Resveratrol ve Dialil Disülfitin Sıçan Testisinde Kadmiyum ile Oluşturulmuş Akut Hasara Etkisi

Year 2020, , 273 - 282, 01.09.2020

Kıymet Halk , Zeynep Kahveci İlkin Çavuşoğlu , Şahin Sırmalı

https://doi.org/10.17343/sdutfd.554038

Abstract

Bu çalışmada, önemli bir çevre
kirletici ve dokular üzerine zararlı etkileri olan kadmiyumun erkek fertilitesi
üzerine oluşturduğu hasara karşı resveratrolün ve dialil disülfitin koruyucu
etkileri araştırıldı.

Yapılan deneyde Wistar albino cinsi
rat kullanıldı ve 5 grup (A: kontrol, B1: CdCl₂, B2: RES + CdCl₂,
B3: DDS + CdCl₂, B4: RES + DDS + CdCl₂) oluşturuldu.
Histolojik değişiklikleri değerlendirmek için testis dokuları, Hematoksilen ve
Eozin ile boyandı ve Modifiye Johnsen skorlaması yapıldı. Gruplarda gözlenen
apoptoz, TUNEL yöntemi ve aktif kaspaz-3 immunohistokimyası ile
değerlendirildi.

Elde ettiğimiz bulgulara göre, B1
grubunda, kadmiyumun seminifer tübüllerde hasara yol açtığı, spermatogenezi
durdurduğu ve germ hürcelerinde apoptozu indüklediği gözlendi. Modifiye Johnsen
skorlama sonuçlarında kontrole göre B1 grubunda anlamlı derecede azalma,
apoptotik indeks sonuçlarında ise anlamlı derecede artış görüldü. B1 grubuyla
B2, B3 ve B4 grupları karşılaştırıldığında; Modifiye Johnsen skorlama
sonuçlarında B1 ile B2 grubu arasında anlamlı bir farklılık görülmezken, B3 ve
B4 gruplarında anlamlı artış görüldü. Ayrıca apoptotik indeks sonuçlarında B2
grubunda anlamlı bir farklılık görülmezken, B3 ve B4 gruplarında anlamlı azalma
görüldü.

Sonuç olarak, sıçan testisinde
kadmiyum ile oluşturulan akut hasarın baskılanmasında resveratrol ve dialil
disülfitin rolüne yönelik bilgiler ortaya konuldu. Belirlediğimiz dozda
resveratrol bu akut hasarın baskılanmasında etkili olmazken dialil disülfit ve
resveratrol ile birlikte kullanımı hasarı önlemede etkili bulundu. Literatür
analizimize göre bu çalışma kadmiyumun indüklediği testis hasarına karşı dialil
disülfitin koruyucu etkilerinin gösterildiği ilk çalışmadır.

Keywords

Kadmiyum, resveratrol, dialil disülfit, sıçan testis hasarı, apoptoz

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