Koyun (Ovis aries) Trakea Mukozasındaki Bazı Peptidlerin İmmunohistokimyasal Lokalizasyonları

Ruaa Hussein Nada; Kenan Çınar

doi:10.19113/sdufbed.47000

Koyun (Ovis aries) Trakea Mukozasındaki Bazı Peptidlerin İmmunohistokimyasal Lokalizasyonları

Abstract

Bu çalışmada, koyun (Ovis aries), (alt ve üst) trake mukozasında GAL (Galanin), PHI (Peptit Histidin İzolösin) ve Leu-Enk (Leu-Enkefalin) peptitlerinin immunohistokimyasal lokalizasyonlarının belirlenmesi amaçlandı. Üst trake lamina epitelyalisindeki GAL, PHI ve Leu-ENK immunoreaktif hücrelerin yoğunluğunun alt trakeye göre daha fazla olduğu saptandı. PHI immunoreaktif hücrelerin ise üst trake lamina epitelyalisinde diğer peptitlere göre daha fazla sayıda bulunduğu belirlendi. GAL, PHI ve Leu-ENK immunoreaktivitesine aynı zamanda düz kas, serömuköz bez hücreleri, bu bezler ile kan damarları çevresinde ve gangliyon hücrelerinde de rastlandı. Sonuç olarak GAL, Leu-ENK ve PHI’nın geniş mukozal dağılımının, memeli solunum sistemindeki vasküler ve sekretuvar fonksiyonların düzenlemesinde etkili olabileceği düşünülmektedir. Solunum yolu epitellerinde bulunan GAL, PHI ve ENK peptidleri, düzenleyici peptid hormonların ya da nörotransmitterlerin lokal olarak harekete geçirilmesinde de etkili olabilirler.

Keywords

Trakea,Mukoza; İmmunohistokimya; Galanin; Peptide Histidin İzolösin; Leu-Enkefalin

References

[1] Lauweryns, J.M., Van Lommel, A.T., Dom, R.J. 1985. Innervation of Rabbit Intrapulmonary Neuroepithelial Bodies. Quantitative and Qualitative Ultrastructural Study after Vagotomy. Journal of the Neurological Sciences, 67, 81-92.
[2] Van Lommel, A., Bolle, T., Fannes, W. Lauweryns, J.M., 1999. The Pulmonary Neuroendocrine System: the Past Decade. Archives of Histology and Cytology, 62(1), 1-16.
[3] Van Lommel, A., 2001. Pulmonary Neuroendocrine Cells (PNEC) and Neuroepithelial Bodies (NEB): Chemoreceptors and Regulators of Lung Development. Paediatric Respiratory Reviews, 2(2),171-176.
[4] Salvi, E.P., Vaccaro, R., 2001. Ontogeny of Galanin-Immunoreactive Elements in Chicken Embryo Autonomic Nervous System. Anatomical Record, 262(3), 266-278.
[5] Mitsukawa, K., Lu, X., 2008. Galanin, Galanin Receptors and Drug Targets. Cellular and Molecular Life Sciences, 65(12), 1796-1805.
[6] Counts, S.E., Perez S.E., Kahl, U., Bartfai, T., Bowser, R.P., Deecher, D.C., Mash, D.C., Crawley, J.N., Mufson, E.J., 2001. Galanin: Neurobiologic Mechanisms and Therapeutic Potential for Alzheimer’s Disease. CNS Drug Reviews, 7, 445–470.
[7] Cheung, A., Polak, J.M., Bauer, F.E., Cadieux, A., Christofides, N.D., Springall, D.R., Bloom, S.R., 1985. Distribution of Galanin Immunoreactivity in the Respiratory Tract of Pig, Guinea Pig, Rat, and Dog. Thorax, 40(12), 889-896.
[8] Palle, C., Ottesen, B., Jorgensen, J., Fahrenkrug, J., 1989. Peptide Histidine Methionine and Vasoactive Intestinal Peptide: Occurrence and Relaxant Effect in the Human Female Reproductive Tract. Biology of Reproduction, 41, 1103–1111.

[9] Bailey, C.J., Wilkes, L.C., Conlon, J.M., Armstrong, P.H., Buchanan, K.D., 1990. Effects of Gastric Inhibitory Polypeptide, Vasoactive Intestinal Polypeptide and Peptide Histidine Isoleucine on the Secretion of Hormones by Isolated Mouse Pancreatic Islets. The Journal of Endocrinology, 125, 375–379.
[10] Mikkelsen, J.D, Fahrenkrug, J., 1994. Concentrations and Distribution of Vasoactive Intestinal Peptide (VIP), Peptide Histidine Isoleucine (PHI) and Peptide Histidine Valine (PHV) in the Cerebral Cortex and the Suprachiasmatic Nucleus of the Mouse. Brain Research, 656, 95–107.
[11] Skakkeboek, M.L., Georg, B., Mikkelsen, J.D., Ottesen, B., Fahrenkrug, J. 1995. All Prepro-VIP-Derived Peptides, except PHI/PHV, Are Expressed in the Female Rat Anterior Pituitary and Increased by Estrogen. Peptides 7, 1287-1294.
[12] Buhl, T., Nilsson, C., Ekblad, E., Johnsen, A.H., Fahrenkrug, J., 1996. Expression of Prepro-VIP Derived Peptides in the Gastrointestinal Tract of Normal, Hypothyroid and Hyperthyroid Rats. Neuropeptides, 30, 237–247.
[13] Lundberg, J. M., Fahrenkrug, J., Hökfelt, T., Martling, C. R., Larsson, O., Tatemoto, K., Anggård, A., 1984. Co-existence of Peptide HI (PHI) and VIP in Nerves Regulating Blood Flow and Bronchial Smooth Muscle Tone in Various Mammals Including Man. Peptides, 5(3), 593-606.
[14] Christofides, N.D., Yiangou, Y., Piper, P.J., Ghatei, M.A., Sheppard, M.N., Tatemoto, K., Bloom, S.R., 1984. Distribution of Peptide Histidine Isoleucine in the Mammalian Respiratory Tract and Some Aspects of Its Pharmacology. Endocrinology, 115(5), 1958-1963.
[15] Ishizuka, T., Kurosawa, M., 1990. Basic Study of Peptide Histidine Isoleucine (PHI) Immunoreactivity in the Rat Respiratory Tract. Arerugi, 39(7), 577-586.
[16] Greenberg, B., Rhoden, K., Barnes, P.J., 1987. Vasoactive Intestinal Peptide Causes Non-Endothelial Dependent Relaxation in Human and Bovine Pulmonary Arteries. Blood Vessels, 24, 45-50.
[17] Said, S.I., 1982. Vasoactive Peptides in the Lung, with Special Reference to Vasoactive Intestinal Peptide. Experimental Lung Research, 3(3-4), 343-348.
[18] Tatemoto, K., Mutt, V., 1981. Isolation and Characterization of the Intestinal Peptide Porcine PHI (PHI-27), A New Member of the Glucagon–Secretin Family. Proceedings of the National Academy of Sciences, 78(11), 6603–6607.
[19] Roth, K.A., Barchas, J.D., 1986. Small Cell Carcinoma Cell Lines Contain Opioid Peptides and receptors. Cancer, 57(4), 769-773.
[20] Maneckjee, R., Minna, J.D., 1990. Opioid and Nicotine Receptors Affect Growth Regulation of Human Lung Cancer Cell Lines. Proceedings of the National Academy of Sciences, 87(9), 3294-3298.
[21] Cutz, E., Chan, W., Track, N.S., 1981. Bombesin, Calcitonin and Leu-enkephalin Immunoreactivity in Endocrine Cells of Human Lung. Experientia, 37(7), 765-767.
[22] Zaccone, G., Mauceri, A., Cascio, P.L., Minniti, F., Parrino, V., Fasulo, S., 2004. Immunohistochemical Study of the Innervation of Pulmonary Vessels and Smooth Muscles in the Respiratory Tract of Two Frog Species. Acta Histochemica, 106(3), 179-193.
[23] Sternberger, L.A., 1989. Immunocytochemistry. John Wiley and Sons, 524p. New York.
[24] Porzionato, A., Macchi, V., Barzon, L., Masi, G., Belloni, A., Parenti, A., Palù, G., De Caro, R., 2010. Expression and Distribution of Galanin Receptor Subtypes in the Rat Carotid Body. Molecular Medicine Reports, 3(1), 37–41.
[25] Dey, R.D., Zhu, W., 1993. Origin of Galanin in Nerves of Cat Airways and Colocalization with Vasoactive Intestinal Peptide. Cell and Tissue Research, 273(1), 193-200.
[26] Kusindarta, D.L., Wijayanto, H., Atoji, Y., 2003. Intrinsic Innervation in the Tracheal Smooth Muscle of the Large Flying Fox (Pteropus vampyrus): An Immunohistochemical Study. European Journal of Mmorphology, 41(3/4), 111-116.
[27] Ghatei, M.A., Springall, D.R., Richards, I.M., Oostveen, J.A., Griffin, R.L., Cadieux, A., Bloom, S.R., 1987. Regulatory Peptides in the Respiratory Tract of Macaca fascicularis. Thorax, 42(6), 431-439.
[28] Luts, A., Uddman, R., Sundler, F., 1989. Neuronal Galanin is Widely Distributed in the Chicken Respiratory Tract and Coexists with Multiple Neuropeptides. Cell and Tissue Research, 256, 95–103.
[29] Yamamoto, Y., Ootsuka, T., Atoji, Y., Suzuki, Y., 2000. Tyrosine Hydroxylase-and Neuropeptides-Immunoreactive Nerves in Canine Trachea. American Journal of Veterinary Research, 61(11), 1380-1383.
[30] Luts, A., Sundler, F., 1989. Peptide-Containing Nerve Fibers in the Respiratory Tract of the Ferret. Cell and Tissue Research, 258, 259-267.
[31] Polak, J.M., Bloom, S.R., 1984. Regulatory Peptides—the Distribution of Two Newly Discovered Peptides: PHI and NPY. Peptides, 5, 79-89.
[32] Uddman, R., Luts, A., Sundler, F., 1985. Nerve Fibres Containing Peptide Histidine Isoleucine (PHI) in the Respiratory Tract. Archives of Otorhinolaryngology, 242(2), 189-193.
[33] Martling, C.R., Matran, R., Alving, K., Hökfelt, T., & Lundberg, J. M., 1990. Innervation of lower airways and neuropeptide effects on bronchial and vascular tone in the pig. Cell and tissue research, 260(2), 223-233.
[34] Corbett, A.D., Paterson, S.J., Kosterlitz, H.W., 1993. Selectivity of Ligands for Opioid Receptors. In Herz, A., Akil, H., Simon, E.J. (Ed.), Opioids (645-679), Springer, 815p. Berlin Heidelberg.
[35] Beorlegui, C., Sesma, P., Martı́nez, A., 1994. An Immunocytochemical Study of the Respiratory System of Podarcis hispanica (Reptilia). General and Comparative Endocrinology, 96(3), 327-338.
[36] Shimosegawa, T., Foda, H.D., Said, S.I., 1989. Immunohistochemical Demonstration of Enkephalin-Containing Nerve Fibers in Guinea Pig and Rat Lungs. The American Review of Respiratory Disease, 140(426), 441-448.

Details

Primary Language

Turkish

Subjects

-

Journal Section

-

Authors

Ruaa Hussein Nada This is me

Kenan Çınar

Publication Date

August 15, 2018

Submission Date

March 21, 2017

Acceptance Date

-

Published in Issue

Year 2018 Volume: 22 Number: 2

DOI

https://doi.org/10.19113/sdufbed.47000

IZ

https://izlik.org/JA63FH66GY

Cite

RIS / Bibtex

APA

Nada, R. H., & Çınar, K. (2018). Koyun (Ovis aries) Trakea Mukozasındaki Bazı Peptidlerin İmmunohistokimyasal Lokalizasyonları. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 22(2), 855-860. https://doi.org/10.19113/sdufbed.47000

AMA

1.Nada RH, Çınar K. Koyun (Ovis aries) Trakea Mukozasındaki Bazı Peptidlerin İmmunohistokimyasal Lokalizasyonları. J. Nat. Appl. Sci. 2018;22(2):855-860. doi:10.19113/sdufbed.47000

Chicago

Nada, Ruaa Hussein, and Kenan Çınar. 2018. “Koyun (Ovis Aries) Trakea Mukozasındaki Bazı Peptidlerin İmmunohistokimyasal Lokalizasyonları”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 22 (2): 855-60. https://doi.org/10.19113/sdufbed.47000.

EndNote

Nada RH, Çınar K (August 1, 2018) Koyun (Ovis aries) Trakea Mukozasındaki Bazı Peptidlerin İmmunohistokimyasal Lokalizasyonları. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 22 2 855–860.

IEEE

[1]R. H. Nada and K. Çınar, “Koyun (Ovis aries) Trakea Mukozasındaki Bazı Peptidlerin İmmunohistokimyasal Lokalizasyonları”, J. Nat. Appl. Sci., vol. 22, no. 2, pp. 855–860, Aug. 2018, doi: 10.19113/sdufbed.47000.

ISNAD

Nada, Ruaa Hussein - Çınar, Kenan. “Koyun (Ovis Aries) Trakea Mukozasındaki Bazı Peptidlerin İmmunohistokimyasal Lokalizasyonları”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 22/2 (August 1, 2018): 855-860. https://doi.org/10.19113/sdufbed.47000.

JAMA

1.Nada RH, Çınar K. Koyun (Ovis aries) Trakea Mukozasındaki Bazı Peptidlerin İmmunohistokimyasal Lokalizasyonları. J. Nat. Appl. Sci. 2018;22:855–860.

MLA

Nada, Ruaa Hussein, and Kenan Çınar. “Koyun (Ovis Aries) Trakea Mukozasındaki Bazı Peptidlerin İmmunohistokimyasal Lokalizasyonları”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol. 22, no. 2, Aug. 2018, pp. 855-60, doi:10.19113/sdufbed.47000.

Vancouver

1.Ruaa Hussein Nada, Kenan Çınar. Koyun (Ovis aries) Trakea Mukozasındaki Bazı Peptidlerin İmmunohistokimyasal Lokalizasyonları. J. Nat. Appl. Sci. 2018 Aug. 1;22(2):855-60. doi:10.19113/sdufbed.47000