Effect of Ascorbic Acid on SH-SY5Y Cells at Different pH Levels

Okan Sancer; Pınar Aslan Koşar

doi:10.17343/sdutfd.1614378

Effect of Ascorbic Acid on SH-SY5Y Cells at Different pH Levels

Abstract

Objective: Cancer is a complex disease, and many different methods are used to treat it. Ascorbic acid is one of the nutritional supplements used to prevent the proliferation of cancer cells. Therefore, the effect of ascorbic acid at different pH levels on SH-SY5Y cells was investigated. Material and Method: The cytotoxic effect of ascorbic acid at different pH levels and concentrations, and its effect on cell proliferation and gene expression were studied on SH-SY5Y cells. Results: The pH and ascorbic acid levels that decreased the viability of the SH-SY5Y cell line and inhibited migration in wound healing were pH:8 and IC50:4.15. In addition, it was determined that p53 expression level increased (p<0.05) and MDM2 and AKT1 expression levels decreased (p<0.05) at the pH and IC50 values mentioned above. Conclusion: The findings of this study show that AA applied at high pH affects the viability of SH-SY5Y cells, inhibits their migration capacity, and alters the expression levels of p53, MDM2, and AKT1 genes.

Keywords

Ethical Statement

Not applicable.

References

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Details

Primary Language

English

Subjects

Cell Metabolism, Gene Expression, Neurogenetics

Journal Section

Research Article

Authors

Okan Sancer ^*
0000-0001-7935-5004
Türkiye

Pınar Aslan Koşar
0000-0003-2602-5145
Türkiye

Early Pub Date

March 27, 2025

Publication Date

March 27, 2025

Submission Date

January 6, 2025

Acceptance Date

March 23, 2025

Published in Issue

Year 2025 Volume: 32 Number: 1

DOI

https://doi.org/10.17343/sdutfd.1614378

IZ

https://izlik.org/JA75XZ79ZS

Cite

RIS / Bibtex

APA

Sancer, O., & Aslan Koşar, P. (2025). Effect of Ascorbic Acid on SH-SY5Y Cells at Different pH Levels. Medical Journal of Süleyman Demirel University, 32(1), 89-94. https://doi.org/10.17343/sdutfd.1614378

AMA

1.Sancer O, Aslan Koşar P. Effect of Ascorbic Acid on SH-SY5Y Cells at Different pH Levels. Med J SDU. 2025;32(1):89-94. doi:10.17343/sdutfd.1614378

Chicago

Sancer, Okan, and Pınar Aslan Koşar. 2025. “Effect of Ascorbic Acid on SH-SY5Y Cells at Different PH Levels”. Medical Journal of Süleyman Demirel University 32 (1): 89-94. https://doi.org/10.17343/sdutfd.1614378.

EndNote

Sancer O, Aslan Koşar P (March 1, 2025) Effect of Ascorbic Acid on SH-SY5Y Cells at Different pH Levels. Medical Journal of Süleyman Demirel University 32 1 89–94.

IEEE

[1]O. Sancer and P. Aslan Koşar, “Effect of Ascorbic Acid on SH-SY5Y Cells at Different pH Levels”, Med J SDU, vol. 32, no. 1, pp. 89–94, Mar. 2025, doi: 10.17343/sdutfd.1614378.

ISNAD

Sancer, Okan - Aslan Koşar, Pınar. “Effect of Ascorbic Acid on SH-SY5Y Cells at Different PH Levels”. Medical Journal of Süleyman Demirel University 32/1 (March 1, 2025): 89-94. https://doi.org/10.17343/sdutfd.1614378.

JAMA

1.Sancer O, Aslan Koşar P. Effect of Ascorbic Acid on SH-SY5Y Cells at Different pH Levels. Med J SDU. 2025;32:89–94.

MLA

Sancer, Okan, and Pınar Aslan Koşar. “Effect of Ascorbic Acid on SH-SY5Y Cells at Different PH Levels”. Medical Journal of Süleyman Demirel University, vol. 32, no. 1, Mar. 2025, pp. 89-94, doi:10.17343/sdutfd.1614378.

Vancouver

1.Okan Sancer, Pınar Aslan Koşar. Effect of Ascorbic Acid on SH-SY5Y Cells at Different pH Levels. Med J SDU. 2025 Mar. 1;32(1):89-94. doi:10.17343/sdutfd.1614378