Resveratrol ve Dialil Disülfitin Sıçan Testisinde Kadmiyum ile Oluşturulmuş Akut Hasara Etkisi

Kıymet Halk; Zeynep Kahveci; İlkin Çavuşoğlu; Şahin Sırmalı

doi:10.17343/sdutfd.554038

Resveratrol ve Dialil Disülfitin Sıçan Testisinde Kadmiyum ile Oluşturulmuş Akut Hasara Etkisi

Abstract

Bu çalışmada, önemli bir çevre kirletici ve dokular üzerine zararlı etkileri olan kadmiyumun erkek fertilitesi üzerine oluşturduğu hasara karşı resveratrolün ve dialil disülfitin koruyucu etkileri araştırıldı.

Yapılan deneyde Wistar albino cinsi rat kullanıldı ve 5 grup (A: kontrol, B1: CdCl₂, B2: RES + CdCl₂, B3: DDS + CdCl₂, B4: RES + DDS + CdCl₂) oluşturuldu. Histolojik değişiklikleri değerlendirmek için testis dokuları, Hematoksilen ve Eozin ile boyandı ve Modifiye Johnsen skorlaması yapıldı. Gruplarda gözlenen apoptoz, TUNEL yöntemi ve aktif kaspaz-3 immunohistokimyası ile değerlendirildi.

Elde ettiğimiz bulgulara göre, B1 grubunda, kadmiyumun seminifer tübüllerde hasara yol açtığı, spermatogenezi durdurduğu ve germ hürcelerinde apoptozu indüklediği gözlendi. Modifiye Johnsen skorlama sonuçlarında kontrole göre B1 grubunda anlamlı derecede azalma, apoptotik indeks sonuçlarında ise anlamlı derecede artış görüldü. B1 grubuyla B2, B3 ve B4 grupları karşılaştırıldığında; Modifiye Johnsen skorlama sonuçlarında B1 ile B2 grubu arasında anlamlı bir farklılık görülmezken, B3 ve B4 gruplarında anlamlı artış görüldü. Ayrıca apoptotik indeks sonuçlarında B2 grubunda anlamlı bir farklılık görülmezken, B3 ve B4 gruplarında anlamlı azalma görüldü.

Sonuç olarak, sıçan testisinde kadmiyum ile oluşturulan akut hasarın baskılanmasında resveratrol ve dialil disülfitin rolüne yönelik bilgiler ortaya konuldu. Belirlediğimiz dozda resveratrol bu akut hasarın baskılanmasında etkili olmazken dialil disülfit ve resveratrol ile birlikte kullanımı hasarı önlemede etkili bulundu. Literatür analizimize göre bu çalışma kadmiyumun indüklediği testis hasarına karşı dialil disülfitin koruyucu etkilerinin gösterildiği ilk çalışmadır.

Keywords

References

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Details

Primary Language

Turkish

Subjects

Clinical Sciences

Journal Section

Research Article

Authors

Kıymet Halk ^*
Türkiye

Zeynep Kahveci This is me
Türkiye

İlkin Çavuşoğlu
Türkiye

Şahin Sırmalı This is me
Türkiye

Publication Date

September 1, 2020

Submission Date

May 2, 2019

Acceptance Date

June 28, 2019

Published in Issue

Year 2020 Volume: 27 Number: 3

DOI

https://doi.org/10.17343/sdutfd.554038

IZ

https://izlik.org/JA24CL62PT

Cite

RIS / Bibtex

APA

Halk, K., Kahveci, Z., Çavuşoğlu, İ., & Sırmalı, Ş. (2020). Resveratrol ve Dialil Disülfitin Sıçan Testisinde Kadmiyum ile Oluşturulmuş Akut Hasara Etkisi. Medical Journal of Süleyman Demirel University, 27(3), 273-282. https://doi.org/10.17343/sdutfd.554038

AMA

1.Halk K, Kahveci Z, Çavuşoğlu İ, Sırmalı Ş. Resveratrol ve Dialil Disülfitin Sıçan Testisinde Kadmiyum ile Oluşturulmuş Akut Hasara Etkisi. Med J SDU. 2020;27(3):273-282. doi:10.17343/sdutfd.554038

Chicago

Halk, Kıymet, Zeynep Kahveci, İlkin Çavuşoğlu, and Şahin Sırmalı. 2020. “Resveratrol Ve Dialil Disülfitin Sıçan Testisinde Kadmiyum Ile Oluşturulmuş Akut Hasara Etkisi”. Medical Journal of Süleyman Demirel University 27 (3): 273-82. https://doi.org/10.17343/sdutfd.554038.

EndNote

Halk K, Kahveci Z, Çavuşoğlu İ, Sırmalı Ş (September 1, 2020) Resveratrol ve Dialil Disülfitin Sıçan Testisinde Kadmiyum ile Oluşturulmuş Akut Hasara Etkisi. Medical Journal of Süleyman Demirel University 27 3 273–282.

IEEE

[1]K. Halk, Z. Kahveci, İ. Çavuşoğlu, and Ş. Sırmalı, “Resveratrol ve Dialil Disülfitin Sıçan Testisinde Kadmiyum ile Oluşturulmuş Akut Hasara Etkisi”, Med J SDU, vol. 27, no. 3, pp. 273–282, Sept. 2020, doi: 10.17343/sdutfd.554038.

ISNAD

Halk, Kıymet - Kahveci, Zeynep - Çavuşoğlu, İlkin - Sırmalı, Şahin. “Resveratrol Ve Dialil Disülfitin Sıçan Testisinde Kadmiyum Ile Oluşturulmuş Akut Hasara Etkisi”. Medical Journal of Süleyman Demirel University 27/3 (September 1, 2020): 273-282. https://doi.org/10.17343/sdutfd.554038.

JAMA

1.Halk K, Kahveci Z, Çavuşoğlu İ, Sırmalı Ş. Resveratrol ve Dialil Disülfitin Sıçan Testisinde Kadmiyum ile Oluşturulmuş Akut Hasara Etkisi. Med J SDU. 2020;27:273–282.

MLA

Halk, Kıymet, et al. “Resveratrol Ve Dialil Disülfitin Sıçan Testisinde Kadmiyum Ile Oluşturulmuş Akut Hasara Etkisi”. Medical Journal of Süleyman Demirel University, vol. 27, no. 3, Sept. 2020, pp. 273-82, doi:10.17343/sdutfd.554038.

Vancouver

1.Kıymet Halk, Zeynep Kahveci, İlkin Çavuşoğlu, Şahin Sırmalı. Resveratrol ve Dialil Disülfitin Sıçan Testisinde Kadmiyum ile Oluşturulmuş Akut Hasara Etkisi. Med J SDU. 2020 Sep. 1;27(3):273-82. doi:10.17343/sdutfd.554038