A Preliminary Research on Microspore Culture in Eggplant (Solanum melongena L.)

Year 2019, Volume: 23 Issue: Special [en], 61 - 66, 01.03.2019

Buse Çelik , Ahmet Naci Onus

https://doi.org/10.19113/sdufenbed.431497

Cited By: 1

Abstract

 Microspore
embryogenesis is a process in which immature male gametophytes are induced to
divert them from their gametophytic pathway toward embryo development during in vitro culture. In this study,
therefore, it was aimed to determine the response of two eggplant cultivars to
microspore culture. For this purpose, the microspores were firstly isolated
from the anthers at the appropriate stage of microspore development (containing
mostly vacuolate microspores and young bicellular pollen) and subjected to pre-treatment
at 35°C  for 3 days in dark conditions.
After pre-treatment, the microspores were cultured in liquid NLN culture medium
supplemented with 2% sucrose, 0.5 mg/l naphthaleneacetic acid (NAA), and 0.5
mg/l, 6-benzylaminopurine (BAP), pH 5.9, and kept in the dark at 25°C for one
month. During this culture process, the microspore embryogenesis induction was
microscopically analyzed and focused on the initial stages of this
developmental process. Immadiately after induction, before the microspores develop
into callus, they were induced to divide symmetrically and form multinucleated
structures, and then microspores did not form direct embryos and formed callus.
At the end of one month, only callus formation occurred from microspores and
total callus number per petri was 
analyzed. The average calli for G07-1 cultivars was 288 calli/petri,
while it was 64 calli/petri dish for G07-2 cultivars. It is thought that this
research will guide both practical and basic researches on the development of
microspore culture technique.

Keywords

Eggplant, Microspore culture, Haploid, DH, Microspore embryogenesis

Patlıcanda (Solanum melongena L.) Mikrospor Kültürü Üzerine Bir Ön Araştırma

Abstract

Mikrospor
embriyogenesis olgunlaşmamış erkek gametofitlerin in vitro kültür süresince gametofitik gelişimden embriyo oluşturmak
üzere uyarıldığı bir sistemdir. Bu araştırmada, iki adet patlıcan (Solanum melongena L.) çeşidinin
mikrospor kültür tekniğine tepkisinin belirlenmesi amaçlanmıştır. Bu amaçla,
ilk olarak uygun mikrospor gelişme dönemindeki mikrosporlar (çoğunluğu vakuol
mikrospor ve genç çift çekirdekli polen) anterlerden izole edilerek 35°C‘de 3
gün karanlık koşullarda ön uygulamaya maruz bırakılmıştır. Ön uygulama
işleminden sonra mikrosporlar %2 sakkaroz, 0.5 mg/l naphthaleneacetic acid
(NAA) ve 0.5 mg/l 6-benzylaminopurine (BAP), pH 5.9, içeren NLN ortamında
kültüre alınmış ve bir ay boyunca 25°C‘de karanlıkta bekletilmiştir. Kültür
süreci boyunca mikrospor embriyogenesis indüksiyon süreci mikroskobik olarak
analiz edilerek bu gelişimsel sapmanın ilk evrelerine odaklanılmıştır.
Mikrosporların indüksiyondan hemen sonra kallus haline gelmeden önce simetrik
bölünme ve çok çekirdekli yapılar meydana getirdiği daha sonra ise
mikrosporların direkt embriyo oluşturmadıkları ve kallus oluşturduğu tespit
edilmiştir. Araştırmada bir ay sonunda mikrosporlardan yalnızca kallus oluşumu
meydana gelmiştir ve petri başına toplam kallus sayısı belirlenmiştir. G07-1 çeşidinde
ortalama 288 kallus/petri elde edilirken G07-2 çeşidinde 64 kallus/petri
meydana gelmiştir. Bu araştırmanın mikrospor kültürü tekniğinin
geliştirilebilmesi üzerine hem uygulamalı, hem de temel araştırmalar için yol
gösterici olacağı düşünülmektedir.

Keywords

Patlıcan, Mikrospor kültürü, Haploid, DH, mikrospor embriyogenesis

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