The Effect of Storage Conditions of Monofloral Honeys on Antimicrobial Activity

Year 2022, Volume: 26 Issue: 2, 299 - 304, 20.08.2022

Ayşe Sena Engin , Özgür Ceylan , Mehmet Emin Duru

https://doi.org/10.19113/sdufenbed.1062571

Cited By: 2

Abstract

This study aimed to determine the change of antimicrobial effects of sunflower, astragalus, chestnut and citrus honey against four bacteria (Listeria monocytogenes ATCC 7944, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Salmonella typhimurium ATCC 14028) and two yeasts (Candida albicans ATCC 10239 and Candida tropicalis ATCC 750) depending on storage conditions (heat and light applications) and time. Each monofloral honey was stored in two different conditions, with and without heat treatment, at room temperature (<25°C) in the light and in the dark. The antimicrobial activities of the maintained honey were determined by the disc diffusion method. At the end of the study, it was determined that all honeys showed the highest activity against S. typhimurium in conditions that did not see heat and light.

Keywords

Honey, Antimicobial activity, Heat treatment, Sunflower, Astragalus, Chestnut, Citrus

Monofloral Balların Saklama Koşullarına Göre Antimikrobiyal Aktivite Üzerine Etkisi

Abstract

Bu çalışmada ayçiçek, geven, kestane ve narenciye ballarının 4 bakteri (Listeria monocytogenes ATCC 7944, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Salmonella typhimurium ATCC 14028 ) ve 2 mayaya ( Candida albicans ATCC 10239 ve Candida tropicalis ATCC 750) karşı antimikrobiyal etkilerinin saklama koşulları (ısı ve ışık uygulamalarının) ile beraber zamana bağlı olarak değişimini belirlemek amaçlanmıştır. Her bir monofloral bal ısıl işlem yapılmış ve yapılmamış olarak, oda sıcaklığında (<25°C) ışık gören ve görmeyen şekilde iki farklı koşulda saklanmıştır. Muhafaza edilen balların antimikrobiyal aktiviteleri disk difüzyon yöntemi ile yapılmıştır. Yapılan çalışma sonunda balların en yüksek aktiviteyi S. typhimurium ‘a karşı ısı ve ışık görmeyen koşullarda gösterdiği belirlenmiştir.

Keywords

Bal, Antimikrobiyal aktivite Isıl İşlem, Ayçiçek, Geven, Kestane, Narenciye

References

• [1] Şahinler, N. 2000. Arı ürünleri ve insan sağlığı açısından önemi. MKÜ Ziraat Fakültesi Dergisi, 5(1-2), 139-148.
• [2] Aytuğ, B., Aykut, S., Merev, N., Edis, G. 1971. İstanbul Çevresi Bitkilerinin Polen Atlası İstanbul Üniversitesi Yayınları, 330 s.
• [3] Haffeejee, I. E., Moosa, A. 1985. Honey in the treatment of infantile gastroenteritis. British Medical Journal, 290, 1886-1887.
• [4] Ladas, S. P., Haritos, D. N., Raptis, S. A. 1995. Honey may have a laxative effect on normal subjects because of incomplete fructose absorption. The American Journal of Clinical Nutrition, 62, 1212-1215.
• [5] Effem, S. E. 1988. Clinical observations on the wound healing properties of honey. British Journal of Surgery, 75, 679-681.
• [6] Subrahmanyam, M. 1991. Topical application of honey in the treatment of burns. British Journal of Surgery, 78, 497-498.
• [7] Syazana, M. S. N., Halim, A. S., Gan, S. H., Shamsuddin, S. 2011. Antiproliferative effect of methanolic extraction of tualang honey on human keloid fibroblasts. BMC Complementary and Alternative Medicine, 11, 82.
• [8] Ali, A. T., Chowdhury, M. N., Al-Humayyd, M. S. 1991. Inhibitory effect of natural honey on helicobacter pylori. Tropical Gastroenterology. 12, 73-77.
• [9] Ali, A.T. 1995. Natural Honey Exerts Its Protective Effects Against Ethanol-Induced Gastric Lesions in Rats by Preventing Depletion of Glandular Nonprotein Sulfhydryls. Tropical Gastroenterology. 16, 18-26.
• [10] Biglari, B., Linden, P. H., Simon, A., Aytac, S., Gerner, H. J. Moghaddam, A. 2012. Use of medihoney as a non-surgical therapy for chronic pressure ulcers in patients with spinal cord injury. Spinal Cord, 50, 165-169.
• [11] Mundo, M. A., Padilla-Zakour, O. I., Worobo, R. W. 2004. Growht inhibition of foodborne pathogens and food spoilage organisms by select raw honeys. International Journal of Food Microbiology, 97, 1-8.
• [12] Isabel Martos, Moncef Cossentini, Federico Ferreres, Francisco A. Tomás-Barberán. 1997. Flavonoid composition of tunisian honeys and propolis. Journal of Agricultural and Food Chemistry. 45(8), 2824-2829.
• [13] Oszmianski, J., Lee, C. Y. 1990. Inhibition of polyphenol oxidase activity and browning by honey. Journal of Agricultural and Food Chemistry, 38, 1892–1895.
• [14] McKibben, J., Engeseth, N. J. 2002. Honey as a protective agent against lipid oxidation in ground Turkey. Journal of Agricultural and Food Chemistry, 50, 592–595.
• [15] Asadi-Pooya, A. A., Pnjehshahin, M. R., Beheshti, S. 2003. The antimycobacterial effect of honey:an in vitro study. Rivista di Biologia, 96(3), 491- 495.
• [16] Bansal, V., Medhi, B., Pandhi, P. 2005. Honey–a remedy rediscovered and its therapeutic utility. Kathmandu University Medical Journal, 3(3), 305–309.
• [17] Kolayli, S., Can, Z., Yildiz, O., Sahin, H., Karaoglu, S. A. 2016. A comparative study of the antihyaluronidase, antiurease, antioxidant, antimicrobial and physicochemical properties of different unifloral degrees of chestnut (Castanea sativa Mill.) honeys. Journal of Enzyme Inhibition and Medicinal Chemistry, 31(3), 96-104.
• [18] Ertürk, Ö., Şahin, H., Kolaylı, S., Ayvaz, M. Ç. 2014. Antioxidant and antimicrobial activity of East Black Sea Region honeys. Turkish Journal of Biochemistry, 39(1), 99-106.
• [19] Kucuk, M., Kolaylı, S., Karaoglu, S., Ulusoy, E., Baltacı, C., Candan, F. 2007. Biological activities and chemical composition of three honeys of different types from Anatolia. Food Chemistry, 100, 526–534.
• [20] Silici, S., Sagdic, O., Ekici, L. 2010. Total phenolic content, antiradical, antioxidant and antimicrobial activities of rhododendron honeys. Food Chemistry, 121, 238–243.
• [21] Alvarez-Suarez, J. M., Tulipani, S., Diaz, D., Estevez, Y., Romandini, S., Giampieri, F., Damiani, E., Astolfi, P., Bompadre, S., Battino, M. 2010. Antioxidant and antimicrobial capacity of several monofloral Cuban honeys and their correlation with color, polyphenol content and other chemical compounds. Food Chem Toxicology, 48, 2490–2499.
• [22] Al-Waili, N. S., Boni, N. S. 2003. Natural honey lowers plasma prostaglandin concentrations in normal individuals. Journal of Medicinal Food, 6(2), 129–133.
• [23] Clinical and Laboratory Standards Institute (CLSI), 2007. Methods for determining bactericidal activity of antimicrobial agents, tentative standard M26-T. National Committee for Clinical Laboratory Standards, Wayne, Philadelphia.
• [24] Irish, J., Carter, D. A., Shokohi, T., Blair, S. E. 2006. Honey has an antifungal effect against Candida species. Medical Mycology, 44(3), 289-291.
• [25] Mandal, M. D., Mandal, S. 2011. Honey: its medicinal property and antibacterial activity. Asian Pacific Journal of Tropical Biomedicine, 1(2), 154-160.
• [26] Taormina, P. J., Niemira, B. A., Beuchat, L. R. 2001. Inhibitory activity of honey against foodborne pathogens as influenced by the presence of hydrogen peroxide and level of antioxidant power. International Journal of Food Microbiology, 69(3), 217-225.
• [27] Gallardo-Chacón, J. J., Caselles, M., Izquierdo Pulido, M., Rius, N. 2008. Inhibitory activity of monofloral and multifloral honeys against bacterial pathogens. Journal of Apicultural Research, 47(2), 131-136.
• [28] Lee, H., Churey, J. J., Worobo, R. W. 2008. Purification and structural characterization of bacillomycin F produced by a bacterial honey isolate active against Byssochlamys fulva H25. Journal of Applied Microbiology, 105, 663–673.
• [29] Sagdic, O., Silici, S., Ekici, L. 2013. Evaluation of the phenolic content, antiradical, antioxidant, and antimicrobial activity of different floral sources of honey. International Journal of Food Properties, 16(3), 658-666.
• [30] Gulfraz, M., Ifftikhar, F., Asif, S., Raja, K. G., Asad, M. J., Imran, M., Abbasi, K., Zeenat, A. 2010. Quality assessment and antimicrobial activity of various honey types of Pakistan. African Journal of Biotechnology, 9(41), c-6906.