Sentetik Atıksu İçerisindeki Borodinellopsis texensis CCALA 892’den Biyokütle ve Biyo-bütanol Üretimi: Biyokimyasal Kompozisyonun Belirlenmesi

Abstract

Mikroalgler genelde biyokütlenin yüksek miktarlarını üretebilirler ve biyokütleden biyo-bütanole dönüştürülebilirler. Biyo-bütanol sıvı bir yakıttır ve önemli fiziksel ve kimyasal özelliklere sahiptir. Bu çalışmada, belediye atık suyunun çeşitli konsantrasyonunda büyütülen Borodinellopsis texensis CCALA 892’den biyo-bütanol üretimini inceledik. Birde, mikroalg örneklerinin biyokimyasal içeriğinin oranlarını belirleyerek katalaz, süperoksit dismutaz ve askorbat peroksidaz gibi bazı antioksidan enzimleri çalıştık. Yaygın çalışmada, biyo-bütanol aseton-bütanol-etanol (ABE) fermantasyon metodu ile üretildi. Beş atık su örneği içerisinde %25 atıksu içerisinde büyütülen mikroalg örneği 0,114 ± 0,002 g L-1g-1 ile en yüksek biyokütle verimine sahipti. Kontrol grubunun karbonhidrat ve protein konsantrasyonları gün ve gün arttı ve değerleri yedi günde durağan faza ulaştı. Durağan fazda, %25 atık su içerisinde büyütülen mikroalg örneği 0,30 g L-1 ile en yüksek karbonhidrat konsantrasyonu ve 0,35 g L-1 ile de en yüksek protein konsantrasyonuna sahipti. %25 atık su içerisindeki mikroalg örneğinde katalaz, superoksit dismutaz ve askorbat peroksidaz’ın maksimum enzim aktiviteleri sırası ile 15,33 ± 0,88, 8,67 ± 0,67 and 33 ± 1,53 µmole/mg idi. Buna ek olarak, B. texensis CCALA 892’nin biyo-bütanol içeriği 3,63 ± 0,21 g L-1 ve biyo-bütanol verimi 0,18 ± 0,011 g/g şeker olarak bulundu. Bir sonraki çalışmada, geniş yelpazede bütanol üretimini inceleyebiliriz.

Keywords

• Biyo-bütanol üretimi
• Mikroalgler
• Atıksu
• Antioksidan enzimler

Biomass and Bio-butanol Production from Borodinellopsis texensis CCALA 892 in Synthetic Wastewater: Determination of Biochemical Composition

Abstract

Microalgae can generally maintain the high amounts of biomass in the wastewater and they can be converted from biomass to bio-butanol. Bio-butanol is a liquid biofuel and it has significant physical and chemical properties. In this study, we carried out bio-butanol production from Borodinellopsis texensis CCALA 892 grown in various concentrations of the municipal wastewater. Also, we determined biochemical composition ratios of microalgae samples and studied the some antioxidant enzymes such as catalase, superoxide dismutase and ascorbate peroxidase. In the current study, bio-butanol was produced by the acetone-butanol-ethanol (ABE) fermentation method. The microalgae sample grown in 25% of wastewater had the highest biomass productivity among five wastewater samples with 0.114 ± 0.002 g L-1d-1. The carbohydrate and protein concentrations of control group increased day by day and their values reached stationary phases at seven days. The sample grown in 25% of wastewater had the highest carbohydrate concentration with 0.30 g L-1 and protein concentration with 0.35 g L-1 at the stationary phase. The maximum enzyme activities for catalase, superoxide dismutase and ascorbate peroxidase were 15.33 ± 0.88, 8.67 ± 0.67 and 33 ± 1.53 µmole/mg, respectively at 25% of wastewater. In addition, bio-butanol content of B. texensis CCALA 892 was 3.63 ± 0.21 g L-1 and its bio-butanol yield was found as 0.18 ± 0.011 g/g sugar. In the next study, we can examine large scale butanol production.

Keywords

• Bio-butanol production
• Microalgae
• Wastewater
• Antioxidant enzymes

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