Buğday (Triticum durum desf.) ve Arpa (Hordeum vulgare L.) nın Vitro Fidelerinin Bor Alımının ICP-AES ile Tespiti

Abstract

Bu arastirma, ICP-AES (Inductively Coupled Plasma-Atomic Emission Spectrometry) ile bugday (Triticum durum Desf.) ve arpa (Hordeum vulgare L.) in vitro fidelerinin bor (B) alimi ve biriktirme durumlarini belirlemek amaciyla yürütülmüstür. Tohumlar; 200 ml’lik cam kavanozlarda, %0.7 agar, %3 sakaroz ve sirasiyla; 0.0, 6.2, 18.6, 55.8, 111.6 mg/l H3BO3 içeren 50 ml MS besin ortaminda, her kavanozda 5 adet tohum olacak sekilde kültüre alinmistir. Yirmi günlük fidelerin kurutulmus kök ve gövde kisimlari (0.1-1 g) ile yetistirme ortamlari (5.0-6.0 g) mikrodalgada (CEM-Mars x 5) 10 ml HNO3 ile 170 PSI basinçta 200°C’de 40 dak. yakilmis ve numunelerde ICP-AES ile bor analizi yapilmistir. Kiziltan-91’de köklerde en az B birikimi 2.2 μg/g kuru madde olarak kontrol ortamindan elde edilirken, en çok kök B içerigi ise 15.1 μg B/g ile 111.6 mg/L H3BO3 içeren ortamdan elde edilmistir. Gövdede B birikimi en az 4.9 μg B/g ile kontrolde, en çok 67.6 μg B/g ile 111.6 mg/l H3BO3 içeren ortaminda olmustur. Tokak-157/37’de en düsük kök B konsantrasyonunun 0.53 μg B/g ile yine kontrol ortaminda, en yüksek birikimi ise 17.3 μg B/g ile 111.6 mg/l H3BO3 de olmustur. Arpada en düsük gövde B konsantrasyonunun 0.74 μg B/g ile 0 mg/l H3BO3 da, en yüksek 100.5 μg B/g ile 111.6 mg/l H3BO3 da yetistirilen fidelerin gövdelerinde biriktigi belirlenmistir.

Keywords

• Bor alimi
• MS besin ortami
• arpa
• bugday
• ICP-AES

Boron Uptake of in Vitro Seedlings of Wheat (Triticum durum Desf.) and Barley (Hordeum vulgare L.) as Determined by ICP-AES

Abstract

Boron (B) absorption of in vitro seedlings of wheat (Triticum durumDesf.) and barley (Hordeum vulgareL.) and its accumulation in various organs were investigated using an Inductively Coupled Plasma Atomic Emission Spectrometry (ICPAES). Dried plant samples (0.1-1 g) from 20-d old seedlings and plant culture media (5.0-6.0 g) were transferred into plastic bottles containing 10 ml of 65 % (v/v) nitric acid (HNO3) solution. Samples were ashed at 200°C for 40 min in a microwave (CEM-Mars x 5) followed by analyses in the ICP-AES device. The lowest concentration of B (2.2 μg B/g dry matter) accumulation was found in roots of Kiziltan-91 seedlings cultured in the control medium containing 0 mg/l B whereas the highest B accumulation (15.1 μg B/g dry matter) was detected again in roots of Kiziltan-91 in medium containing the highest concentration of B that was incorporated into the medium as H3BO3. Similarly, the highest level of B absorption by the shoots of Kiziltan-91 took place in media containing the highest level of boric acid (111.6 mg/l) with the lowest B concentration was found in shoots at the lowest medium B concentration (0 mg/l), showing that there was a linear correlation with respect to B content of the culture medium and B accumulation in the organs of Kiziltan-91. The B absorption response of barley seedlings were similar to that of wheat.

Keywords

• B absorptions
• MS medium
• barley
• wheat
• ICP-AES