Investigation of the effects of resveratrol on total oxidant/antioxidant capacity on experimental cigarette smoking model

Year 2015, Volume: 6 Issue: 1, 10 - 14, 27.04.2015

Özge Kolkesen Şahin Müge Çına Aksoy Efkan Uz Birsen Harun Dağdeviren

Abstract

AIM
The harmful effects of smoking on body are known to be caused by oxidative stress in tissues. With the many harmful substances in smoke it serve as a good model of free radical damage could be monitored. The agents preventing free radical induced oxidation, capable of trapping and stabilizing free radicals are called 'antioxidants". Resveratrol is a powerful antioxidant that has anti-inflammatory, analgesic and osteogenic effects. In this study we evaluated the effects of smoking and resveratrol on oxidative stress.
MATERIAL VE METHOD
For 4 weeks rats were inhalated cigarette smoke (6 cigarette/day) and after that time 20 mg/kg/day resveratrol was given via oral gavage during 28 days together with cigarette smoke. At the end of 8 weeks total exposure to cigarette smoke, rats were sacrified and bloods and tongue samples were collected. Biochemically serum and tissue total antioxidant / oxidant capacity (TAC / TOC) were evaluated.
RESULTS
There were no statistical difference between groups in terms of TAC and TOC serum. TAC tongue is higher in cigarette+resveratrol and cigarette groups.
CONCLUSION
In the experiment performed with 6 cigarettes per day for 8 weeks, toxic effect of cigarette induced antioxidant system in the organism to protect the body and that is more effective than antioxidant effect of resveratrol.

Keywords

Smoking, resveratrol, total oxidant capacity, total antioxidant capacity

Deneysel sigara modelinde resveratrolün total oksidan/antioksidan kapasite üzerine etkilerinin araştırılması

Abstract

AMAÇ
Sigaranın vücutta hasara neden olan etkilerinin dokularda oluşturduğu oksidatif strese bağlı olduğu bilinmektedir. Sigara içerdiği çok sayıdaki zararlı madde ile serbest radikal hasarının izlenebileceği bir model oluşturmaktadır. Serbest radikallerin neden olduğu oksidasyonları önleyen, serbest radikalleri yakalama ve stabilizasyon yeteneğine sahip maddelere 'antioksidan' adı verilir. Resveratrol antienflamatuar, osteojenik ve analjezik etkili güçlü bir antioksidandır. Bu çalışmada sigaranın neden olduğu oksidatif stres üzerine resvaratrolün etkisi araştırılmıştır.
MATERYAL VE METOD
Ratlar 4 hafta süresince sigara dumanına (6 sigara/gün) maruz bırakıldıktan sonra 28 gün boyunca 20 mg/kg/gün resveratrol intragastrik gavaj yoluyla verilmiştir. Sekiz haftalık deney süresi sonunda ratlar sakrifiye edilerek kan ve dil dokuları alınmıştır. Biyokimyasal olarak serum ve dokuda Total antioksidan/oksidan kapasite (TAK/TOK) değerlendirilmiştir.
BULGULAR
Serum TAK ve TOK açısından gruplar arasında anlamlı fark bulunmamıştır. TAK ve TOK dil sigara+resveratrol grubunda yüksek bulunmuştur.
SONUÇ
8 hafta süresince günde 6 adet sigarayla yapılan deneyde, sigaranın toksik etkisine karşı vücudun kendini korumak için aktive olan antioksidan sistemin etkisinin, resveratrolün antioksidan kapasitesinden daha yüksek olduğu sonucuna varılmıştır.

Keywords

Sigara, resveratrol, total oksidan kapasite, total antioksidan tapasite

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