Nar Kabuğu Polar Fraksiyonlarının ve Amoksisilin Trihidratın Enterococcus faecalis Üzerindeki Sinerjistik Etkisi Hakkında bir Çalışma

Öz

Çoklu antibiyotik direncine sahip bakterilerin ortaya çıkışındaki artış nedeniyle yeni antimikrobiyal maddelerin ve antimikrobiyal ilaç kombinasyonlarının keşfedilmesine ihtiyaç duyulmaktadır. Bu çalışmanın amacı, nar (Punica granatum L.) kabuğu polar fraksiyonları ile Amoksisilin trihidrat arasındaki sinerjistik etkileşimin Enterococcus faecalis üzerinde araştırılmasıdır. Çalışmanın ilk adımında, nar kabuğunun polar fraksiyonları (etil asetat, butanol, distile su) hazırlanmıştır. Sonraki aşamada sinerjik etkinin belirlenmesi için disk difüzyon yöntemi kullanılmıştır. Hazırlanan nar kabuğu polar fraksiyonları ile Amoksisilin trihidrat karışımının, E. faecalis üzerinde sinerjistik etkisinin olduğu gözlemlenmiştir. Bu çalışma, Amoksisilin trihidratın E. faecalis üzerindeki etkinliğini nar kabuğunun polar fraksiyonlarının (etil asetat, butanol, distile su) arttırdığını belirlemiştir. Nar kabuğu, kolay erişilebilirliği, terapötik özellikleri ve düşük maliyeti nedeniyle yeni antibakteriyal tedavi kombinasyonlarında potansiyel uygulamalara sahip olabilir. Bu çalışmanın sonuçları, antibiyotik etkinliğini arttıran fitokimyasal bileşiklerin karakterizasyonu ile ilgili daha ileri çalışmalar için yararlı olabilir.

Anahtar Kelimeler

• Amoksisilin trihidrat
• Antibakteriyal aktivite
• Sinerji
• Nar

A Study About the Synergy of Polar Fractions of Pomegranate Peel and Amoxicillin Trihydrate Against Enterococcus faecalis

Öz

The increase in the occurrence of the multidrug-resistant bacteria is the cause of need in the discovering new antimicrobial substances and antimicrobial combination drugs. The aim of this study was to investigate the synergistic interaction between polar fractions of pomegranate (Punica granatum L.) peel and Amoxicillin trihydrate against Enterococcus faecalis. In the first step of the study, polar fractions of pomegranate peel (ethyl acetate, butanol, distilled water) were prepared. Next, disc diffusion method was used for determination of the synergic effect. Here, the results indicated that the mixture of prepared polar fractions of pomegranate peel and Amoxicillin trihydrate showed synergistic interaction against E. faecalis. This study determined that polar fractions of pomegranate peel (ethyl acetate, butanol, distilled water) improved the effectiveness of Amoxicillin trihydrate against E. faecalis. Pomegranate peel may have potential applications in the new antibacterial therapy combinations due to its easy accessibility, therapeutic properties and being low cost. Results of the present study may be beneficial for further studies about the characterization of phytochemical compounds that are responsible for the improvement of the antibiotic effectiveness.

Anahtar Kelimeler

• Amoxicillin trihydrate
• Antibacterial activity
• Synergy
• Pomegranate

Kaynakça

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