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A Preliminary Research on Microspore Culture in Eggplant (Solanum melongena L.)

Yıl 2019, Cilt: 23 Sayı: Özel, 61 - 66, 01.03.2019
https://doi.org/10.19113/sdufenbed.431497

Öz

 Microspore
embryogenesis is a process in which immature male gametophytes are induced to
divert them from their gametophytic pathway toward embryo development during in vitro culture. In this study,
therefore, it was aimed to determine the response of two eggplant cultivars to
microspore culture. For this purpose, the microspores were firstly isolated
from the anthers at the appropriate stage of microspore development (containing
mostly vacuolate microspores and young bicellular pollen) and subjected to pre-treatment
at 35°C  for 3 days in dark conditions.
After pre-treatment, the microspores were cultured in liquid NLN culture medium
supplemented with 2% sucrose, 0.5 mg/l naphthaleneacetic acid (NAA), and 0.5
mg/l, 6-benzylaminopurine (BAP), pH 5.9, and kept in the dark at 25°C for one
month. During this culture process, the microspore embryogenesis induction was
microscopically analyzed and focused on the initial stages of this
developmental process. Immadiately after induction, before the microspores develop
into callus, they were induced to divide symmetrically and form multinucleated
structures, and then microspores did not form direct embryos and formed callus.
At the end of one month, only callus formation occurred from microspores and
total callus number per petri was 
analyzed. The average calli for G07-1 cultivars was 288 calli/petri,
while it was 64 calli/petri dish for G07-2 cultivars. It is thought that this
research will guide both practical and basic researches on the development of
microspore culture technique.

Kaynakça

  • [1] Forster, B.P., Herberle-Bors, E., Kasha, K.J., Touraev, A. 2007. The resurgence of haploids in higher plants. Trends in Plant Science, 12(8), 368–375.
  • [2] Dunwell, J.M. 2010. Haploids in flowering plants: origins and exploitation. Plant Biotechnol J., 8: 377-424.
  • [3] Germanà, M. 2011. Gametic embryogenesis and haploid technology as valuable support to plant breeding. Plant Cell Rep., 30: 839-857.
  • [4] Segui-Simarro, J.M. 2010. Androgenesis revisited. The Botanical Review., 76: 377-404.
  • [5] Maluszynski, M., Kasha, K.J., Szarejko, I. 2003. Published doubled haploid protocols in plant species. pp 309-335. Maluszynski, M., Kasha, K.J., Forster, B.P., Szarejko, I. eds. 2003. Doubled haploid production in crop plants A manual. Kluwer Academic Publishers, Dordrecht, Netherlands, 428.
  • [6] Maraschin, S.F., De-Priester, W., Spaink, H.P., Wang, M. 2005a. Androgenic switch: an example of plant embryogenesis from the male gametophyte perspective. J Exp Bot., 56: 1711–1726.
  • [7] Segui-Simarro, J.M., Corral-Martinez, P., Parra-Vega, V., Gonzalez-Garcia, B. 2011. Androgenesis in recalcitrant solanaceous crops. Plant Cell Rep., 30: 765-778.
  • [8] Raina, S.K., Iyer, R.D. 1973. Differentiation of diploid plants from pollen callus in anther cultures of Solanum melongena L. Z Pflanzenzucht, 70: 275-280.
  • [9] Isouard, G., Raquin, C., Demarly, Y. 1979. Obtention de plantes haploides et diploides par culture in vitro d'anthères dáubergine (Solanum melongena L.). C R Acad Sci, Paris, 288: 987-989.
  • [10] Dumas de Vaulx, R., Chambonnet, D. 1982. Culture in vitro d'anthères d'aubergine (Solanum melongena L.): stimulation de la production de plantes au moyen de traitements à 35ºC associés à de faibles teneurs en substances de croissance. Agronomie, 2: 983-988.
  • [11] Misra, N.R., Varghese, T.M., Maherchandani, N., Jain, R.K. 1983. Studies on induction and differentiation of androgenic callus of Solanum melongena L. pp 465-468. Sen, S.K., Giles, K.L. eds. 1983. Plant cell culture in crop improvement. Plenum Press, New York, 502p.
  • [12] Borgel, A., Arnaud, M. 1986. Progress in eggplant breeding, use of haplomethod. Capsicum Newsl, 5:65–66.
  • [13] Rotino, G.L., Falavigna, A., Restaino, F. 1987. Production of anther-derived plantlets of eggplant. Capsicum Newsl, 6:89–90.
  • [14] Tuberosa, R., Sanghineti, M.C., Conti, S. 1987. Anther culture of eggplant Solanum melongena L. lines and hybrids. Gene´tica Agra´ria, 41:267–274.
  • [15] Rotino, G.L. 1996. Haploidy in eggplant. pp 115-141. Jain, S.M., Sopory, S.K., Veilleux, R.E. eds. 1996. In vitro haploid production in higher plants, Kluwer Academic Publishers, Dordrecht, The Netherlands, 412p.
  • [16] Rotino, G.L., Sihachakr, D., Rizza, F., Vale, G., Tacconi, M.G., Alberti, P., Mennella, G., Sabatini, E., Toppino, L., D'Alessandro, A., Acciarri, N. 2005. Current status in production and utilization of dihaploids from somatic hybrids between eggplant (Solanum melongena L.) and its wild relatives. Acta Physiol Plant, 27: 723-733.
  • [17] Alpsoy, H.C., Seniz, V. 2007. Researches on the in vitro androgenesis and obtaining haploid plants in some eggplant genotypes. Acta Hortic, 729:137–141.
  • [18] Salas, P., Prohens, J., Seguı´-Simarro, J.M. 2011. Evaluation of androgenic competence through anther culture in common eggplant and related species. Euphytica, 182:261–274.
  • [19] Salas, P., Rivas-Sendra, A., Prohens, J., Segui-Simarro, J.M. 2012. Influence of the stage for anther excision and heterostyly in embryogenesis induction from eggplant anther cultures. Euphytica, 184:235–250.
  • [20] Başay, S., Ellialtıoğlu, Ş. 2012. Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.). Turk J Biol, 37: 499-505.
  • [21] Gu, S.R. 1979. Plantlets from isolated pollen cultures of eggplant (Solanum melongena L.). Acta Bot Sin, 21: 30–36.
  • [22] Miyoshi, K. 1996. Callus induction and plantlet formation through culture of isolated microspores of eggplant (Solanum melongena L.). Plant Cell Rep, 15:391–395.
  • [23] Corral-Martinez, P., Segui-Simarro, J.M. 2012. Efficient production of callus-derived doubled haploids through isolated microspore culture in eggplant (Solanum melongena L.). Euphytica, 187:47–61.
  • [24] Corral-Martinez, P., Segui-Simarro, J.M. 2014. Refining the method for eggplant microspore culture: effect of abscisic acid, epibrassinolide, polyethylene glycol, naphthaleneacetic acid, 6-benzylaminopurine and arabinogalactan proteins. Euphytica, 195: 369–382.
  • [25] Bal, U., Abak, K. 2005. Induction of Symmetrical Nucleus Division and Multicellular Structures from the Isolated Microspores of Lycopersicon Esculentum Mill. Biotechnology & Biotechnological Equipment, 19(1): 35-42.
  • [26] Bal, U., Ellialtioglu, S., Abak, K. 2009. Induction of symmetrical nucleus division and multi-nucleate structures in microspores of eggplant (Solanum melongena L.) cultured in vitro. Sci Agric. 66: 535–539.
  • [27] Tuncer, B., Yanmaz, R. 2011a. Effects of colchicine and high temperature treatments on isolated microspore culture in various cabbage (Brassica oleraceae) types. International Journal of Agriculture and Biology, 13(5): 819-822.
  • [28] Tuncer, B., Yanmaz, R. 2011b. Induction of microspore embryogenesis in ornamental kale by gamma irradiation and high temperature stress. Asian Journal of Biotechnology, 3: 415-421.
  • [29] Tuncer, B., Cig, A., Yanmaz, R., Yasar, F. 2016. Effect of heat shock treatment on microspore embryogenesis in Brassica oleracea species. Journal of Agricultural Scieces, 22(4): 548-554.
  • [30] Tuncer, B. 2017. Callus formation from isolated microspore culture in radish (Raphanus sativus L.). The Journal of Animal & Plant Sciences, 27(1): 277-282.
  • [31] Custers, J.B.M. 2003. Microspore culture in rapeseed (Brassica napus L.). pp 185-194. Maluszynski, M., Kasha, K.J., Forster, B.P., Szarejko, I. Eds. 2003, Doubled haploid production in crop plants: a manual. Kluwer Academic Publishers, Dordrecht, Netherlands, 428.
  • [32] Touraev, A., Pfosser, M., Heberle-Bors, E. 2001. The microspore: a haploid multipurpose cell. Adv Bot Res, 35:53–109.
  • [33] Segui-Simarro, J.M., Nuez, F. 2008. How microspores transform into haploid embryos: changes associated with embryogenesis induction and microspore-derived embryogenesis. Physiologia Plantarum, 134: 1-12.
  • [34] Indrianto, A., Barinova, I., Touraev, A., Heberle-Bors, E. 2001. Tracking individual wheat microspores in vitro: identification of embryogenic microspores and body axis formation in the embryo. Planta, 212: 163–174.
  • [35] Maraschin, S.F., Vennik, M., Lamers, G.E.M., Spaink, H.P., Wang, M. 2005. Time-lapse tracking of barley androgenesis reveals position-determined cell death within pro-embryos. Planta, 220: 531–540.
  • [36] Zaki, M.A.M., Dickinson, H.G. 1991. Microspore-derived embryos in Brassica: the significance of division symmetry in pollen mitosis I to embryogenic development. Sexual Plant Reproduction, 4:48–55.

Patlıcanda (Solanum melongena L.) Mikrospor Kültürü Üzerine Bir Ön Araştırma

Yıl 2019, Cilt: 23 Sayı: Özel, 61 - 66, 01.03.2019
https://doi.org/10.19113/sdufenbed.431497

Öz

Mikrospor
embriyogenesis olgunlaşmamış erkek gametofitlerin in vitro kültür süresince gametofitik gelişimden embriyo oluşturmak
üzere uyarıldığı bir sistemdir. Bu araştırmada, iki adet patlıcan (Solanum melongena L.) çeşidinin
mikrospor kültür tekniğine tepkisinin belirlenmesi amaçlanmıştır. Bu amaçla,
ilk olarak uygun mikrospor gelişme dönemindeki mikrosporlar (çoğunluğu vakuol
mikrospor ve genç çift çekirdekli polen) anterlerden izole edilerek 35°C‘de 3
gün karanlık koşullarda ön uygulamaya maruz bırakılmıştır. Ön uygulama
işleminden sonra mikrosporlar %2 sakkaroz, 0.5 mg/l naphthaleneacetic acid
(NAA) ve 0.5 mg/l 6-benzylaminopurine (BAP), pH 5.9, içeren NLN ortamında
kültüre alınmış ve bir ay boyunca 25°C‘de karanlıkta bekletilmiştir. Kültür
süreci boyunca mikrospor embriyogenesis indüksiyon süreci mikroskobik olarak
analiz edilerek bu gelişimsel sapmanın ilk evrelerine odaklanılmıştır.
Mikrosporların indüksiyondan hemen sonra kallus haline gelmeden önce simetrik
bölünme ve çok çekirdekli yapılar meydana getirdiği daha sonra ise
mikrosporların direkt embriyo oluşturmadıkları ve kallus oluşturduğu tespit
edilmiştir. Araştırmada bir ay sonunda mikrosporlardan yalnızca kallus oluşumu
meydana gelmiştir ve petri başına toplam kallus sayısı belirlenmiştir. G07-1 çeşidinde
ortalama 288 kallus/petri elde edilirken G07-2 çeşidinde 64 kallus/petri
meydana gelmiştir. Bu araştırmanın mikrospor kültürü tekniğinin
geliştirilebilmesi üzerine hem uygulamalı, hem de temel araştırmalar için yol
gösterici olacağı düşünülmektedir.

Kaynakça

  • [1] Forster, B.P., Herberle-Bors, E., Kasha, K.J., Touraev, A. 2007. The resurgence of haploids in higher plants. Trends in Plant Science, 12(8), 368–375.
  • [2] Dunwell, J.M. 2010. Haploids in flowering plants: origins and exploitation. Plant Biotechnol J., 8: 377-424.
  • [3] Germanà, M. 2011. Gametic embryogenesis and haploid technology as valuable support to plant breeding. Plant Cell Rep., 30: 839-857.
  • [4] Segui-Simarro, J.M. 2010. Androgenesis revisited. The Botanical Review., 76: 377-404.
  • [5] Maluszynski, M., Kasha, K.J., Szarejko, I. 2003. Published doubled haploid protocols in plant species. pp 309-335. Maluszynski, M., Kasha, K.J., Forster, B.P., Szarejko, I. eds. 2003. Doubled haploid production in crop plants A manual. Kluwer Academic Publishers, Dordrecht, Netherlands, 428.
  • [6] Maraschin, S.F., De-Priester, W., Spaink, H.P., Wang, M. 2005a. Androgenic switch: an example of plant embryogenesis from the male gametophyte perspective. J Exp Bot., 56: 1711–1726.
  • [7] Segui-Simarro, J.M., Corral-Martinez, P., Parra-Vega, V., Gonzalez-Garcia, B. 2011. Androgenesis in recalcitrant solanaceous crops. Plant Cell Rep., 30: 765-778.
  • [8] Raina, S.K., Iyer, R.D. 1973. Differentiation of diploid plants from pollen callus in anther cultures of Solanum melongena L. Z Pflanzenzucht, 70: 275-280.
  • [9] Isouard, G., Raquin, C., Demarly, Y. 1979. Obtention de plantes haploides et diploides par culture in vitro d'anthères dáubergine (Solanum melongena L.). C R Acad Sci, Paris, 288: 987-989.
  • [10] Dumas de Vaulx, R., Chambonnet, D. 1982. Culture in vitro d'anthères d'aubergine (Solanum melongena L.): stimulation de la production de plantes au moyen de traitements à 35ºC associés à de faibles teneurs en substances de croissance. Agronomie, 2: 983-988.
  • [11] Misra, N.R., Varghese, T.M., Maherchandani, N., Jain, R.K. 1983. Studies on induction and differentiation of androgenic callus of Solanum melongena L. pp 465-468. Sen, S.K., Giles, K.L. eds. 1983. Plant cell culture in crop improvement. Plenum Press, New York, 502p.
  • [12] Borgel, A., Arnaud, M. 1986. Progress in eggplant breeding, use of haplomethod. Capsicum Newsl, 5:65–66.
  • [13] Rotino, G.L., Falavigna, A., Restaino, F. 1987. Production of anther-derived plantlets of eggplant. Capsicum Newsl, 6:89–90.
  • [14] Tuberosa, R., Sanghineti, M.C., Conti, S. 1987. Anther culture of eggplant Solanum melongena L. lines and hybrids. Gene´tica Agra´ria, 41:267–274.
  • [15] Rotino, G.L. 1996. Haploidy in eggplant. pp 115-141. Jain, S.M., Sopory, S.K., Veilleux, R.E. eds. 1996. In vitro haploid production in higher plants, Kluwer Academic Publishers, Dordrecht, The Netherlands, 412p.
  • [16] Rotino, G.L., Sihachakr, D., Rizza, F., Vale, G., Tacconi, M.G., Alberti, P., Mennella, G., Sabatini, E., Toppino, L., D'Alessandro, A., Acciarri, N. 2005. Current status in production and utilization of dihaploids from somatic hybrids between eggplant (Solanum melongena L.) and its wild relatives. Acta Physiol Plant, 27: 723-733.
  • [17] Alpsoy, H.C., Seniz, V. 2007. Researches on the in vitro androgenesis and obtaining haploid plants in some eggplant genotypes. Acta Hortic, 729:137–141.
  • [18] Salas, P., Prohens, J., Seguı´-Simarro, J.M. 2011. Evaluation of androgenic competence through anther culture in common eggplant and related species. Euphytica, 182:261–274.
  • [19] Salas, P., Rivas-Sendra, A., Prohens, J., Segui-Simarro, J.M. 2012. Influence of the stage for anther excision and heterostyly in embryogenesis induction from eggplant anther cultures. Euphytica, 184:235–250.
  • [20] Başay, S., Ellialtıoğlu, Ş. 2012. Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.). Turk J Biol, 37: 499-505.
  • [21] Gu, S.R. 1979. Plantlets from isolated pollen cultures of eggplant (Solanum melongena L.). Acta Bot Sin, 21: 30–36.
  • [22] Miyoshi, K. 1996. Callus induction and plantlet formation through culture of isolated microspores of eggplant (Solanum melongena L.). Plant Cell Rep, 15:391–395.
  • [23] Corral-Martinez, P., Segui-Simarro, J.M. 2012. Efficient production of callus-derived doubled haploids through isolated microspore culture in eggplant (Solanum melongena L.). Euphytica, 187:47–61.
  • [24] Corral-Martinez, P., Segui-Simarro, J.M. 2014. Refining the method for eggplant microspore culture: effect of abscisic acid, epibrassinolide, polyethylene glycol, naphthaleneacetic acid, 6-benzylaminopurine and arabinogalactan proteins. Euphytica, 195: 369–382.
  • [25] Bal, U., Abak, K. 2005. Induction of Symmetrical Nucleus Division and Multicellular Structures from the Isolated Microspores of Lycopersicon Esculentum Mill. Biotechnology & Biotechnological Equipment, 19(1): 35-42.
  • [26] Bal, U., Ellialtioglu, S., Abak, K. 2009. Induction of symmetrical nucleus division and multi-nucleate structures in microspores of eggplant (Solanum melongena L.) cultured in vitro. Sci Agric. 66: 535–539.
  • [27] Tuncer, B., Yanmaz, R. 2011a. Effects of colchicine and high temperature treatments on isolated microspore culture in various cabbage (Brassica oleraceae) types. International Journal of Agriculture and Biology, 13(5): 819-822.
  • [28] Tuncer, B., Yanmaz, R. 2011b. Induction of microspore embryogenesis in ornamental kale by gamma irradiation and high temperature stress. Asian Journal of Biotechnology, 3: 415-421.
  • [29] Tuncer, B., Cig, A., Yanmaz, R., Yasar, F. 2016. Effect of heat shock treatment on microspore embryogenesis in Brassica oleracea species. Journal of Agricultural Scieces, 22(4): 548-554.
  • [30] Tuncer, B. 2017. Callus formation from isolated microspore culture in radish (Raphanus sativus L.). The Journal of Animal & Plant Sciences, 27(1): 277-282.
  • [31] Custers, J.B.M. 2003. Microspore culture in rapeseed (Brassica napus L.). pp 185-194. Maluszynski, M., Kasha, K.J., Forster, B.P., Szarejko, I. Eds. 2003, Doubled haploid production in crop plants: a manual. Kluwer Academic Publishers, Dordrecht, Netherlands, 428.
  • [32] Touraev, A., Pfosser, M., Heberle-Bors, E. 2001. The microspore: a haploid multipurpose cell. Adv Bot Res, 35:53–109.
  • [33] Segui-Simarro, J.M., Nuez, F. 2008. How microspores transform into haploid embryos: changes associated with embryogenesis induction and microspore-derived embryogenesis. Physiologia Plantarum, 134: 1-12.
  • [34] Indrianto, A., Barinova, I., Touraev, A., Heberle-Bors, E. 2001. Tracking individual wheat microspores in vitro: identification of embryogenic microspores and body axis formation in the embryo. Planta, 212: 163–174.
  • [35] Maraschin, S.F., Vennik, M., Lamers, G.E.M., Spaink, H.P., Wang, M. 2005. Time-lapse tracking of barley androgenesis reveals position-determined cell death within pro-embryos. Planta, 220: 531–540.
  • [36] Zaki, M.A.M., Dickinson, H.G. 1991. Microspore-derived embryos in Brassica: the significance of division symmetry in pollen mitosis I to embryogenic development. Sexual Plant Reproduction, 4:48–55.
Toplam 36 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Mühendislik
Bölüm Makaleler
Yazarlar

Buse Çelik

Ahmet Naci Onus

Yayımlanma Tarihi 1 Mart 2019
Yayımlandığı Sayı Yıl 2019 Cilt: 23 Sayı: Özel

Kaynak Göster

APA Çelik, B., & Onus, A. N. (2019). Patlıcanda (Solanum melongena L.) Mikrospor Kültürü Üzerine Bir Ön Araştırma. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 23, 61-66. https://doi.org/10.19113/sdufenbed.431497
AMA Çelik B, Onus AN. Patlıcanda (Solanum melongena L.) Mikrospor Kültürü Üzerine Bir Ön Araştırma. SDÜ Fen Bil Enst Der. Mart 2019;23:61-66. doi:10.19113/sdufenbed.431497
Chicago Çelik, Buse, ve Ahmet Naci Onus. “Patlıcanda (Solanum Melongena L.) Mikrospor Kültürü Üzerine Bir Ön Araştırma”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 23, Mart (Mart 2019): 61-66. https://doi.org/10.19113/sdufenbed.431497.
EndNote Çelik B, Onus AN (01 Mart 2019) Patlıcanda (Solanum melongena L.) Mikrospor Kültürü Üzerine Bir Ön Araştırma. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 23 61–66.
IEEE B. Çelik ve A. N. Onus, “Patlıcanda (Solanum melongena L.) Mikrospor Kültürü Üzerine Bir Ön Araştırma”, SDÜ Fen Bil Enst Der, c. 23, ss. 61–66, 2019, doi: 10.19113/sdufenbed.431497.
ISNAD Çelik, Buse - Onus, Ahmet Naci. “Patlıcanda (Solanum Melongena L.) Mikrospor Kültürü Üzerine Bir Ön Araştırma”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 23 (Mart 2019), 61-66. https://doi.org/10.19113/sdufenbed.431497.
JAMA Çelik B, Onus AN. Patlıcanda (Solanum melongena L.) Mikrospor Kültürü Üzerine Bir Ön Araştırma. SDÜ Fen Bil Enst Der. 2019;23:61–66.
MLA Çelik, Buse ve Ahmet Naci Onus. “Patlıcanda (Solanum Melongena L.) Mikrospor Kültürü Üzerine Bir Ön Araştırma”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, c. 23, 2019, ss. 61-66, doi:10.19113/sdufenbed.431497.
Vancouver Çelik B, Onus AN. Patlıcanda (Solanum melongena L.) Mikrospor Kültürü Üzerine Bir Ön Araştırma. SDÜ Fen Bil Enst Der. 2019;23:61-6.

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