Determination of Calpastatin Gene Polymorphism in Kıvırcık Crossbred Ewes by PCR-RFLP Method
Öz
Calpastatin (CAST) is an endogenous and specific inhibitor of calpains found in meat. Because of this feature, CAST is a major gene that directly changes the toughness of meat. The objective of this study to determine the CAST gene polymorphisms in Kıvırcık crossbred ewes. Therefore, CAST gene polymorphisms were investigated in 100 Kıvırcık crossbred ewes grown in Thrace region. The PCR-RFLP method was used to determine the genetic variations of the CAST gene. In the current study, the estimated frequencies of three genotypes including MM, MN and NN at CAST/MspI polymorphism were 0.82, 0.16 and 0.02 and they were 0.90 and 0.10 for M and N alleles, respectively. There was no deviation from Hardy-Weinberg equilibrium (P>0.05) relative to CAST genotypes.
Anahtar Kelimeler
Ayrıntılar
Birincil Dil
İngilizce
Konular
Hayvansal Üretim (Diğer)
Bölüm
Araştırma Makalesi
Yayımlanma Tarihi
29 Aralık 2017
Gönderilme Tarihi
5 Ekim 2017
Kabul Tarihi
-
Yayımlandığı Sayı
Yıl 2017 Cilt: 31 Sayı: 3
APA
Kaplan, S., & Atalay, S. (2017). Determination of Calpastatin Gene Polymorphism in Kıvırcık Crossbred Ewes by PCR-RFLP Method. Selcuk Journal of Agriculture and Food Sciences, 31(3), 147-150. https://izlik.org/JA32GK58PE
AMA
1.Kaplan S, Atalay S. Determination of Calpastatin Gene Polymorphism in Kıvırcık Crossbred Ewes by PCR-RFLP Method. Selcuk J Agr Food Sci. 2017;31(3):147-150. https://izlik.org/JA32GK58PE
Chicago
Kaplan, Selçuk, ve Sertaç Atalay. 2017. “Determination of Calpastatin Gene Polymorphism in Kıvırcık Crossbred Ewes by PCR-RFLP Method”. Selcuk Journal of Agriculture and Food Sciences 31 (3): 147-50. https://izlik.org/JA32GK58PE.
EndNote
Kaplan S, Atalay S (01 Aralık 2017) Determination of Calpastatin Gene Polymorphism in Kıvırcık Crossbred Ewes by PCR-RFLP Method. Selcuk Journal of Agriculture and Food Sciences 31 3 147–150.
IEEE
[1]S. Kaplan ve S. Atalay, “Determination of Calpastatin Gene Polymorphism in Kıvırcık Crossbred Ewes by PCR-RFLP Method”, Selcuk J Agr Food Sci, c. 31, sy 3, ss. 147–150, Ara. 2017, [çevrimiçi]. Erişim adresi: https://izlik.org/JA32GK58PE
ISNAD
Kaplan, Selçuk - Atalay, Sertaç. “Determination of Calpastatin Gene Polymorphism in Kıvırcık Crossbred Ewes by PCR-RFLP Method”. Selcuk Journal of Agriculture and Food Sciences 31/3 (01 Aralık 2017): 147-150. https://izlik.org/JA32GK58PE.
JAMA
1.Kaplan S, Atalay S. Determination of Calpastatin Gene Polymorphism in Kıvırcık Crossbred Ewes by PCR-RFLP Method. Selcuk J Agr Food Sci. 2017;31:147–150.
MLA
Kaplan, Selçuk, ve Sertaç Atalay. “Determination of Calpastatin Gene Polymorphism in Kıvırcık Crossbred Ewes by PCR-RFLP Method”. Selcuk Journal of Agriculture and Food Sciences, c. 31, sy 3, Aralık 2017, ss. 147-50, https://izlik.org/JA32GK58PE.
Vancouver
1.Selçuk Kaplan, Sertaç Atalay. Determination of Calpastatin Gene Polymorphism in Kıvırcık Crossbred Ewes by PCR-RFLP Method. Selcuk J Agr Food Sci [Internet]. 01 Aralık 2017;31(3):147-50. Erişim adresi: https://izlik.org/JA32GK58PE