Molecular Characterization of $Micrococcus$ sp. Associated with Insects and Their Virulence Against $Galleria$ $mellonella$ (Lepidoptera: Pyralidae)

Year 2024, Volume: 9 Issue: 1, 14 - 25, 29.06.2024

Ali Sevim

https://doi.org/10.33484/sinopfbd.1344047

Abstract

So far, more than 100 species of bacteria are known to cause disease in arthropods. Entomopathogenic bacteria have been used for many years in the microbial control of insect pests due to reasons such as cheapness, ease of mass production, host specificity, safety, and persistence in the environment. In this study, six (6) bacterial strains isolated from various insect samples ($Malacosoma$ sp. (Lepidoptera: Lasiocampidae), $Ogcodocera$ sp. (Diptera: Bombyliidae) and $Orgyia$ sp. (Lepidoptera: Erebidae)) were initially identified as $Micrococcus$ sp. at morphological level. These bacterial strains were then identified by 16S rRNA sequence analysis at the molecular level. In addition, the mortality effects of these bacterial strains against $Galleria$ $mellonella$ (Lepidoptera: Pyralidae) larvae were determined. Six bacterial strains (MK-5, AS-2, AS-3, AS-4, BB-1, and BB-5) were identified at the genus level as $Micrococcus$ sp. As a result of pathogenicity tests against $G.$ $mellonella$ larvae, only the MK-5 strain caused a 70% mortality rate, while the other strains did not cause a significant mortality rate. It is thought that the obtained results could be useful in identifying symbiotic bacteria associated with insects and determining their pathogenic properties

Keywords

$Malacosoma$ sp., $Ogcodocera$ sp., $Orgyia$ sp., $Micrococcus$, 16S rRNA, virulence

Böceklerle İlişkili $Micrococcus$ sp. Türlerinin Moleküler Karakterizasyonu ve $Galleria$ $mellonella$ (Lepidoptera: Pyralidae)’ya Karşı Virülansları

Abstract

Şimdiye kadar 100’den fazla bakteri türünün eklem bacaklılarda hastalık oluşturduğu bilinmektedir. Entomopatojenik bakteriler ucuz olmaları, kitle üretimindeki kolaylık, konak spesifikliği, güvenlik ve çevrede kalıcılık gibi nedenlerden ötürü zararlı böceklerle mikrobiyal mücadelede uzun yıllardan beri kullanılmaktadır. Bu çalışmada çeşitli böcek örneklerinden ($Malacosoma$ sp. (Lepidoptera: Lasiocampidae), $Ogcodocera$ sp. (Diptera: Bombyliidae) ve $Orgyia$ sp. (Lepidoptera: Erebidae)) izole edilen altı (6) adet bakteri suşu ilk etapta morfolojik olarak $Micrococcus$ sp. olarak tanımlanmıştır. Daha sonra bu bakteri suşlarının 16S rRNA sekans analizi ile moleküler seviyede tanımlanmaları gerçekleştirilmiştir. Ayrıca bu bakteri suşlarının $Galleria$ $mellonella$ (Lepidoptera: Pyralidae) larvalarına karşı öldürücülük etkileri belirlenmiştir. Altı adet bakteri suşu da (MK-5, AS-2, AS-3, AS-4, BB-1 ve BB-5) $Micrococcus$ sp. olarak cins düzeyinde tanımlanmıştır. $G.$ $mellonella$ larvalarına karşı patojenite testleri sonucunda ise sadece MK-5 suşu %70 ölüm oranına neden olmuş diğer suşlar önemli derecede ölüm oranına neden olmamıştır. Elde edilen sonuçların böceklerle ilişkili simbiyotik bakterilerin tanımlanmasında ve patojenik özelliklerinin belirlenmesinde faydalı olacağı düşünülmektedir.

Keywords

$Malacosoma$ sp., $Ogcodocera$ sp., $Orgyia$ sp., $Micrococcus$, 16S rRNA, virülans

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