Dentin Hassasiyet Gidericilerin Sığır Pulpasından Türetilen Hücre Canlılığı Üzerindeki Sitotoksik Etkisi

Year 2023, , 235 - 238, 15.06.2023

Mustafa Ülker , Türkay Kölüş , Esma Nur Bülbül , Hayriye Esra Ülker

https://doi.org/10.15311/selcukdentj.1232442

Abstract

Amaç: Mevcut çalışmada sığır pulpasından elde edilmiş hücreler üzerinde dentin hassasiyet giderici ajanların sitotoksisitesi değerlendirdi.
Gereç ve Yöntemler: Sitotoksisite testi için, SV40 (Simian virüsü 40) büyük T antijeni ile transfekte edilmiş sığır pulpasından elde edilmiş hücreler Shield Force Plus, Gluma ve Teethmate Desensitizer’in farklı dilüsyonlarına (orjinal, % ½ ve % ½ ) 24 saat boyunca maruz bırakıldı. Kontrol grubu olarak tam hücre kültür ortamı kullanıldı. Sığır pulpasından elde edilmiş hücrelerin canlılığı MTT testi ile belirlendi. Veriler tek yönlü varyans analizi (ANOVA) ve Tukey HSD testleri kullanılarak analiz edildi.
Bulgular: Shield Force Plus ve Teethmate Desensitizer grupları, kontrol ile karşılaştırıldığında sığır pulpasından elde edilmiş hücreler sağkalımını değiştirmedi (p>0.05). Gluma, tüm konsantrasyonlarda sığır pulpasından elde edilmiş hücreler üzerinde sitotoksik etki gösterdi (p<0.05).
Sonuçlar: Shield Force Plus ve Teethmate Desensitizer, sığır pulpasından elde edilmiş hücreler üzerine Gluma'dan daha biyouyumludur.

Keywords

hassasiyet gidericiler, sitotoksisite, MTT

Cytotoxic Effect of Dentin Desensitizers on Bovine Pulp Derived Cell Viability

Abstract

Aim: Present study evaluated the cytotoxicity of dentin desentisizing agents on bovine pulp-derived cells (bDPCs).
Materials and Methods: Transfected bDPCs cells were exposed to original, ½ and ¼ dilutions of Shield Force Plus, Gluma and Teethmate Desensitizer for 24 h. Culture medium was used as a control group. The bDPCs viability was tested by MTT after 24 hours of exposure. Data were analyzed using the one-way analysis of variance (ANOVA) and the Tukey HSD tests.
Results: Neither of the Shield Force Plus and Teethmate Desensitizers change the survival rate of bovine pulp-derived cells when compared to the control (p>0.05). Gluma Desensitizer have cytotoxic effects on bovine pulp-derived cells at all dilutions (p<0.05).
Conclusion: Shield Force Plus and Teethmate Desensitizer were more biocompatible than Gluma on bDPCs.

Keywords

desensitizers, cytotoxicity, MTT

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