Effect of Ascorbic Acid on SH-SY5Y Cells at Different pH Levels

Okan Sancer; Pınar Aslan Koşar

doi:10.17343/sdutfd.1614378

Effect of Ascorbic Acid on SH-SY5Y Cells at Different pH Levels

Öz

Objective: Cancer is a complex disease, and many different methods are used to treat it. Ascorbic acid is one of the nutritional supplements used to prevent the proliferation of cancer cells. Therefore, the effect of ascorbic acid at different pH levels on SH-SY5Y cells was investigated. Material and Method: The cytotoxic effect of ascorbic acid at different pH levels and concentrations, and its effect on cell proliferation and gene expression were studied on SH-SY5Y cells. Results: The pH and ascorbic acid levels that decreased the viability of the SH-SY5Y cell line and inhibited migration in wound healing were pH:8 and IC50:4.15. In addition, it was determined that p53 expression level increased (p<0.05) and MDM2 and AKT1 expression levels decreased (p<0.05) at the pH and IC50 values mentioned above. Conclusion: The findings of this study show that AA applied at high pH affects the viability of SH-SY5Y cells, inhibits their migration capacity, and alters the expression levels of p53, MDM2, and AKT1 genes.

Anahtar Kelimeler

Etik Beyan

Not applicable.

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Hücre Metabolizması, Gen İfadesi, Nörogenetik

Bölüm

Araştırma Makalesi

Yazarlar

Okan Sancer ^*
0000-0001-7935-5004
Türkiye

Pınar Aslan Koşar
0000-0003-2602-5145
Türkiye

Erken Görünüm Tarihi

27 Mart 2025

Yayımlanma Tarihi

27 Mart 2025

Gönderilme Tarihi

6 Ocak 2025

Kabul Tarihi

23 Mart 2025

Yayımlandığı Sayı

Yıl 2025 Cilt: 32 Sayı: 1

DOI

https://doi.org/10.17343/sdutfd.1614378

IZ

https://izlik.org/JA75XZ79ZS

Kaynak Göster

RIS / Bibtex

APA

Sancer, O., & Aslan Koşar, P. (2025). Effect of Ascorbic Acid on SH-SY5Y Cells at Different pH Levels. Medical Journal of Süleyman Demirel University, 32(1), 89-94. https://doi.org/10.17343/sdutfd.1614378

AMA

1.Sancer O, Aslan Koşar P. Effect of Ascorbic Acid on SH-SY5Y Cells at Different pH Levels. SDÜ Tıp Fak Derg. 2025;32(1):89-94. doi:10.17343/sdutfd.1614378

Chicago

Sancer, Okan, ve Pınar Aslan Koşar. 2025. “Effect of Ascorbic Acid on SH-SY5Y Cells at Different pH Levels”. Medical Journal of Süleyman Demirel University 32 (1): 89-94. https://doi.org/10.17343/sdutfd.1614378.

EndNote

Sancer O, Aslan Koşar P (01 Mart 2025) Effect of Ascorbic Acid on SH-SY5Y Cells at Different pH Levels. Medical Journal of Süleyman Demirel University 32 1 89–94.

IEEE

[1]O. Sancer ve P. Aslan Koşar, “Effect of Ascorbic Acid on SH-SY5Y Cells at Different pH Levels”, SDÜ Tıp Fak Derg, c. 32, sy 1, ss. 89–94, Mar. 2025, doi: 10.17343/sdutfd.1614378.

ISNAD

Sancer, Okan - Aslan Koşar, Pınar. “Effect of Ascorbic Acid on SH-SY5Y Cells at Different pH Levels”. Medical Journal of Süleyman Demirel University 32/1 (01 Mart 2025): 89-94. https://doi.org/10.17343/sdutfd.1614378.

JAMA

1.Sancer O, Aslan Koşar P. Effect of Ascorbic Acid on SH-SY5Y Cells at Different pH Levels. SDÜ Tıp Fak Derg. 2025;32:89–94.

MLA

Sancer, Okan, ve Pınar Aslan Koşar. “Effect of Ascorbic Acid on SH-SY5Y Cells at Different pH Levels”. Medical Journal of Süleyman Demirel University, c. 32, sy 1, Mart 2025, ss. 89-94, doi:10.17343/sdutfd.1614378.

Vancouver

1.Okan Sancer, Pınar Aslan Koşar. Effect of Ascorbic Acid on SH-SY5Y Cells at Different pH Levels. SDÜ Tıp Fak Derg. 01 Mart 2025;32(1):89-94. doi:10.17343/sdutfd.1614378