Determination of Bean yellow mosaic virus (BYMV) in Common Bean Plants from Burdur Province

Abstract

In this study, the infection of Bean yellow mosaic virus (BYMV) was investigated in Burdur province which has a significant contribution bean production of Turkey. Firstly, surveys were conducted in bean production areas and leaf samples showing virus like symptoms were collected in the region. The presence of BYMV in the collected bean leaves was analyzed by biological and serological methods. A total 443 samples were collected from field and all samples were tested for BYMV using spesific antiserum in a double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA). As a result of DAS-ELISA, 97 out of 443 samples (21.89%) were infected with BYMV. DAS-ELISA positive samples were later inoculated into indicator plants and their symptoms observed. As a result of the study, BYMV infections were detected in the leaf samples in bean growing areas in Burdur province.

Keywords

• Bean,Bean yellow mosaic virus,Detection

Burdur İlinde Yetiştirilen Fasulye Bitkilerinde Bean Yellow Mosaic Virus (BYMV)’ünün Araştırılması

Abstract

Bu çalışmada Türkiye’deki fasulye yetiştiriciliğinde önemli bir paya sahip olan Burdur ilinde BYMV (Bean yellow mosaic virus)’ün varlığı araştırılmıştır. Öncelikli olarak bu bölgedeki fasulye üretim alanlarına sürveyler yapılarak virüs simptomu sergileyen fasulye yaprak örnekleri alınmıştır. Toplanan fasulye yaprak örneklerinde BYMV’nin varlığı biyolojik ve serolojik olarak araştırılmıştır. Arazi çalışmaları sonucunda; 443 örnek toplanmış ve bütün örnekler BYMV’ne spesifik antiserumlar kullanılarak double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) yöntemiyle testlenmiştir. DAS-ELISA testi sonucunda 443 örnekten 97’sinde (% 21.89) BYMV belirlenmiştir. DAS-ELISA testi sonucunda pozitif reaksiyon veren yaprak örnekleri, test bitkilerine inokule edilmiş ve oluşan belirtiler değerlendirilmiştir. Çalışmaların sonucunda Burdur fasulye üretim alanlarından alınan yaprak örneklerinde BYMV enfeksiyonları saptanmıştır.

Keywords

• Fasulye,Bean yellow mosaic virus,Teşhis

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