Determination of the Effectiveness of Steinernema feltiae Filipjev and Heterorhabditis bacteriophora Poinar, 1976 on Leptinotarsa decemlineata (Say, 1824)

Year 2021, Volume: 16 Issue: 2, 115 - 121, 17.12.2021

Fatma Gül Göze Özdemir , Asiye Uzun , Ozan Demirözer

Abstract

In this study, it was aimed to determine the effects of commercial entomopathogenic nematode species Steinernema feltiae and Heterorhabditis bacteriophora on the fourth larval stege and adult stages of Leptinotarsa decemlineata which cause significant economic losses in potato production areas. Three different doses of two entomopathogenic nematodes (100, 150, 200 million infective juvenile (IJs) / 100 L water) were prepared and the trials were carried out in laboratory conditions according to the randomized plot design, with 10 replications, 5 individuals for each dose. Entomopathogenic nematode suspensions were applied by spraying method under 1 atmosphere pressure for 20 seconds to individuals in both growth stages. It was determined that the application doses of both entomopathogenic nematodes in thefourth larval stage were different from the control, but there was no difference between them, and there was no difference between both control and application doses in the adult period. In addition, it was found that the mortality rates caused by S. feltiae and H. bacteriophora on the fourth larval stage of the potato beetle were between 64-82% and 20-60%, respectively. In adult individuals, these rates for entomopathogenic nematodes were between 12-24% and 2-10%, respectively.

Keywords

Biological control, Heterorhabitidae, Potato beetle, Steinernematidae

Supporting Institution

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Project Number

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Thanks

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Steinernema feltiae Filipjev ve Heterorhabditis bacteriophora Poinar, 1976’nın Leptinotarsa decemlineata (Say, 1824) Üzerindeki Etkinliğinin Belirlenmesi

Abstract

Bu çalışmada ticari entomopatojen nematod türleri olan Steinernema feltiae Filipjev ve Heterorhabditis bacteriophora Poinar, 1976 ’nın patates üretim alanlarında önemli ekonomik kayıplara neden olan Leptinotarsa decemlineata’nın dördüncü larva ve ergin dönemdeki bireyleri üzerindeki etkilerinin belirlenmesi hedeflenmiştir. İki entomopatojen nematodun 3 farklı dozu (100, 150, 200 milyon infektif juvenil (IJs)/ 100 L su) hazırlanmış ve denemeler laboratuar koşullarında tesadüf parselleri deneme desenine göre her bir doz için her tekerrürde 5 birey olmak üzere 10 tekerrürlü olarak yürütülmüştür. Entomopatojen nematod süspansiyonları patates böceğinin her iki gelişim dönemine 1 atmosfer basınç altında 20 saniye süre püskürtme yöntemi ile uygulanmıştır. Dördüncü larva döneminde her iki entomopatojen nematod türünde uygulama dozlarının kontrolden farklı ancak kendi aralarında fark olmadığı, ergin dönemde ise hem kontrol hem de uygulama dozları arasında fark olmadığı belirlenmiştir. Ayrıca, Steinernema feltiae ve H. bacteriophora'nın patates böceğinin dördüncü larva dönemi üzerinde neden oldukları ölüm oranlarının sırası ile % 64-82 ve % 20-60 arasında olduğu bulunmuştur. Ergin bireylerde ise entomopatojen nematodlar için bu oranlar sırası ile % 12-24 ve % 2-10 arasında kalmıştır.

Keywords

Biyolojik kontrol, Heterorhabitidae, Patates böceği, Steinernematidae

Project Number

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