Koyun (Ovis aries) Fundus ve Pilorus Mukozalarındaki Şekerlerin Lektin Histokimyasal Karakterizasyonu

Abstract

Bu çalışmada koyun (Ovis aries) abomazumunun fundus ve pilorus bölgelerinde bulunan şekerlerin Bandeiraea simplicifolia (BSI-B4) ve Triticum vulgare (WGA) lektinlerine karşı verdikleri reaksiyonların belirlenmesi amaçlandı. İncelenen her iki bölgede BSI-B4 ve WGA uygulamalarına karşı lamina epitelyalis yüzeyinin çok yoğun reaksiyon gösterdiği saptandı. Ayrıca prensipal hücrelerde BSI-B4 reaksiyonunun çok yoğun, WGA reaksiyonunun ise yoğun olduğu tespit edildi. WGA uygulamasında bütün parietal hücrelerde orta derecede reaksiyona rastlanırken, BSI-B4 uygulamasında bazı parietal hücrelerde çok yoğun, bazılarında ise orta yoğunlukta reaksiyon belirlendi. Pilorus bezlerinin yüzeye yakın kısımlarındaki hücrelerde yoğun WGA ve BSI-B4, dip kısımlarındaki hücrelerde ise orta derecede WGA ve zayıf BSI-B4 reaktivitesi gözlendi.

Keywords

• Lektin Histokimyası
• Abomazum
• Musin
• WGA
• BSI-B4

Lectin Histochemical Characterization of Sugars in Mucozas of Fundus and Pylorus of Sheep (Ovis aries)

Abstract

In this study it was aimed to determinate the reactions of sugars in fundus and pylorus regions of sheep (Ovis aries) abomasum against to Bandeiraea simplicifolia (BSI-B4) and Triticum vulgare (WGA) lectins. In the both investigated regions it was identified that the surface of lamina epithelialis showed very dense reaction against to BSI-B4 and WGA stainings. Also it was
determined the BSI-B4 reaction was very intense and WGA reaction was intense in the prencipal cells. While there were moderate reaction in all parietal cells by WGA staining, very dense reaction in some parietal cells and moderate reaction in the others by BSIB4 staining. It was observed that dense WGA and BSI-B4 reactivities were in the cells near the surface region of pylorus glands, moderate WGA and weak BSI-B4 reactivities were in the cells bottom parts of pylorus glands.

Keywords

• Lectin Histochemistry
• Abomasum
• Mucin
• WGA
• BSI-B4

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