Domateste Kök ur nematodu Kontrolünde Ticari Biyonematisit ve Kitosan Kombinasyonları

Abstract

Bu çalışmada Burkholderia rinojensis A396 suşu (Majestene®), Paecilomyces lilacinus 251 suşu (Bio nematon®) ve Quillaja saponaria (QL-Agri®)’dan oluşan üç ticari biyonematisitin kitosan ile kombinasyonlarının domateste kök ur nematodu Meloidogyne incognita’nın oluşturduğu gal ve yumurta paketi üzerindeki etkisi araştırılmıştır. Çalışma plastik saksılarda biyonematisitlerin tekli ve kitosan ile birlikte olacak şekilde 9 uygulaması ile yürütülmüştür. Nematod inokulumu olarak 1000 M. incognita L2/1ml kullanılmış ve nematod inokulasyonu ile birlikte biyonematisit uygulamalarının araziye tavsiye edilen dozları saksı toprağına (Majestene® 0.4 ml/l, Bio nematon® 5 ml/l, Velum Prime® 0.16 ml/L, QL-Agri® 0.8 ml/L) uygulanmıştır. Kitosan uygulaması ise her saksı toprağına %1 ‘lik sıvı süspansiyonundan 5 ml gelecek şekilde yapılmıştır. Altmış gün sonra köklerde 1-9 gal ve yumurta paketi indeks değerine göre değerlendirme gerçekleştirilmiştir. Sadece kitosan uygulamasında gal ve yumurta paketi indeksi sırasıyla 4.2 ve 3.8 saptanmıştır. Sadece biyonematisit uygulamalarında ise gal indeks değeri 3.0-4.8 arasında değişirken, kitosan ile kombinasyonlarında 2.6-3.8 arasında değişim göstermiştir. Sonuçta biyonematisitlerin kitosan ile birlikte uygulamalarının köklerde gallenme ve yumurta paketi sayısını önemli oranda azalttığı ve kök ur nematodu ile mücadelede başarıyı artırdığı saptanmıştır.

Keywords

• Biyokontrol
• Biyonematisit
• Kitosan
• Kök ur nematodu

Combinations of Commercial Bionematicides and Chitosans for Root Knot nematode Control on Tomato

Abstract

In this study, the effect of combination of three commercial bionematicides, Burkholderia rinojensis A396 strain (Majestene®), Paecilomyces lilacinus 251 strain (Bio nematon®) and Quillaja saponaria (QL-Agri®), with chitosan on gall and egg masses formed by root knot nematode Meloidogyne incognita was investigated on tomato under controlled conditions. The study was carried out in plastic pots with 9 applications of bionematicides alone and together with chitosan. A thousand Meloidogyne incognita second stage juvenile (L2)/1ml was used as nematode inoculum and the recommended doses of bionematicide applications to the field (Majestene® 0.4 ml/l, Bio nematon® 5 ml/l, Velum Prime® 0.16 ml/L, QL- Agri® 0.8 ml/L) with nematode inoculation were applied to the potting soil. Chitosan application was made 5 ml of 1% liquid suspension required for each potting soil. After sixty days, the roots were evaluated according to the index value of 1-9 gall and egg mass. The gall and egg mass index were found to be 4.2 and 3.8, respectively in only chitosan application. While the gall index changed between 3.0-4.8 in only bionematicide applications, it changed between 2.6-3.8 in combination with chitosan. As a result, the application of bionematicides together with chitosan significantly reduced the number of galls and egg masses in the roots of tomato and increased the success in the control against M. incognita.

Keywords

• Biocontrol
• Bionematicide
• Chitosan
• Root knot nematode

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