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Determination the Fatty Acid Composition of the Rumex patientia L. Leaves and in vitro Antimicrobial Activity of their Different Extracts

Year 2020, , 362 - 367, 26.08.2020
https://doi.org/10.19113/sdufenbed.643154

Abstract

Rumex patientia L. is from Polygonaceae family and there are 25 species of it in Turkey. Rumex patientia L. has antifebrile, laxative, diuretic, and pain killer properties. In this study, the extraction of the Rumex patientia L. leaf were done and determination the fat content and fatty acid composition by using GC device and to determinate in vitro antimicrobial activity of their different extracts were aimed. On the purpose of detection of antimicrobial activity; Rumex patientia L. leaf was extracted with ethanol, methanol, acetone, petroleum ether and water. Rumex patientia L. leaves was tested their different extracts using Bacillus subtilis, Bacillus cereus, Escherichia coli, Pseudomonas aeroginosa, Pasteurella multocida, Yersinia enterocolitica, Klebsiella pneumoniae, Staphylococcus aureus bacteria and Candida albicans yeast by agar-well diffusion technique. As a result of the study, 26 fatty acid components were detected on Rumex patientia L. leaf. In addition, among obtained extracts the highest antimicrobial activity was formed by methyl alcohol extract on K. pneumoniae bacteria (32mm).

References

  • [1] Davis, P. H. 1967. Flora of Turkey and East Aegean Islands. University press, Edinburgh, 2, 281–293.
  • [2] Davis, P. H. 1988. Flora of Turkey and East Aegean Islands. University press, Edinburgh, 10, 86–87.
  • [3] Baytop, T. 1996. Türkiye’de Bitkiler ile Tedavi. I.U. Yayinlari No. 3255, Eczacilik Fak. No. 40, Istanbul Universitesi Basimevi, Istanbul, 444s.
  • [4] Demirezer, L. Ö., Kuruüzüm-Uz, A., Bergere, I., Schiewe, H. J., Zeeck, A. 2001. The structures of antioxidant and cytotoxic agents from natural source: Anthraquinones and tannins from roots of Rumex patientia. Phytochemistry 58, 1213–1217
  • [5] Yuan, Y., Chen, W. S., Zheng, S. Q., Yang, G. J., Zhang, W. D., Zhang, H. M. 2001. Studies on chemical constituents in root of Rumex patientia L. Zhongguo Zhong yao za zhi= Zhongguo zhongyao zazhi= China journal of Chinese materia medica, 26(4), 256-258.
  • [6] Song, E. K., Hur, H., Han, M. K. 2003. Epigallocatechin gallate prevents autoimmune diabetes induced by multiple low doses of streptozotocin in mice. Archives of pharmacal research, 26(7), 559-563.
  • [7] Roghani, M., Baluchnejadmojarad, T. 2010. Hypoglycemic and hypolipidemic effect and antioxidant activity of chronic epigallocatechin-gallate in streptozotocin-diabetic rats. Pathophysiology, 17(1), 55-59.
  • [8] Anton, S., Melville, L., Rena, G. 2007. Epigallocatechin gallate (EGCG) mimics insulin action on the transcription factor FOXO1a and elicits cellular responses in the presence and absence of insulin. Cellular signalling, 19(2), 378-383.
  • [9] Lone, I. A., Kaur, G., Athar, M., Alam, M. S. 2007. Protective effect of Rumex patientia (English Spinach) roots on ferric nitrilotriacetate (Fe-NTA) induced hepatic oxidative stress and tumor promotion response. Food and chemical toxicology, 45(10), 1821-1829.
  • [10] Sedaghat, R., Roghani, M., Ahmadi, M., Ahmadi, F. 2011. Antihyperglycemic and antihyperlipidemic effect of Rumex patientia seed preparation in streptozotocin-diabetic rats. Pathophysiology, 18(2), 111-115.
  • [11] Liu, J., Xia, Z. T., Zhou, G. R., Zhang, L. L., Kong, L. Y. 2011. Study on the chemical constituents of Rumex Patientia. Zhong yao cai= Zhongyaocai= Journal of Chinese medicinal materials, 34(6), 893-895.
  • [12] Vasas, A., Orbán-Gyapai, O., Hohmann, J. 2015. The genus rumex: review of traditional uses, phytochemistry and pharmacology, Journal of Ethnopharmacology, 175, 198-228.
  • [13] Jeppesen, A. S,, Soelberg, J., Jäger, A. K. 2012. Antibacterial and COX-1 inhibitory effect of medicinal plants from the Pamir Mountains. Afghanistan, Plants, 1(2), 74-81.
  • [14] Nas, S., Gökalp, H. Y., Ünsal, M. 2001. Bitkisel Yağ Teknolojisi. Pamukkale Üniversitesi Mühendislik Fakültesi Ders Kitapları Yayın No: 5, Denizli, 329s.
  • [15] Submuth, R., Eberspaecher, J., Haag, R., Sprınger, W. 1987. Biochemisch Mikrobioogisches Prakticum, Thieme Verlag, Stuttgart, 409s.
  • [16] Getie, M., Gebre-Mariam, T., Rietz, R., Höhne, C., Huschka, C., Schmidtke, M., Neubert, R. H. H. 2003. Evaluation of the anti-microbial and anti-inflammatory activities of the medicinal plants Dodonaea viscosa, Rumex nervosus and Rumex abyssinicus. Fitoterapia, 74(1-2), 139-143.
  • [17] Mostafa, H. A. M., El-Bakry, A. A., Alam, E. A. 2012. Evaluation of antibacterial activity of different plant parts of Rumex vesicarius L. at early and late vegetative stages of growth. International Journal of Pharmacy and Pharmaceutical Sciences, 4, 426-435.
  • [18] Kumar, S. U. R. J. E. E. T., Lincy Joseph, L., George, M. A. T. H. E. W., Bharti, V. I. V. E. K. 2011. Antimicrobial activity of methanolic extracts of Rumex nepalensis leaves. International Journal of Pharmacy and Pharmaceutical Sciences, 3(4), 240-242.
  • [19] Mungole, A., Chaturvedi, A. 2011. Determination of antibacterial activity of two medicinally important Indian Taxa. Der Pharma Chemica, 3, 83-89.
  • [20] Yadav, S., Kumar, S., Jain, P., Pundir, R. K., Jadon, S., Sharma, A., Khetwal, K. S., Gupta, K. C. 2011. Antimicrobial activity of different extracts of roots of Rumex nepalensis Spreng. Indian Journal of Natural Products anda Resources, 2(1), 65-69.
  • [21] Yildirim, A., Mavi, A., Kara, A. A. 2001. Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts. Journal of Agricultural Food Chemistry, 49(8) 4083–4089.
  • [22] Orbán-Gyapai, O., Liktor-Busa, E., Kúsz, N., Stefkó, D., Urbán, E., Hohmann, J., Vasas, A. 2017. Antibacterial screening of Rumex species native to the Carpathian Basin and bioactivity-guided isolation of compounds from Rumex aquaticus. Fitoterapia, 118, 101-106.
  • [23] Omarova, M. A., Artamonova, N. A., Chasovitina, G. M. 1998. Chemical composition of the hybrid Rumex K-1. Chemistry of natural compounds, 34(4), 426-428.
  • [24] Gogus, U., Smith, C. 2010. n-3 Omega fatty acids: a review of current knowledge. International Journal of Food Science and Technology, 45, 417–436.
  • [25] Champe, P. C., Harvey, R. A. 1997. Lippincott’s illustrated reviews serisinden: Biyokimya. Çeviri. Uludağ Üniversitesi Tıp Fakültesi Biyokimya Anabilim Dalı. Nobel Tıp Kitapevleri, İstanbul, 173 s.

Rumex patientia L. Yapraklarının Yağ Asitleri Kompozisyonunun ve Farklı Ekstraktlarının in vitro Antimikrobiyal Aktivitesinin Belirlenmesi

Year 2020, , 362 - 367, 26.08.2020
https://doi.org/10.19113/sdufenbed.643154

Abstract

Rumex patientia L. (efelek) Polygonaceae familyasından olup, Türkiye’de 25 türü bulunmaktadır. Rumex patientia L. ateş düşürücü, kabızlığı giderici, idrar söktürücü, yara iyileştirici ve ağrı kesici özelliklere sahiptir. Yapılan çalışmada, Rumex patientia L. yaprağının ekstraksiyonu yapılarak yağ oranı ve yağ asidi kompozisyonu GC cihazı kullanılarak belirlenmesi ve farklı ekstraklarının in vitro antimikrobiyal aktivitesinin tespit edilmesi amaçlanmıştır. Antimikrobiyal aktivite tayini için; Rumex patientia L. (efelek) yaprağının etanol, metanol, aseton, petrol eteri ve su ile ekstraktı çıkarılmıştır. Bacillus subtilis, Bacillus cereus, Escherichia coli, Pseudomonas aeroginosa, Pasteurella multocida, Yersinia enterocolitica, Klebsiella pneumoniae, Staphylococcus aureus bakterileri ve Candida albicans mayası kullanılarak agar kuyucuk difüzyon tekniği ile Rumex patientia L. (efelek) yaprağının farklı ekstraktları test edilmiştir. Çalışma sonucunda, Rumex patientia L. yaprağında toplam 26 adet yağ asit bileşenleri tespit edilmiştir. Ayrıca elde edilen ekstraktlardan antimikrobiyal olarak en yüksek aktivitenin; K. pneumoniae (32mm) bakterisi üzerine metil alkol ekstraktı tarafından oluşturulduğu görülmüştür.

References

  • [1] Davis, P. H. 1967. Flora of Turkey and East Aegean Islands. University press, Edinburgh, 2, 281–293.
  • [2] Davis, P. H. 1988. Flora of Turkey and East Aegean Islands. University press, Edinburgh, 10, 86–87.
  • [3] Baytop, T. 1996. Türkiye’de Bitkiler ile Tedavi. I.U. Yayinlari No. 3255, Eczacilik Fak. No. 40, Istanbul Universitesi Basimevi, Istanbul, 444s.
  • [4] Demirezer, L. Ö., Kuruüzüm-Uz, A., Bergere, I., Schiewe, H. J., Zeeck, A. 2001. The structures of antioxidant and cytotoxic agents from natural source: Anthraquinones and tannins from roots of Rumex patientia. Phytochemistry 58, 1213–1217
  • [5] Yuan, Y., Chen, W. S., Zheng, S. Q., Yang, G. J., Zhang, W. D., Zhang, H. M. 2001. Studies on chemical constituents in root of Rumex patientia L. Zhongguo Zhong yao za zhi= Zhongguo zhongyao zazhi= China journal of Chinese materia medica, 26(4), 256-258.
  • [6] Song, E. K., Hur, H., Han, M. K. 2003. Epigallocatechin gallate prevents autoimmune diabetes induced by multiple low doses of streptozotocin in mice. Archives of pharmacal research, 26(7), 559-563.
  • [7] Roghani, M., Baluchnejadmojarad, T. 2010. Hypoglycemic and hypolipidemic effect and antioxidant activity of chronic epigallocatechin-gallate in streptozotocin-diabetic rats. Pathophysiology, 17(1), 55-59.
  • [8] Anton, S., Melville, L., Rena, G. 2007. Epigallocatechin gallate (EGCG) mimics insulin action on the transcription factor FOXO1a and elicits cellular responses in the presence and absence of insulin. Cellular signalling, 19(2), 378-383.
  • [9] Lone, I. A., Kaur, G., Athar, M., Alam, M. S. 2007. Protective effect of Rumex patientia (English Spinach) roots on ferric nitrilotriacetate (Fe-NTA) induced hepatic oxidative stress and tumor promotion response. Food and chemical toxicology, 45(10), 1821-1829.
  • [10] Sedaghat, R., Roghani, M., Ahmadi, M., Ahmadi, F. 2011. Antihyperglycemic and antihyperlipidemic effect of Rumex patientia seed preparation in streptozotocin-diabetic rats. Pathophysiology, 18(2), 111-115.
  • [11] Liu, J., Xia, Z. T., Zhou, G. R., Zhang, L. L., Kong, L. Y. 2011. Study on the chemical constituents of Rumex Patientia. Zhong yao cai= Zhongyaocai= Journal of Chinese medicinal materials, 34(6), 893-895.
  • [12] Vasas, A., Orbán-Gyapai, O., Hohmann, J. 2015. The genus rumex: review of traditional uses, phytochemistry and pharmacology, Journal of Ethnopharmacology, 175, 198-228.
  • [13] Jeppesen, A. S,, Soelberg, J., Jäger, A. K. 2012. Antibacterial and COX-1 inhibitory effect of medicinal plants from the Pamir Mountains. Afghanistan, Plants, 1(2), 74-81.
  • [14] Nas, S., Gökalp, H. Y., Ünsal, M. 2001. Bitkisel Yağ Teknolojisi. Pamukkale Üniversitesi Mühendislik Fakültesi Ders Kitapları Yayın No: 5, Denizli, 329s.
  • [15] Submuth, R., Eberspaecher, J., Haag, R., Sprınger, W. 1987. Biochemisch Mikrobioogisches Prakticum, Thieme Verlag, Stuttgart, 409s.
  • [16] Getie, M., Gebre-Mariam, T., Rietz, R., Höhne, C., Huschka, C., Schmidtke, M., Neubert, R. H. H. 2003. Evaluation of the anti-microbial and anti-inflammatory activities of the medicinal plants Dodonaea viscosa, Rumex nervosus and Rumex abyssinicus. Fitoterapia, 74(1-2), 139-143.
  • [17] Mostafa, H. A. M., El-Bakry, A. A., Alam, E. A. 2012. Evaluation of antibacterial activity of different plant parts of Rumex vesicarius L. at early and late vegetative stages of growth. International Journal of Pharmacy and Pharmaceutical Sciences, 4, 426-435.
  • [18] Kumar, S. U. R. J. E. E. T., Lincy Joseph, L., George, M. A. T. H. E. W., Bharti, V. I. V. E. K. 2011. Antimicrobial activity of methanolic extracts of Rumex nepalensis leaves. International Journal of Pharmacy and Pharmaceutical Sciences, 3(4), 240-242.
  • [19] Mungole, A., Chaturvedi, A. 2011. Determination of antibacterial activity of two medicinally important Indian Taxa. Der Pharma Chemica, 3, 83-89.
  • [20] Yadav, S., Kumar, S., Jain, P., Pundir, R. K., Jadon, S., Sharma, A., Khetwal, K. S., Gupta, K. C. 2011. Antimicrobial activity of different extracts of roots of Rumex nepalensis Spreng. Indian Journal of Natural Products anda Resources, 2(1), 65-69.
  • [21] Yildirim, A., Mavi, A., Kara, A. A. 2001. Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts. Journal of Agricultural Food Chemistry, 49(8) 4083–4089.
  • [22] Orbán-Gyapai, O., Liktor-Busa, E., Kúsz, N., Stefkó, D., Urbán, E., Hohmann, J., Vasas, A. 2017. Antibacterial screening of Rumex species native to the Carpathian Basin and bioactivity-guided isolation of compounds from Rumex aquaticus. Fitoterapia, 118, 101-106.
  • [23] Omarova, M. A., Artamonova, N. A., Chasovitina, G. M. 1998. Chemical composition of the hybrid Rumex K-1. Chemistry of natural compounds, 34(4), 426-428.
  • [24] Gogus, U., Smith, C. 2010. n-3 Omega fatty acids: a review of current knowledge. International Journal of Food Science and Technology, 45, 417–436.
  • [25] Champe, P. C., Harvey, R. A. 1997. Lippincott’s illustrated reviews serisinden: Biyokimya. Çeviri. Uludağ Üniversitesi Tıp Fakültesi Biyokimya Anabilim Dalı. Nobel Tıp Kitapevleri, İstanbul, 173 s.
There are 25 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Articles
Authors

Elife Kaya 0000-0001-7213-3601

Perihan Akbaş 0000-0001-5977-7621

Gökhan Ceyhan This is me 0000-0002-9127-2348

Tuğba Karabekmez Erdem 0000-0002-9403-7108

Hiçran Alkan This is me 0000-0002-8720-465X

Publication Date August 26, 2020
Published in Issue Year 2020

Cite

APA Kaya, E., Akbaş, P., Ceyhan, G., Karabekmez Erdem, T., et al. (2020). Determination the Fatty Acid Composition of the Rumex patientia L. Leaves and in vitro Antimicrobial Activity of their Different Extracts. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 24(2), 362-367. https://doi.org/10.19113/sdufenbed.643154
AMA Kaya E, Akbaş P, Ceyhan G, Karabekmez Erdem T, Alkan H. Determination the Fatty Acid Composition of the Rumex patientia L. Leaves and in vitro Antimicrobial Activity of their Different Extracts. Süleyman Demirel Üniv. Fen Bilim. Enst. Derg. August 2020;24(2):362-367. doi:10.19113/sdufenbed.643154
Chicago Kaya, Elife, Perihan Akbaş, Gökhan Ceyhan, Tuğba Karabekmez Erdem, and Hiçran Alkan. “Determination the Fatty Acid Composition of the Rumex Patientia L. Leaves and in Vitro Antimicrobial Activity of Their Different Extracts”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 24, no. 2 (August 2020): 362-67. https://doi.org/10.19113/sdufenbed.643154.
EndNote Kaya E, Akbaş P, Ceyhan G, Karabekmez Erdem T, Alkan H (August 1, 2020) Determination the Fatty Acid Composition of the Rumex patientia L. Leaves and in vitro Antimicrobial Activity of their Different Extracts. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 24 2 362–367.
IEEE E. Kaya, P. Akbaş, G. Ceyhan, T. Karabekmez Erdem, and H. Alkan, “Determination the Fatty Acid Composition of the Rumex patientia L. Leaves and in vitro Antimicrobial Activity of their Different Extracts”, Süleyman Demirel Üniv. Fen Bilim. Enst. Derg., vol. 24, no. 2, pp. 362–367, 2020, doi: 10.19113/sdufenbed.643154.
ISNAD Kaya, Elife et al. “Determination the Fatty Acid Composition of the Rumex Patientia L. Leaves and in Vitro Antimicrobial Activity of Their Different Extracts”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 24/2 (August 2020), 362-367. https://doi.org/10.19113/sdufenbed.643154.
JAMA Kaya E, Akbaş P, Ceyhan G, Karabekmez Erdem T, Alkan H. Determination the Fatty Acid Composition of the Rumex patientia L. Leaves and in vitro Antimicrobial Activity of their Different Extracts. Süleyman Demirel Üniv. Fen Bilim. Enst. Derg. 2020;24:362–367.
MLA Kaya, Elife et al. “Determination the Fatty Acid Composition of the Rumex Patientia L. Leaves and in Vitro Antimicrobial Activity of Their Different Extracts”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol. 24, no. 2, 2020, pp. 362-7, doi:10.19113/sdufenbed.643154.
Vancouver Kaya E, Akbaş P, Ceyhan G, Karabekmez Erdem T, Alkan H. Determination the Fatty Acid Composition of the Rumex patientia L. Leaves and in vitro Antimicrobial Activity of their Different Extracts. Süleyman Demirel Üniv. Fen Bilim. Enst. Derg. 2020;24(2):362-7.

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