Kuersetin ve sabinenin model böcek Drosophila melanogaster'in (Diptera: Drosophilidae) antioksidan ve detoksifikasyon enzimleri üzerindeki etkileri: Moleküler kenetlenme araştırması

Abstract

Lepidoptera ve Diptera takımındaki tarım zararlısı böcekler ürün ve ekonomik kayıplara yol açar. Kimyasal böcek öldürücüler giderek daha yaygın hale geldikçe, hedef dışı türler ve çevre üzerindeki zararlı etkilerine ilişkin endişeler artmakta ve daha az toksik alternatif kimyasallara ihtiyaç duyulmaktadır. Bu çalışmada, biyolojik araştırmalarda model organizma olarak kullanılan Drosophila melanogaster'de, iki doğal bileşik olan kuersetin ve sabinenin, önemli antioksidan ve detoksifikasyon enzimleriyle moleküler etkileşimleri araştırılmaktadır. Bu çalışmada, kuersetin ve sabinenin sitokrom P450 (CYP450), glutatyon S-transferaz (GST), süperoksit dismutaz (SOD), katalaz (CAT) ve glutamat-sistein ligaz (GCL) gibi enzimlerle bağlanma afiniteleri moleküler yerleştirme teknikleri kullanılarak değerlendirilmiştir. Sonuçlar, kuersetin'in sabinene kıyasla tüm enzimlerle daha güçlü etkileşimler sergilediğini, kuersetin ve CAT (-10,7 kcal/mol) arasında en yüksek bağlanma enerjisinin gözlemlendiğini ortaya koydu. Bu bulgular, kuersetin'in D. melanogaster'in antioksidan ve detoksifikasyon sistemlerini önemli ölçüde etkilediğini ve potansiyel olarak oksidatif stresi artırdığını göstermektedir. Sabinen, tüm enzimlerde daha zayıf bağlanma afinitesi gösterdi ve bu da daha az etki olduğunu göstermektedir. Bu çalışma, kuersetin ve sabinenin zararlı kontrol stratejilerinde ajan olarak potansiyeline ilişkin önemli sonuçlar sunmaktadır.

Keywords

• Model böcek
• detoksifikasyon
• moleküler yerleştirme
• kuersetin
• antioksidan enzimler

Effects of quercetin and sabinene on antioxidant and detoxification enzymes of model pest Drosophila melanogaster (Diptera: Drosophilidae): Molecular docking investigation

Abstract

Agricultural pest insects, particularly those within the Lepidoptera and Diptera orders, are responsible for significant crop damage, leading to economic losses. As chemical insecticides become increasingly prevalent, concerns over their detrimental impact on non-target species and the environment grow, emphasizing the need for less toxic alternatives. This study investigates the molecular interactions of quercetin and sabinene - two naturally occurring compounds - with key antioxidant and detoxification enzymes in Drosophila melanogaster, a model organism in biological research. In this study, the binding affinities of quercetin and sabinene with enzymes such as cytochrome P450 (CYP450), glutathione S-transferase (GST), superoxide dismutase (SOD), catalase (CAT) and glutamate-cysteine ligase (GCL) were evaluated using molecular docking techniques. The results revealed that quercetin exhibits stronger interactions with all enzymes compared to sabinene, with the highest binding energy observed between quercetin and CAT (-10.7 kcal/mol). These findings suggest that quercetin significantly affects the antioxidant and detoxification systems of D. melanogaster, potentially enhancing oxidative stress. Sabinene demonstrated weaker binding across all enzymes, indicating a lesser impact. The study contributes valuable insights into the potential of quercetin and sabinene as agents in pest control strategies by targeting insect biochemical pathways.

Keywords

• Model insect
• detoxification
• molecular docking
• quercetin
• antioxidant enzymes

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