Determination of Bean yellow mosaic virus (BYMV) in Common Bean Plants from Burdur Province

Öz

In this study, the infection of Bean yellow mosaic virus (BYMV) was investigated in Burdur province which has a significant contribution bean production of Turkey. Firstly, surveys were conducted in bean production areas and leaf samples showing virus like symptoms were collected in the region. The presence of BYMV in the collected bean leaves was analyzed by biological and serological methods. A total 443 samples were collected from field and all samples were tested for BYMV using spesific antiserum in a double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA). As a result of DAS-ELISA, 97 out of 443 samples (21.89%) were infected with BYMV. DAS-ELISA positive samples were later inoculated into indicator plants and their symptoms observed. As a result of the study, BYMV infections were detected in the leaf samples in bean growing areas in Burdur province.

Anahtar Kelimeler

• Bean,Bean yellow mosaic virus,Detection

Burdur İlinde Yetiştirilen Fasulye Bitkilerinde Bean Yellow Mosaic Virus (BYMV)’ünün Araştırılması

Öz

Bu çalışmada Türkiye’deki fasulye yetiştiriciliğinde önemli bir paya sahip olan Burdur ilinde BYMV (Bean yellow mosaic virus)’ün varlığı araştırılmıştır. Öncelikli olarak bu bölgedeki fasulye üretim alanlarına sürveyler yapılarak virüs simptomu sergileyen fasulye yaprak örnekleri alınmıştır. Toplanan fasulye yaprak örneklerinde BYMV’nin varlığı biyolojik ve serolojik olarak araştırılmıştır. Arazi çalışmaları sonucunda; 443 örnek toplanmış ve bütün örnekler BYMV’ne spesifik antiserumlar kullanılarak double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) yöntemiyle testlenmiştir. DAS-ELISA testi sonucunda 443 örnekten 97’sinde (% 21.89) BYMV belirlenmiştir. DAS-ELISA testi sonucunda pozitif reaksiyon veren yaprak örnekleri, test bitkilerine inokule edilmiş ve oluşan belirtiler değerlendirilmiştir. Çalışmaların sonucunda Burdur fasulye üretim alanlarından alınan yaprak örneklerinde BYMV enfeksiyonları saptanmıştır.

Anahtar Kelimeler

• Fasulye,Bean yellow mosaic virus,Teşhis

Kaynakça

1. [1] Singh, S. P. 1999. Integrated Genetic Improvement. In: Common Bean Improvement in The Twenty-First Century. Kluwer Academic Publishers, Dordrecht, The Netherlands, 133-165s.
2. [2] FAO, 2017. Food and Agriculture Organization of the United Nations. http://www. fao.org/faostat (Erişim Tarihi: 14.02.2018).
3. [3] Aydoğan M., Demiryürek, K., Abacı, N. İ. 2015. Türkiye’de Kuru Fasulye Üretiminin Mevcut Durumu ve Gelecek Dönemler Üretiminin Tahmin Edilmesi. Türk Tarım–Gıda Bilim ve Teknoloji Dergisi, 3(12), 962-968.
4. [4] Agrios, G. N. 1997. Plant Diseases Caused by Viruses. Plant Pathology, 479-556s.
5. [5] Loebenstein, G., Carr, J. P. 2006. Natural Resistance Mechanisms of Plants to Viruses. Springer, the Netherlands, 367-382.
6. [6] Hosseini, A., Hosseini, S. 2014. Occurrence and Distribution of Bean Common Mosaic Virus and Bean Yellow Mosaic Virus from Common Bean Fields of Kerman Province, Iran. Indian Journal of Fundamental and Applied Life Sciences, 4(2), 528-535.
7. [7] Al-Ani, R. A., Adhab, M. A. 2013. Bean Yellow mosaic virus (BYMV) on Broadbean; Characterization and Resistance Induced by Rhizobium Leguminuarum. Journal of Pure and Applied Microbiology, 7(1), 135-142.
8. [8] Çulal-Kılıç, H., Yardımcı, N. 2012. Burdur Çine Ovası Fasulye Alanlarında Hıyar Mozaik Virüsü. Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 3(2), 12-15.

9. [9] Çulal-Kılıç, H., Yardımcı, N. 2014. Burdur İli Fasulye Üretim Alanlarında Fasulye Adi Mozayik Virüsü’nün Serolojik Ve Moleküler Yöntemlerle Belirlenmesi. Türk Tarım ve Doğa Bilimleri Dergisi 1(2), 289–294.
10. [10] Clark, M. F., Adams, A. N. 1977. Characteristics of the Microplate Method of Enzyme-Linked Immunosorbent Assay for the detection of plant viruses. Journal of General Virology, 34, 475-483.
11. [11] Sharma P. N., Sharma, V., Sharma, A., Rajput, K., Sharma, S. K.‎ 2015. Identification and Molecular Characterization of Bean Yellow Mosaic Virus İnfecting French Bean in Himachal Pradesh. Virus disease, 26(4), 315-8.
12. [12] Kutluk Yılmaz, N. D., Gümüş, M., Erkan, S. 2002. Tokat İlinde Fasulye Tohumlarındaki Viral Etmenlerin Saptanması Üzerinde Araştırmalar. Ege Üniversitesi, Ziraat Fakültesi Dergisi, 39(3), 49-55.
13. [13] Güzel, Ö., Arlı-Sökmen, M. 2003. Determination of Some Viruses Infecting Common Bean (Phaseoulus Vulgaris L.) and their Incidences in Seed Lots in Samsun Province. Journal of Turkish Phytopathology, 32( 2), 99-106.
14. [14] Çulal-Kılıç, H., Yardımcı, N., Açıkyürek, S., Uzal, A. 2015. Detection of BCMV, AMV and CMV By DAS-ELISA and Immunocapture-RT-PCR in Bean-Growing Areas in The West Mediterranean Region, Turkey. Fresenius Environmental Bulletin, 24(5), 1752-1756.
15. [15] Melgarejo T. A., Lehtonen, M. T. , Fribourg, C., E. , Rannali, M., Valkonen, J. P. T. 2007. Strains of BCMV and BCMNV Characterized from Lima Bean Plants Affected by Deforming Mosaic Disease in Peru. Archives of Virology, 152, 1941–1949.
16. [16] Abtahi, F. S., Habibi, M. K., Motlagh, M. K. 2009. Some Biological and Molecular Characterization of Bean Common Mosaic Necrosis Virus İsolated from Soybean in Tahran Province, Iran. World Academy of Science, Engineering and Techology, 49, 126-129.
17. [17] Pudashini, B. J., Shahid, M. S., Natsuaki, K. T. 2013. First Report of Bean Common Mosaic Necrosis Virus (BCMNV) Infecting Sweet Bean in Nepal. Plant Disease, 97(2), 290-290.
18. [18] Duraisamy, G. S., Pokorný, R., Holková, L. 2011. Possibility of Bean Yellow Mosaic Virus Detection in Gladiolus Plants by Different Methods. Journal of Plant Diseases and Protection, 118(1), 2–6.
19. [19] Campos, R. E., Bejerman, N., Nome, C., Laguna, I. G. Rodriguez, P. P. 2013. Bean Yellow Mosaic Virus in Soybean from Argentina. Journal of Phytopathology, 162, 322–325.
20. [20] Ghorbani S. G. M., Shahraeena, N., Elahinia S. A. 2010. Distribution and impact of virus associated diseases of common bean (Phaseolus vulgaris L.) in Northern Iran. Archives of Phytopathology and Plant Protection, 43(12), 1183–1189.