Relationship between Tau-fluvalinate Resistance and Carboxylesterase Enzyme in Myzus persicae (Hemiptera: Aphididae) Populations

Abstract

Myzus persicae is an important pest that causes significant economic losses in various crops. Worldwide, control of Myzus persicae largely relies on the use of insecticides, which has led to the development of resistance to several chemical classes. In this study, the resistance levels of Myzus persicae populations collected from tomato production areas in Antalya to tau-fluvalinate, a commonly used pyrethroid, were determined. Additionally, the relationship between resistance and carboxylesterase, an important detoxification enzyme, was evaluated. The leaf-dip method was employed to determine LC50 values for the aphid populations. The resistance levels of the collected populations were calculated by comparing their LC50 values to those of a susceptible population. The tau-fluvalinate resistance ratios for the S1, S2, S3, S4, and K populations were found to be 48.8, 37.4, 35.3, 18.5, and 63.5-fold, respectively. In all field populations, carboxylesterase enzyme levels were significantly higher compared to the susceptible population. These results suggest a possible association between tau-fluvalinate resistance and carboxylesterase activity in Myzus persicae populations. Moreover, the findings indicate that regional variations in tau-fluvalinate usage frequency may contribute to differences in resistance levels.

Keywords

• resistance
• myzus persicae
• tau-fluvanite
• carboxylesterase

Myzus persicae (Hemiptera: Aphididae) Popülasyonlarında Tau-fluvalinate Direnci ve Karboksilesteraz Enzim İlişkisi

Öz

Myzus persicae birçok üründe ekonomik kayba neden olan önemli bir zararlıdır. Dünya genelinde Myzus persicae ile mücadele büyük ölçüde insektisit kullanımı tercih edilmektedir. Bu durum ise zararlının çeşitli kimyasal gruplarına karşı direnç gelişimine yol açmaktadır. Bu çalışmada, Antalya’daki domates üretim alanlarından toplanan Myzus persicae popülasyonlarında, yaygın olarak kullanılan bir piretroid olan tau-fluvalinate’a karşı direnç düzeyleri belirlenmiştir. Ayrıca, önemli bir detoksifikasyon mekanizması olan karboksilesteraz enziminin dirençle ile olan ilişkisi de değerlendirilmiştir. Yaprak biti popülasyonlarında LC50 değerlerinin belirlenmesinde yaprak daldırma metodu kullanılmıştır. Toplanan popülasyonların LC50 değerleri hassas popülasyonun LC50 değerlerine oranlanarak direnç düzeyleri belirlenmiştir. Çalışma sonucunda S1, S2, S3, S4 ve K popülasyonları için tau-fluvalinate direnç oranları sırasıyla 48.8, 37.4, 35.3, 18.5 ve 63.5 kat olarak belirlenmiştir. Tüm tarla popülasyonlarında, hassas popülasyona kıyasla karboksilesteraz enzim düzeyleri anlamlı düzeyde yüksek bulunmuştur. Sonuç olarak Myzus persicae popülasyonlarında tau-fluvalinate direnci ile karboksilesteraz enzimi arasında bir ilişki olabileceği düşünülmektedir. Aynı zamanda ve bölgede tau-fluvalinate kullanım sıklığının direnç düzeylerinde farklılıklara neden olduğunu ortaya koymaktadır.

Anahtar Kelimeler

• direnç
• karboksilesteraz
• myzus persicae
• tau-fluvanite

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