Determination the Fatty Acid Composition of the Rumex patientia L. Leaves and in vitro Antimicrobial Activity of their Different Extracts

Öz

Rumex patientia L. is from Polygonaceae family and there are 25 species of it in Turkey. Rumex patientia L. has antifebrile, laxative, diuretic, and pain killer properties. In this study, the extraction of the Rumex patientia L. leaf were done and determination the fat content and fatty acid composition by using GC device and to determinate in vitro antimicrobial activity of their different extracts were aimed. On the purpose of detection of antimicrobial activity; Rumex patientia L. leaf was extracted with ethanol, methanol, acetone, petroleum ether and water. Rumex patientia L. leaves was tested their different extracts using Bacillus subtilis, Bacillus cereus, Escherichia coli, Pseudomonas aeroginosa, Pasteurella multocida, Yersinia enterocolitica, Klebsiella pneumoniae, Staphylococcus aureus bacteria and Candida albicans yeast by agar-well diffusion technique. As a result of the study, 26 fatty acid components were detected on Rumex patientia L. leaf. In addition, among obtained extracts the highest antimicrobial activity was formed by methyl alcohol extract on K. pneumoniae bacteria (32mm).

Anahtar Kelimeler

• Antimicrobial
• Fatty acid composition
• Rumex patientia L.

Rumex patientia L. Yapraklarının Yağ Asitleri Kompozisyonunun ve Farklı Ekstraktlarının in vitro Antimikrobiyal Aktivitesinin Belirlenmesi

Öz

Rumex patientia L. (efelek) Polygonaceae familyasından olup, Türkiye’de 25 türü bulunmaktadır. Rumex patientia L. ateş düşürücü, kabızlığı giderici, idrar söktürücü, yara iyileştirici ve ağrı kesici özelliklere sahiptir. Yapılan çalışmada, Rumex patientia L. yaprağının ekstraksiyonu yapılarak yağ oranı ve yağ asidi kompozisyonu GC cihazı kullanılarak belirlenmesi ve farklı ekstraklarının in vitro antimikrobiyal aktivitesinin tespit edilmesi amaçlanmıştır. Antimikrobiyal aktivite tayini için; Rumex patientia L. (efelek) yaprağının etanol, metanol, aseton, petrol eteri ve su ile ekstraktı çıkarılmıştır. Bacillus subtilis, Bacillus cereus, Escherichia coli, Pseudomonas aeroginosa, Pasteurella multocida, Yersinia enterocolitica, Klebsiella pneumoniae, Staphylococcus aureus bakterileri ve Candida albicans mayası kullanılarak agar kuyucuk difüzyon tekniği ile Rumex patientia L. (efelek) yaprağının farklı ekstraktları test edilmiştir. Çalışma sonucunda, Rumex patientia L. yaprağında toplam 26 adet yağ asit bileşenleri tespit edilmiştir. Ayrıca elde edilen ekstraktlardan antimikrobiyal olarak en yüksek aktivitenin; K. pneumoniae (32mm) bakterisi üzerine metil alkol ekstraktı tarafından oluşturulduğu görülmüştür.

Anahtar Kelimeler

• Antimikrobiyal
• Yağ asit kompozisyonu
• Rumex patientia L.

Kaynakça

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