Bazı Ticari Kitler Kullanılarak Et Ürünlerindeki Tavuk ve Sığır Eti Miktarının Belirlenmesi

Yıl 2019, , 52 - 58, 01.04.2019

Muhammet Kaya , Ayşegül Arıkan Asan Şafak Başıaçık Karakoç

https://doi.org/10.19113/sdufenbed.442199

Cited By: 1

Öz

Hayvansal
gıda kaynakları insan beslenmesinde hayati önem taşımaktadır. Dengeli bir
diyet, yeterli ve kaliteli gıda maddeleri içermelidir. Uygun etiketleme insan
sağlığı için önemli olduğu kadar et endüstrisinde adil ticaret için de
önemlidir. DNA, fiziksel ve kimyasal gıda işleme tekniklerinden etkilenmediği
için, et ürünlerini oluşturan içeriğin kaynağı olan türleri ve oranlarını
belirlemek amacıyla DNA tabanlı kitler üretilmiştir. Çalışmamızın amacı, ticari
kitlerin Türkiye’deki et ürünleri tebliğine uygunluğunu incelemek ve et
ürünlerindeki tavuk ve sığır eti miktarını belirlemesini araştırmaktır. Çalışmamızda
et ürünlerindeki tavuk ve sığır eti miktarının belirlemek için TaqMan prob
kullanan Real Time PCR kitleri kullanılmıştır. Çalışma sonunda incelenen
kitlerin RT-PCR reaksiyonlarının eğim ve korelasyon katsayısı kriterleri uygun
bulunmuş ancak et ürünlerindeki çeşitli oranlardaki tavuk ve sığır etlerinin
kantitatif tayinleri yeterli bulunmamıştır.    

Anahtar Kelimeler

Et ürünleri, Sığır eti, Tavuk eti, Miktar tayini, Real time PCR

Determination The Proportion of Chicken and Beef In Meat Products Using Some Commercial Kits

Öz

Animal
food sources are vital to human nutrition. A balanced diet should contain
adequate and high-quality food items. Proper labeling is important for human
health as well as for fair trade in meat industry. Since DNA is not affected by
physical and chemical food processing techniques, DNA-based kits are produced
to determine species and their proportions in meat products. The aim of our
study was to examine the suitability of commercially available kits for Turkey
notified meat products and to investigate proportion of chicken and beef in
meat products. Real Time PCR kits using TaqMan probe were used to determine the
amount of chicken and beef in meat products. As a result of the study, the
slope and correlation coefficient criteria of the RT-PCR reactions of the kit
were appropriate, but sensitivity of quantitative determinations of different
chicken and beef meat products were not sufficient.    

Anahtar Kelimeler

Meat products, Beef, Chicken meat, Quantitation, Real time PCR

Kaynakça

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