Limon’da (Citrus limon (L.) Burm. f.) Hasat Sonrası Hastalıklara Karşı Antifungal Aktiviteye Sahip Antagonist Bakterilerin Taranması

Year 2022, Volume: 17 Issue: 2, 139 - 147, 06.12.2022

Nasibe Tekiner , Elif Tozlu , Recep Kotan

https://doi.org/10.54975/isubuzfd.1177801

Abstract

Bu çalışma, limon meyvesinde hasat sonrası kayıplara neden olan farklı fungal etmenlere karşı biyolojik mücadele etmeni olabilecek bakterileri belirlemek amacıyla in vitro koşullarda yürütülmüştür. Bu amaçla, hastalıklı limon meyvelerinden izolasyonlar yapılmış, izolasyonlar sonucu üç farklı cinse ait fungus türü izole edilmiş, patojenite testleri yapılmış ve moleküler tanı sonuçlarına göre bu izolatların Alternaria alternata (ET 119), Colletotrichum gleosporoides (ET 120) ve Penicillium chrysogenum (ET 121) olduğu tespit edilmiştir. Patojen fungus izolatlarına karşı sekiz farklı cinse ait otuz altı adet antagonist bakteri izolatının yüzde engelleme oranları ikili kültür testi ile tesadüf parselleri deneme desenine göre 3 tekerrürlü olarak belirlenmiştir. Elde edilen sonuçlara göre üç patojen izolata karşı en yüksek etki TV 53D (%67.46: Brevibacillus choshinensis) izolatında saptanırken, bu izolatı sırasıyla TV 16F (%66.27: Bacillus subtilis) ve FDG 37 (%62.30: Pseudomonas fluorescens) izolatları takip etmiştir. En etkili sonuç alınan antagonist bakteri izolatları ile gelecekte kontrollü şartlarda daha detaylı çalışmaların yürütülmesi gerekmektedir.

Keywords

Antifungal aktivite, Bacillus subtilis, antagonist bakteri, biyolojik mücadele, Pseudomonas fluorescens

Screening of Antagonist Bacteria with Antifungal Activity Against Postharvest Diseases on Lemon (Citrus limon (L.) Burm.f.)

Abstract

This study was carried out in vitro conditions to determine the bacteria biological control agents of against different fungal agents causing postharvest losses in lemon fruit. For this purpose, isolations were made from diseased lemon fruits, as a result of isolations three different fungal species were isolated, pathogenicity tests were carried out and according to the results of molecular diagnosis, these isolates were found to be Alternaria alternata (ET 119), Colletotrichum gleosporoides (ET 120) and Penicillium chrysogenum (ET 121). Percent inhibition rate of thirty-six antagonist bacterial isolates belonging to eight different genera against pathogenic fungus isolates was determined by dual culture test according to a randomized plot experiment design with 3 replications. According to the results obtained, the highest effect against the three pathogen isolates was detected in TV 53D (67.46%: Brevibacillus choshinensis), this isolate was followed by TV 16F (66.27%: Bacillus subtilis) and FDG 37 (62.30%: Pseudomonas fluorescens) isolates, respectively. More detailed studies should be carried out in the future under controlled conditions with the most effective antagonist bacterial isolates.

Keywords

Bacillus subtilis, Pseudomonas fluorescens, Antifungal activity, antagonist bacteria, biological control

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