Neuroprotective Effects of Dexpanthenol Against Chest Trauma-Induced Brain Injury in Rats

Şerife Taşan; Sanem Aşcı; Halil Aşçı

doi:10.17343/sdutfd.1629663

Neuroprotective Effects of Dexpanthenol Against Chest Trauma-Induced Brain Injury in Rats

Abstract

Objective: Chest trauma-induced brain injury (CTB) occurs as a result of the formation of inflammatory markers in the lung and blood. Vitamin B5, derived from dexpanthenol (DEX), has antioxidant, anti-inflammatory, and antiapoptotic properties. This study aimed to investigate the protective effects of DXP on CTB. Materials and methods: Forty Wistar Albino males were divided into four groups as sham, CTB (Dropping a 200g weight from a height of 1 meter onto the anterior chest wall), CTB+DXP (500mg/kg, ip), and DXP. After 48 hours, rats were sacrificed under anesthesia, and the brain tissues were put into 10% formaldehyde solution for histopathological and immunohistochemical examination. Results: In the CTB group, rats exhibited significant hemorrhage, increased TNF-α, Cas-3, and decreased MBP expressions in the brain compared to the control group. DXP treatment significantly reducted hemorrhage areas and reversed immunoexpressions. Conclusion: CTB may develop in brain tissue by causing inflammation, apoptosis, and myelin sheath damage. These adverse effects can be reversed with DXP treatment.

Keywords

Ethical Statement

In this study, all experiments were performed under the guidelines for animal research from the National Institutes of Health and were approved by the Committee on Animal Research of Suleyman Demirel University, Isparta (Ethic No:09.05.2024/300).

References

1. Požgain Z, Kristek D, Lovrić I, Kondža G, Jelavić M, Kocur J, et al. Pulmonary contusions after blunt chest trauma: clinical significance and evaluation of patient management. Eur J Trauma Emerg Surg 2018;44:773–7.
2. Lewis BT, Herr KD, Hamlin SA, Henry T, Little BP, Naeger DM, et al. Imaging manifestations of chest trauma. Radiographics 2021;41(5):1321–34.
3. Mert Ü, Andruszkow H, Hildebrand F. Chest trauma: Classification and influence on the general management. In: Textbook of Polytrauma Management: A Multidisciplinary Approach. Cham: Springer International Publishing; 2022. p. 161–84.
4. Bezerra FS, Lanzetti M, Nesi RT, Nagato AC, Silva CPE, Kennedy-Feitosa E, et al. Oxidative stress and inflammation in acute and chronic lung injuries. Antioxidants 2023;12(3):548.
5. Di Bella D, Ferreira JP, Silva RDNO, Echem C, Milan A, Akamine EH, et al. Gold nanoparticles reduce inflammation in cerebral microvessels of mice with sepsis. J Nanobiotechnol 2021;19:1–1.
6. Li R, Ye JJ, Gan L, Zhang M, Sun D, Li Y, Wang T, Chang P. Traumatic inflammatory response: pathophysiological role and clinical value of cytokines. Eur J Trauma Emerg Surg 2024;50(4):1313-1330.
7. Hazeldine J, Foster M. The immune and inflammatory response to major traumatic injury. In: Blast Injury Science and Engineering: A Guide for Clinicians and Researchers. Cham: Springer International Publishing; 2023. p. 14.
8. Walsh SA, Hoyt BW, Rowe CJ, Dey D, Davis TA. Alarming Cargo: The Role of Exosomes in Trauma-Induced Inflammation. Biomolecules 2021;31;11(4):522.

9. Smirnova EV, Rakitina TV, Ziganshin RH, et al. Comprehensive atlas of the myelin basic protein ınteraction landscape. Biomolecules 2021;3;11(11):1628.
10. Shenfeld A, Galkin A. Role of the MBP protein in myelin formation and degradation in the brain. Bio. Comm 2022;67(2): 127–13.
11. Shang Y, Wang Y, Guo Y, Ren L, Zhang X, Wang S, et al. Analysis of the risk of traumatic brain injury and evaluation of neurogranin and myelin basic protein as potential biomarkers of traumatic brain injury in postmortem examination. Forensic Sci Med Pathol 2022;18(3).
12. Karakuyu NF, Özmen Ö. Dexpanthenol Inhibits ınflammation and apoptosis in LPS-ınduced acute lung ınjury by reducing ıncreased VCAM-1 and caspase-3 expressions in rats. KVJ 2022;15(3):303-10.
13. Zhou Y, Fan R, Botchway BO, Zhang Y, Liu X. Infliximab can improve traumatic brain injury by suppressing the tumor necrosis factor alpha pathway. Mol Neurobiol 2021;58(6):2803–11.
14. Gu M, Mei XL, Zhao YN. Sepsis and cerebral dysfunction: BBB damage, neuroinflammation, oxidative stress, apoptosis, and autophagy as key mediators and the potential therapeutic approaches. Neurotox Res 2021;39:489–503.
15. Zhou R, Sun X, Li Y, Huang Q, Qu Y, Mu D, et al. Low-dose dexamethasone increases autophagy in cerebral cortical neurons of juvenile rats with sepsis associated encephalopathy. Neuroscience 2019;419:83–99.
16. Graff-Radford J, Lesnick T, Rabinstein AA, Gunter J, Aakre J, Przybelski SA, et al. Cerebral microbleed incidence, relationship to amyloid burden: The Mayo Clinic Study of Aging. Neurology 2020;94(2):e190–e199.
17. Karatoprak DE, Engin R, Sahin S, İclek İ, Durak MA. Investigation of neuroprotective efficacy of dexpanthenol in an experimental head injury model. J Korean Neurosurg Soc 2024.
18. Gülmez A, Bektaşoğlu PK, Tönge Ç, Yaprak A, Türkoğlu ME, Önder E, et al. Neuroprotective effects of dexpanthenol on rabbit spinal cord ischemia/reperfusion injury model. World Neurosurg 2022;167:e172–e183.
19. Raghavendran K, Davidson BA, Helinski JD, Marschke CJ, Manderscheid P, Woytash JA, et al. A rat model for isolated bilateral lung contusion from blunt chest trauma. Anesthesia & Analgesia 2005;101(5):1482-9.
20. Mielke D, Bleuel K, Stadelmann C, et al. The ESAS-score: A histological severity grading system of subarachnoid hemorrhage using the modified double hemorrhage model in rats. PLoS One 2020;15(2), e0227349.
21. Dogrul BN, Kiliccalan I, Asci ES, Peker SC. Blunt trauma related chest wall and pulmonary injuries: An overview. Chin J Traumatol 2020;23(3):125–38.
22. Oestreich MA, Seidel K, Bertrams W, Müller HH, Sassen M, Steinfeldt T, et al. Pulmonary inflammatory response and immunomodulation to multiple trauma and hemorrhagic shock in pigs. PLoS One 2022;17(12):e0278766.
23. Noorbakhsh MR, Kriley IR. Management of severe respiratory failure in complex trauma patients. J Emerg Crit Care Med 2018;2(3).
24. Xu J, Zhan T, Zheng WK, Huang YK, Chen K, Zhang XH, et al. Hydroxysafflor yellow A acutely attenuates blood-brain barrier permeability, oxidative stress, inflammation, and apoptosis in traumatic brain injury in rats. Acta Cir Bras 2021;35:e351202.
25. Huang X, Hussain B, Chang J. Peripheral inflammation and blood-brain barrier disruption: Effects and mechanisms. CNS Neurosci Ther 2021;27(1):36–47.
26. Qin D, Wang J, Le A, Wang TJ, Chen X, Wang J. Traumatic brain injury: Ultrastructural features in neuronal ferroptosis, glial cell activation and polarization, and blood-brain barrier breakdown. Cells 2021;10(5):1009.
27. Kose A, Parlakpinar H, Ozhan O, Ermis N, Yildiz A, Vardi N, et al. Therapeutic effects of dexpanthenol on the cardiovascular and respiratory systems following cecal ligation and puncture-induced sepsis in rats. Biotechnic Histochem 2020;95(6):428–37.

Details

Primary Language

English

Subjects

Health Services and Systems (Other)

Journal Section

Research Article

Authors

Şerife Taşan ^*
0000-0002-1469-3464
Türkiye

Sanem Aşcı
0000-0002-1283-2096
Türkiye

Halil Aşçı
0000-0002-1545-035X
Türkiye

Publication Date

September 9, 2025

Submission Date

January 30, 2025

Acceptance Date

June 11, 2025

Published in Issue

Year 2025 Volume: 32 Number: 3

DOI

https://doi.org/10.17343/sdutfd.1629663

IZ

https://izlik.org/JA84MM49EE

Cite

RIS / Bibtex

APA

Taşan, Ş., Aşcı, S., & Aşçı, H. (2025). Neuroprotective Effects of Dexpanthenol Against Chest Trauma-Induced Brain Injury in Rats. Medical Journal of Süleyman Demirel University, 32(3), 184-190. https://doi.org/10.17343/sdutfd.1629663

AMA

1.Taşan Ş, Aşcı S, Aşçı H. Neuroprotective Effects of Dexpanthenol Against Chest Trauma-Induced Brain Injury in Rats. Med J SDU. 2025;32(3):184-190. doi:10.17343/sdutfd.1629663

Chicago

Taşan, Şerife, Sanem Aşcı, and Halil Aşçı. 2025. “Neuroprotective Effects of Dexpanthenol Against Chest Trauma-Induced Brain Injury in Rats”. Medical Journal of Süleyman Demirel University 32 (3): 184-90. https://doi.org/10.17343/sdutfd.1629663.

EndNote

Taşan Ş, Aşcı S, Aşçı H (September 1, 2025) Neuroprotective Effects of Dexpanthenol Against Chest Trauma-Induced Brain Injury in Rats. Medical Journal of Süleyman Demirel University 32 3 184–190.

IEEE

[1]Ş. Taşan, S. Aşcı, and H. Aşçı, “Neuroprotective Effects of Dexpanthenol Against Chest Trauma-Induced Brain Injury in Rats”, Med J SDU, vol. 32, no. 3, pp. 184–190, Sept. 2025, doi: 10.17343/sdutfd.1629663.

ISNAD

Taşan, Şerife - Aşcı, Sanem - Aşçı, Halil. “Neuroprotective Effects of Dexpanthenol Against Chest Trauma-Induced Brain Injury in Rats”. Medical Journal of Süleyman Demirel University 32/3 (September 1, 2025): 184-190. https://doi.org/10.17343/sdutfd.1629663.

JAMA

1.Taşan Ş, Aşcı S, Aşçı H. Neuroprotective Effects of Dexpanthenol Against Chest Trauma-Induced Brain Injury in Rats. Med J SDU. 2025;32:184–190.

MLA

Taşan, Şerife, et al. “Neuroprotective Effects of Dexpanthenol Against Chest Trauma-Induced Brain Injury in Rats”. Medical Journal of Süleyman Demirel University, vol. 32, no. 3, Sept. 2025, pp. 184-90, doi:10.17343/sdutfd.1629663.

Vancouver

1.Şerife Taşan, Sanem Aşcı, Halil Aşçı. Neuroprotective Effects of Dexpanthenol Against Chest Trauma-Induced Brain Injury in Rats. Med J SDU. 2025 Sep. 1;32(3):184-90. doi:10.17343/sdutfd.1629663