Bazı Ticari Kitler Kullanılarak Et Ürünlerindeki Tavuk ve Sığır Eti Miktarının Belirlenmesi

Abstract

Hayvansal gıda kaynakları insan beslenmesinde hayati önem taşımaktadır. Dengeli bir diyet, yeterli ve kaliteli gıda maddeleri içermelidir. Uygun etiketleme insan sağlığı için önemli olduğu kadar et endüstrisinde adil ticaret için de önemlidir. DNA, fiziksel ve kimyasal gıda işleme tekniklerinden etkilenmediği için, et ürünlerini oluşturan içeriğin kaynağı olan türleri ve oranlarını belirlemek amacıyla DNA tabanlı kitler üretilmiştir. Çalışmamızın amacı, ticari kitlerin Türkiye’deki et ürünleri tebliğine uygunluğunu incelemek ve et ürünlerindeki tavuk ve sığır eti miktarını belirlemesini araştırmaktır. Çalışmamızda et ürünlerindeki tavuk ve sığır eti miktarının belirlemek için TaqMan prob kullanan Real Time PCR kitleri kullanılmıştır. Çalışma sonunda incelenen kitlerin RT-PCR reaksiyonlarının eğim ve korelasyon katsayısı kriterleri uygun bulunmuş ancak et ürünlerindeki çeşitli oranlardaki tavuk ve sığır etlerinin kantitatif tayinleri yeterli bulunmamıştır.    

Keywords

• Et ürünleri,Sığır eti,Tavuk eti,Miktar tayini,Real time PCR

Determination The Proportion of Chicken and Beef In Meat Products Using Some Commercial Kits

Abstract

Animal food sources are vital to human nutrition. A balanced diet should contain adequate and high-quality food items. Proper labeling is important for human health as well as for fair trade in meat industry. Since DNA is not affected by physical and chemical food processing techniques, DNA-based kits are produced to determine species and their proportions in meat products. The aim of our study was to examine the suitability of commercially available kits for Turkey notified meat products and to investigate proportion of chicken and beef in meat products. Real Time PCR kits using TaqMan probe were used to determine the amount of chicken and beef in meat products. As a result of the study, the slope and correlation coefficient criteria of the RT-PCR reactions of the kit were appropriate, but sensitivity of quantitative determinations of different chicken and beef meat products were not sufficient.    

Keywords

• Meat products,Beef,Chicken meat,Quantitation,Real time PCR

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