,” Critical Reviews in Biochemistry and Molecular Biology, 27, 57-92, 1992.
  • [10] F. Genten and A. Danguy, “Light microscopic characterization of glycoconjugates and cell proliferation in teleost epidermis exposed to acute levels of pH,” Zeitschrift fur Mikroskopisch-Anatomische Forschung, 104, 119-139, 1990.
  • [11] S.A. Brooks, “Lectin Histochemistry: Historical Perspectives, State of the Art, and the Future,” Methods in Molecular Biology, 1560, 93-107, 2017.
  • [12] S. M. T. Saboaia‐Moraes, F. J. Hernandez‐Blazquez, D. L. Mota, and A.M. Bittencourt, “Mucous cell types in the branchial epithelium of the euryhaline fish Poecilia vivipara,” Journal of Fish Biology, 49 (3), 545-548. 1996.
  • [13] K. Çınar, N. Şenol, and M. R. Özen, “Histochemical characterization of glycoproteins in the gill of the carp (Cyprinus carpio),” Ankara Üniversitesi Veteriner Fakültesi Dergisi, 55, 61-64, 2008.
  • [14] A. O. Diaz, A. M. Garcia, C. V. Devincenti, and A. L. Goldemberg, “Ultrastructure and histochemical study of glycoconjugates in the gills of the white croaker (Micropogonias furnieri),” Anatomia, Histologia, Embryologia, 34, 117-122, 2005.
  • [15] A. O. Diaz, A. M. Garcia, A. H. Escalante, and A. L. Goldemberg, “Glycoconjugates in the branchial mucous cells of Cynoscion guatucupa,” Scientia Marina, 69 (4), 545-553, 2005.
  • [16] D. M. Fracalossi, M. E. Allen, D. K. Nichols, and O. T. Oftedal, “Oscars, Astronotus ocellatus, have a dietary requirement for vitamin C,” The Journal of Nutrition, 128 (10), 1745-1751, 1998.
  • [17] M. E. de Jesus Trindade, and H. L. de Queiroz, “Feeding ecology and morphometry of the digestive tract of Astronotus ocellatus (Cichlidae) in Várzea environments of the Middle Solimões Region, Central Amazon, Brazil,” Scientific Magazine UAKARI, 8, 45-57, 2012.
  • [18] K. Çınar, M. Öztop, and B. Özkarasu, “Glycoconjugate composition of ovine parotid glands elucidated by lectins,” Journal of Morphological Sciences, 33 (1), 8-13, 2016.
  • [19] M. Öztop, K. Çınar, and E. Demirbağ, “Lectin histochemistry of the glycoconjugates in partridge and quail conjunctival epithelia,” Van Veterinary Journal, 32(1), 1-6, 2021.
  • [20] I. E. Liener, N. Sharon, and I. J. Goldstein, The Lectins: Properties, Functions, and Applications in Biology and Medicine. Orlando, Florida: Academic Press, 1986, p. 618.
  • [21] C. Purbomartono, A. Takemura, and K. Takano, “Histochemical study of mucus lectin distribution on several tissues of tilapia (Oreochromis mossambicus),” Jurnal Perikanan (Journal of Fisheries Sciences), 9 (1), 1-7, 2007.
  • [22] A. O. Díaz, A. M. García, A. H. Escalante, and A. L. Goldemberg, “Glycoproteins histochemistry of the gills of Odontesthes bonariensis (Teleostei, Atherinopsidae),” Journal of Fish Biolology, 77 (7), 1665-1673, 2010.
  • [23] M. C. Rojo, M. J. Blanquez, and M. E. Gonzalez, “A histochemical study of the distribution of lectin binding sites in the developing branchial area of the trout Salmo trutta,” Journal of Anatomy, 189 (3), 609-621, 1996.
  • [24] P. Burkhardt-Holm, “Lectin histochemistry of rainbow trout (Oncorhynchus mykiss) gill and skin,” The Histochemical Journal, 29, 893-899, 1997.
  • [25] G.G. Goss, S. Adamia, and F. Galvez, “Peanut lectin binds to a subpopulation of mitochondria-rich cells in the rainbow trout gill epithelium”, American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 281, 1718-1725, 2001.
  • [26] C. Calabrò, M. P. Albanese, E. R. Lauriano, S. Martella, and A. Licata, “Morphological, histochemical and immunohistochemical study of the gill epithelium in the abyssal teleost fish Coelorhynchus coelorhynchus,” Folia Histochemica et Cytobiologica, 43(1), 51-56, 2005.
  • [27] K. S. Jung, M. J. Ahn, Y. D. Lee, G. M. Go, and T. K. Shin, “Histochemistry of six lectins in the tissues of the flat fish Paralichthys olivaceus,” Journal of Veterinary Science, 3 (4), 292-301, 2002.
  • [28] G. Kato, H. Miyazawa, Y. Nakayama, Y. Ikari, H. Kondo, T. Yamaguchi, M. Sano, and U. Fischer, “A novel antigen-sampling cell in the teleost gill epithelium with the potential for direct antigen presentation in mucosal tissue,” Frontiers in Immunology, 9, 1-12, 2018
  • [29] C. Lemaitre, N. Orange, P. Saglio, N. Saint, J. Gagnon, and G. Molle, “Characterization and ion channel activities of novel antibacterial proteins from the skin mucosa of carp (Cyprinus carpio),” European Journal of Biochemistry, 240, 143-149, 1996.
  • [30] N. Blackstock and A. D. Pickering, “Acidophilic granular cells in the epidermis of the brown trout, Salmo trutta L.,” Cell and Tissue Research, 210 (3), 359-369, 1980.
  • [31] A. D. Pickering and J. M. Fletcher, “Sacciform cells in the epidermis of the brown trout, Salmo trutta, and the Arctic char, Salvetinus alpinus,” Cell and Tissue Research, 247 (2), 259-265, 1987.
There are 31 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Makaleler
Authors

Mustafa Öztop 0000-0002-2923-9280

Kenan Çınar 0000-0003-3410-6180

Emel Demirbağ 0000-0002-8873-4513

Publication Date November 25, 2021
Published in Issue Year 2021 Volume: 16 Issue: 2

Cite

IEEE M. Öztop, K. Çınar, and E. Demirbağ, “Distribution of Glycoconjugates in the Gills of Oscar Fish (Astronotus ocellatus)”, Süleyman Demirel University Faculty of Arts and Science Journal of Science, vol. 16, no. 2, pp. 424–433, 2021, doi: 10.29233/sdufeffd.895449.