Determination of Calpastatin Gene Polymorphism in Kıvırcık Crossbred Ewes by PCR-RFLP Method
Abstract
Calpastatin (CAST) is an endogenous and specific inhibitor of calpains found in meat. Because of this feature, CAST is a major gene that directly changes the toughness of meat. The objective of this study to determine the CAST gene polymorphisms in Kıvırcık crossbred ewes. Therefore, CAST gene polymorphisms were investigated in 100 Kıvırcık crossbred ewes grown in Thrace region. The PCR-RFLP method was used to determine the genetic variations of the CAST gene. In the current study, the estimated frequencies of three genotypes including MM, MN and NN at CAST/MspI polymorphism were 0.82, 0.16 and 0.02 and they were 0.90 and 0.10 for M and N alleles, respectively. There was no deviation from Hardy-Weinberg equilibrium (P>0.05) relative to CAST genotypes.
Keywords
Details
Primary Language
English
Subjects
Zootechny (Other)
Journal Section
Research Article
Publication Date
December 29, 2017
Submission Date
October 5, 2017
Acceptance Date
-
Published in Issue
Year 2017 Volume: 31 Number: 3
APA
Kaplan, S., & Atalay, S. (2017). Determination of Calpastatin Gene Polymorphism in Kıvırcık Crossbred Ewes by PCR-RFLP Method. Selcuk Journal of Agriculture and Food Sciences, 31(3), 147-150. https://izlik.org/JA32GK58PE
AMA
1.Kaplan S, Atalay S. Determination of Calpastatin Gene Polymorphism in Kıvırcık Crossbred Ewes by PCR-RFLP Method. Selcuk J Agr Food Sci. 2017;31(3):147-150. https://izlik.org/JA32GK58PE
Chicago
Kaplan, Selçuk, and Sertaç Atalay. 2017. “Determination of Calpastatin Gene Polymorphism in Kıvırcık Crossbred Ewes by PCR-RFLP Method”. Selcuk Journal of Agriculture and Food Sciences 31 (3): 147-50. https://izlik.org/JA32GK58PE.
EndNote
Kaplan S, Atalay S (December 1, 2017) Determination of Calpastatin Gene Polymorphism in Kıvırcık Crossbred Ewes by PCR-RFLP Method. Selcuk Journal of Agriculture and Food Sciences 31 3 147–150.
IEEE
[1]S. Kaplan and S. Atalay, “Determination of Calpastatin Gene Polymorphism in Kıvırcık Crossbred Ewes by PCR-RFLP Method”, Selcuk J Agr Food Sci, vol. 31, no. 3, pp. 147–150, Dec. 2017, [Online]. Available: https://izlik.org/JA32GK58PE
ISNAD
Kaplan, Selçuk - Atalay, Sertaç. “Determination of Calpastatin Gene Polymorphism in Kıvırcık Crossbred Ewes by PCR-RFLP Method”. Selcuk Journal of Agriculture and Food Sciences 31/3 (December 1, 2017): 147-150. https://izlik.org/JA32GK58PE.
JAMA
1.Kaplan S, Atalay S. Determination of Calpastatin Gene Polymorphism in Kıvırcık Crossbred Ewes by PCR-RFLP Method. Selcuk J Agr Food Sci. 2017;31:147–150.
MLA
Kaplan, Selçuk, and Sertaç Atalay. “Determination of Calpastatin Gene Polymorphism in Kıvırcık Crossbred Ewes by PCR-RFLP Method”. Selcuk Journal of Agriculture and Food Sciences, vol. 31, no. 3, Dec. 2017, pp. 147-50, https://izlik.org/JA32GK58PE.
Vancouver
1.Selçuk Kaplan, Sertaç Atalay. Determination of Calpastatin Gene Polymorphism in Kıvırcık Crossbred Ewes by PCR-RFLP Method. Selcuk J Agr Food Sci [Internet]. 2017 Dec. 1;31(3):147-50. Available from: https://izlik.org/JA32GK58PE