Nar Kabuğu Polar Fraksiyonlarının ve Amoksisilin Trihidratın Enterococcus faecalis Üzerindeki Sinerjistik Etkisi Hakkında bir Çalışma

Abstract

Çoklu antibiyotik direncine sahip bakterilerin ortaya çıkışındaki artış nedeniyle yeni antimikrobiyal maddelerin ve antimikrobiyal ilaç kombinasyonlarının keşfedilmesine ihtiyaç duyulmaktadır. Bu çalışmanın amacı, nar (Punica granatum L.) kabuğu polar fraksiyonları ile Amoksisilin trihidrat arasındaki sinerjistik etkileşimin Enterococcus faecalis üzerinde araştırılmasıdır. Çalışmanın ilk adımında, nar kabuğunun polar fraksiyonları (etil asetat, butanol, distile su) hazırlanmıştır. Sonraki aşamada sinerjik etkinin belirlenmesi için disk difüzyon yöntemi kullanılmıştır. Hazırlanan nar kabuğu polar fraksiyonları ile Amoksisilin trihidrat karışımının, E. faecalis üzerinde sinerjistik etkisinin olduğu gözlemlenmiştir. Bu çalışma, Amoksisilin trihidratın E. faecalis üzerindeki etkinliğini nar kabuğunun polar fraksiyonlarının (etil asetat, butanol, distile su) arttırdığını belirlemiştir. Nar kabuğu, kolay erişilebilirliği, terapötik özellikleri ve düşük maliyeti nedeniyle yeni antibakteriyal tedavi kombinasyonlarında potansiyel uygulamalara sahip olabilir. Bu çalışmanın sonuçları, antibiyotik etkinliğini arttıran fitokimyasal bileşiklerin karakterizasyonu ile ilgili daha ileri çalışmalar için yararlı olabilir.

Keywords

• Amoksisilin trihidrat
• Antibakteriyal aktivite
• Sinerji
• Nar

A Study About the Synergy of Polar Fractions of Pomegranate Peel and Amoxicillin Trihydrate Against Enterococcus faecalis

Abstract

The increase in the occurrence of the multidrug-resistant bacteria is the cause of need in the discovering new antimicrobial substances and antimicrobial combination drugs. The aim of this study was to investigate the synergistic interaction between polar fractions of pomegranate (Punica granatum L.) peel and Amoxicillin trihydrate against Enterococcus faecalis. In the first step of the study, polar fractions of pomegranate peel (ethyl acetate, butanol, distilled water) were prepared. Next, disc diffusion method was used for determination of the synergic effect. Here, the results indicated that the mixture of prepared polar fractions of pomegranate peel and Amoxicillin trihydrate showed synergistic interaction against E. faecalis. This study determined that polar fractions of pomegranate peel (ethyl acetate, butanol, distilled water) improved the effectiveness of Amoxicillin trihydrate against E. faecalis. Pomegranate peel may have potential applications in the new antibacterial therapy combinations due to its easy accessibility, therapeutic properties and being low cost. Results of the present study may be beneficial for further studies about the characterization of phytochemical compounds that are responsible for the improvement of the antibiotic effectiveness.

Keywords

• Amoxicillin trihydrate
• Antibacterial activity
• Synergy
• Pomegranate

References

1. Ahmad, I., Zahin, M., Aqil, F., Hasan, S., Khan, M.S.A., Owais, M., 2008. Bioactive compounds from Punica granatum, Curcuma longa and Zingiber officinale and their therapeutic potential, Drugs of the Future, 33(4): 329.
2. Aiyegoro, O.A., Okoh, A.I., 2009. Use of bioactive plant products in combination with standard antibiotics: Implications in antimicrobial chemotherapy, Journal of Medicinal Plants Research, 3(13): 1147-1152.
3. Vuuren, S., Viljoen, A., 2011. Plant-based antimicrobial studies–methods and approaches to study the interaction between natural products, Planta Med, 77: 1168–1182.
4. Viuda-Martos, M., Fernández-López, J., Pérez-Álvarez, J.A., 2010. Pomegranate and its many functional components as related to human health: A review, Comprehensive Reviews in Food Science and Food Safety, 9: 635-654.
5. Stover, E., Mercure, E.W., 2007. The pomegranate: A new look at the fruit of paradise, Hortscience, 42(5).
6. Ali, K., Khan, S., Khan, N., Khan, W., Rahman, I.U., Ullah, F., Ali, K., Nisar, M., 2017. Ethnobotanical and ecological study of Punica granatum in Dir district, Khyber Pakhtunkhwa, Pakistan, Regulatory Mechanisms in Biosystems, 8(4): 656–661.
7. Johanningsmeier, S.D., Harris, G.K., 2011. Pomegranate as a functional food and nutraceutical source, Annu. Rev. Food Sci. Technol., 2: 181–201.
8. Wadhawan, R., Reddy, Y., Sharma, R., Solanki, G., 2015. Punica granatum; ancient seeds for modern cure in medical and dental field: An overview, International Journal of Traditional System of Medicine, 2(1): 1-8.

9. Mirdehghan, S.H., Rahemi, M., 2007. Seasonal changes of mineral nutrients and phenolics in pomegranate (Punica granatum L.) fruit, Scientia Horticulturae, 111: 120–127.
10. Li, Y., Guo, C., Yang, J., Wei, J., Xu, J., Cheng, S., 2006. Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract, Food Chemistry, 96: 254-260.
11. Machado, T.B., Leal, I.C.R., Amaral, A.C.F., Santos, K.R.N., Silva, M.G., Kuster, R.M., 2002. Antimicrobial ellagitannin of Punica granatum fruits, J. Braz. Chem. Soc., 13(5): 606-610.
12. Rosas-Burgos, E.C., Burgos-Hernández, A., Noguera-Artiaga, L., Kačániová, M., Hernández-Garcia, F., Cárdenas-López, J.L., Carbonell-Barrachina, A.A., 2016. Antimicrobial activity of pomegranate peel extracts as affected by cultivar, J Sci Food Agric., Doi:10.1002/jsfa.7799.
13. Mohamad, T.G.M., Khalil, A.A., 2015. Effect of agriculture waste: pomegranate (Punica granatum L.) fruits peel on some important phytopathogenic fungi and control of tomato damping-off, Journal of Applied Life Sciences International, 3(3): 103-113.
14. Al-Rawahi, A.S., Edwards, G., Al-Sibani, M., Al-Thani, G., Al-Harrasi, A.S., Rahman, M.S., 2014. Phenolic constituents of pomegranate peels (Punica granatum L.) cultivated in Oman, European Journal of Medicinal Plants, 4(3): 315-331.
15. Moradi M-T, Karimi A, Alidadi S, Saedi-Marghmaleki M, Salehian M, 2015. In vitro Anti-adenovirus activity of pomegranate (Punica granatum L.) peel extract, Advanced Herbal Medicine, 1(4): 1-8.
16. Mtunzi FM, Ejidike IP, Ledwaba I, Ahmed A, Pakade VE, Klink MJ, Modise SJ, 2017. Solvent–solvent fractionations of Combretum erythrophyllum (Burch.) leave extract: Studies of their antibacterial, antifungal, antioxidant and cytotoxicity potentials, Asian Pacific Journal of Tropical Medicine, 10(7): 670-679.
17. Hoş A, Tunç K, 2019. Enhancement of antibacterial effectiveness of amoxicillin trihydrate and ampicillin-sulbactam, Eskişehir Technical University Journal of Science and Technology C- Life Sciences and Biotechnology, 8(1):92-97.
18. Semerci AB, İnceçayır D, Mammadova V, Hoş A, Tunç K, 2020. Antimicrobial activities of Allium staticiforme and Allium subhirsutum, Bangladesh J Pharmacol, 15: 19-23.
19. Malik, A., Bai, S., Seasotiya, L., Bharti, P., Dalal, S., 2014. Antibacterial and synergistic efficacy of Punica granatum ethyl acetate extract against multidrug resistant bacterial strains, American Journal of Advanced Drug Delivery, 2(4): 522-533.
20. Braga, L.C., Leite, A.A.M., Xavier, K.G.S., Takahashi, J.A., Bemquerer, M.P., Chartone-Souza, E., Nascimento, A.M.A., 2005. Synergic interaction between pomegranate extract and antibiotics against Staphylococcus aureus, Can. J. Microbiol., 51: 541–547.
21. Lakshmi, A.V., Harasreeramulu, S., Raju, D.V.V.S., 2013. Assessment of antibacterial potential of selected medicinal plants and their interactions with antibiotics on MRSA in the health care workers of Visakhapatnam hospitals, Journal of Pharmacy Research, 6: 589-592.
22. Dey, D., Debnath, S., Hazra, S., Ghosh, S., Ray, R., Hazra, B., 2012. Pomegranate pericarp extract enhances the antibacterial activity of ciprofloxacin against extended-spectrum β-lactamase (ESBL) and metallo-β-lactamase (MBL) producing Gram-negative bacilli, Food and Chemical Toxicology, 50: 4302-4309.