Baicaleinin İndirekt Seramik Restorasyonların Bağlanma Kuvvetine Etkisi

Abstract

Özet: Bu çalışmanın amacı, bir matriks metalloproteinaz inhibitörü olan Baicalein'in seramik simantasyonunda rezin-dentin bağlanma dayanımı üzerindeki etkisini değerlendirmektir. On altı insan mandibular yirmi yaş dişinin oklüzal minesi aşındırıldı ve cam matriks seramik (Celtra Duo) overleyler hazırlandı. Örnekler rastgele dört gruba ayrıldı. Kontrol grubu, total-etch dual-cure rezin siman (Variolink N) kullanılarak ek işlem yapılmadan simante edildi. Dimetil sülfoksit ve baicalein gruplarında asitleme işleminden sonra dentin yüzeyine ilgili gruba ait solüsyon uygulandı. Sonrasında seçilen simana ait rutin simantasyon işlemine devam edildi. Baicalein+ultrasonik aktivasyon grubunda diğer iki gruptan farklı olarak asitleme işleminden sonra baicalein solüsyonu uygulandı, 10 saniye boyunca ultrasonik aktivasyon yapıldı ve uygulanan materyallerin aynı penetrasyon derinliğine ulaşabilmesi için simantasyonun her aşamadasında ultrasonik aktivasyona devam edildi. Örnekler termal yaşlandırmaya tabi tutuldu. Örnekler kesilerek mikroçubuklar elde edildi ve mikroçekme testleri yapıldı. Bağ yüzeyi ve ön işlem görmüş oklüzal yüzey taramalı elektron mikroskobu kullanılarak görüntülendi. İstatistiksel analiz için Shapiro-Wilk testi, Kruskal-Wallis testi ve Dunn çoklu karşılaştırma testleri uygulandı. İstatistik analiz sonuçlarına göre kontrol grubunun bağlanma dayanımı değerleri diğer üç gruba oranla anlamlı derecede yüksek olduğu görüldü. Dimetil sülfoksit ve/veya baicalein solüsyonlarının kullanımı, total-etch dual-cure rezin siman için seramik ve dentine bağlanma dayanımını artırmadığı sonucuna varıldı.

Keywords

• MMP
• Degradasyon
• Mikroçekme testi

Effect of Baicalein on Bond Strength of Indirect Ceramic Restoration

Abstract

Abstract: This study aimed to evaluate the effect of a matrix metalloproteinase inhibitör (Baicalein) on the resin-dentin bond strength in ceramic cementation. The occlusal enamel of sixteen human mandibular wisdom teeth was cut, and glass matrix ceramic (Celtra Duo) overlays were prepared. The samples were randomly divided into four groups. The control group was cemented without pretreatment using total-etch dual-curing resin cement (Variolink N). In the dimethyl sulfoxide and baicalein groups, the solution was applied to the dentin after acid etching. Subsequently, the standard cementation process was continued. In the baicalein and ultrasonic agitation group, unlike the other two groups, after etching, the baicalein solution was applied, ultrasonic agitation was performed for 10 seconds, and agitation was carried out at each stage. The samples underwent thermal aging. Microbars were obtained by cutting the samples, and microtensile tests were conducted. The bond surface and the pretreated occlusal surface were observed using a scanning electron microscope. Shapiro-Wilk test, Kruskal-Wallis test and Dunn's multiple comparison test were applied for statistical analysis. The bond strength values of the control group were significantly higher than those of the other three groups. The use of dimethyl sulfoxide and/or baicalein solutions did not improve the bond strength to the ceramic and dentin for the total-etch dual-cure resin cement.

Keywords

• MMP
• Degradation
• Microtensile test

References

1. 1. Shenoy A, Shenoy NJ. Dental ceramics: an update. J Conserv Dent. 2010;13(4):195. doi:10.4103/0972-0707.73379.
2. 2. Gracis S, Thompson VP, Ferencz JL, Silva NR, Bonfante EA. A new classification system for all-ceramic and ceramic-like restorative materials. Int J Prosthodont. 2015;28(3):227-35. doi:10.11607/ijp.4244.
3. 3. Dentsply Sirona. Celtra Duo [Internet]. Charlotte (NC): Dentsply Sirona; [cited 2025 March 18]. Available from: https://www.dentsplysirona.com/en-us/discover/discover-by-brand/celtra-duo.html.
4. 4. Mazzoni A, Nascimento FD, Carrilho M, Tersariol I, Papa V, Tjäderhane L, et al. MMP activity in the hybrid layer detected with in situ zymography. J Dent Res. 2012;91(5):467-72. doi:10.1177/0022034512439210.
5. 5. Tjäderhane L, Nascimento FD, Breschi L, et al. Optimizing dentin bond durability: control of collagen degradation by matrix metalloproteinases and cysteine cathepsins. Dent Mater 2013;29(1):116-35 doi:101016/j.dental201208004.
6. 6. Rundhaug JE. Matrix metalloproteinases and angiogenesis. J Cell Mol Med. 2005;9(2):267-85. doi:10.1111/j.1582-4934.2005.tb00355.x.
7. 7. Cvikl B, Lussi A, Carvalho TS, Moritz A, Gruber R. Stannous chloride and stannous fluoride are inhibitors of matrix metalloproteinases. J Dent. 2018;78:51-8. doi:10.1016/j.jdent.2018.08.002.
8. 8. Jowkar Z, Firouzmandi M, Tabibi S. The effect of proanthocyanidin and casein phosphopeptide-amorphous calcium phosphate on the bond strength durability to caries-affected dentin. Clin Exp Dent Res. 2021;7(3):338-43. doi:10.1002/cre2.368.

9. 9. Frassetto A, Breschi L, Turco G, Marchesi G, Di Lenarda R, Tay FR, Pashley DH, Cadenaro M. Mechanisms of degradation of the hybrid layer in adhesive dentistry and therapeutic agents to improve bond durability: a literature review. Dent Mater 2016;32(2):e41-53 doi:101016/j.dental201511007.
10. 10. Lavanya A, Ramesh S, Kolaparthi VSK, Ch Nv M, Kvl SR, Mandava D. Natural polyphenols from Moringa and Centella extracts as collagen cross-linkers and matrix metalloproteinase (MMP) inhibitors in dentin preservation: an in vitro study. Cureus 2023 Nov;15(11):e48530 doi:107759/cureus48530.
11. 11. Bhandari S, Kondody RT, Nair AS, Mathew R, Divakar KP, Nambiar M. Evaluation of Aloe vera as matrix metalloproteinase inhibitor in human dentin with and without dentin-bonding agent: An in vitro study. J Conserv Dent 2021;24(5):491-495 doi:104103/jcd.jcd_474_21.
12. 12. Bie B, Sun J, Guo Y, et al. Baicalein: A review of its anti-cancer effects and mechanisms in hepatocellular carcinoma. Biomed Pharmacother. 2017;93:1285-91. doi:10.1016/j.biopha.2017.07.068.
13. 13. International Organization for Standardization. [Internet] ISO/TS 11405:2015. Dental materials—Testing of adhesion to tooth structure. Geneva: ISO; 2015 [cited 2025 Mar 18]. Available from: https://cdn.standards.iteh.ai/samples/62898/c8afc682cda547d797cf339c726837ce/ISO-TS-11405-2015.pdf
14. 14. Hashimoto M, Ohno H, Sano H, Kaga M, Oguchi H. Degradation patterns of different adhesives and bonding procedures. J Biomed Mater Res B Appl Biomater 2003;66(1):324-330 doi:101002/jbm.b10010.
15. 15. Lessa FC, Nogueira I, Huck C, Hebling J, Costa CA. Transdentinal cytotoxic effects of different concentrations of chlorhexidine gel applied on acid-conditioned dentin substrate. J Biomed Mater Res B Appl Biomater 2010;92(1):40-47 doi:101002/jbm.b31487.
16. 16. Schweikl H, Schmalz G. Glutaraldehyde-containing dentin bonding agents are mutagens in mammalian cells in vitro. J Biomed Mater Res. 1997;36(3):284-288.
17. 17. L. Kang, H. Yang, H. Yan, Y. Sun, X. Chen, J. Guo, J. Yue, H. Cui, Quercetin as a simple but versatile primer in dentin bonding, Rsc. Adv. 7(58) (2017) 36392-36402.
18. 18. L. Yi, J. Yu, L. Han, T. Li, H. Yang, C. Huang, Combination of baicalein and ethanol- wet-bonding improves dentin bonding durability, J. Dent. 90 (2019) 103207.
19. 19. D.J. Epasinghe, C.K. Yiu, M.F. Burrow, F.R. Tay, N.M. King, Effect of proanthocyanidin incorporation into dental adhesive resin on resin-dentine bond strength, J. Dent. 40(3) (2012) 173-80.
20. 20. Li J., Chen B., Hong N., Wu S., & Li Y. (2018). Effect of Baicalein on Matrix Metalloproteinases and Durability of Resin-Dentin Bonding. Operative Dentistry, 43(4), 426–436. doi:10.2341/17-097-l.
21. 21. Poyi Chua / Jiayan Lib / Wenting Liaoc / Shuyi Wud / Yan Lie. Effects of Baicalein on the Expression of Collagenolytic Enzymes in Human Dental Pulp Cells and Durability of Resin-Dentin Bondin. J Adhes Dent. 2019;(21:273–280).
22. 22. Mirzaei K, Ahmadi E, Rafeie N, Abbasi M. The effect of dentin surface pretreatment using dimethyl sulfoxide on the bond strength of a universal bonding agent to dentin. BMC Oral Health. 2023;23(1):250. Published 2023 Apr 29. doi:10.1186/s12903-023-02913-3.
23. 23. Syntac Primer Scientific Documentation. [Internet] Ivoclar Vivadent. [accessed 2025 Mar 18] Available from: https://ivodent.hu/__docs/796_a4617cb332e123213bd18d707397509c.pdf
24. 24. de Souza LC, Rodrigues NS, Cunha DA, et al. Two-year clinical evaluation of a proanthocyanidins-based primer in non-carious cervical lesions: A double-blind randomized clinical trial. J Dent. 2020;96:103325. doi:10.1016/j.jdent.2020.103325
25. 25. Choi CK, Son SA, Ha JH, Hur B, Kim HC, Kwon YH, et al. Influence of application methods of one-step self-etching adhesives on microtensile bond strength. J Korean Acad Conserv Dent. 2011;36(3):203-210. doi:10.5395/JKACD.2011.36.3.203
26. 26. Bagis B, Turkarslan S, Tezvergil-Mutluay A, Uctasli S, Vallittu PK, Lassila LV. Effect of ultrasonic agitation on bond strength of self-etching adhesives to dentin. J Adhes Dent. 2008;10(6):441-445.
27. 27. Bagis B, Turkaslan S, Vallittu PK, Lassila LV. Effect of high frequency ultrasonic agitation on the bond strength of self-etching adhesives. J Adhes Dent. 2009;11(5):369-374.
28. 28. Rahal V, de Oliveira FG, Briso AL, dos Santos PH, Sundefeld ML, Sundfeld RH. Correlation between hybrid layer thickness, resin tag length and microtensile bond strength of a self-etching adhesive system. Acta Odontol Latinoam. 2012;25(2):231-237.
29. 29. Anchieta RB, Oliveira FG, Sundfeld RH, et al. Analysis of hybrid layer thickness, resin tag length and their correlation with microtensile bond strength using a total etch adhesive to intact dentin. Acta Odontol Latinoam. 2011;24(3):272-278.
30. 30. Hahnel S, Henrich A, Bürgers R, Handel G, Rosentritt M. Investigation of mechanical properties of modern dental composites after artificial aging for one year. Oper Dent. 2010;35(4):412-419. doi:10.2341/09-337-L
31. 31. Fernandes VVB Jr, Oliani MG, Nogueira JMF Jr, Silva S, Araújo RM. Analysis and comparison of different bond strength tests. JSM Dent. 2016;4(5):1076.