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Seseli tortuosum’un Antioksidan Aktivitesi ve Yağ Asidi Kompozisyonu

Year 2017, Volume 43, Issue 2, 175 - 188, 31.10.2017

Abstract

Birçok çalışma oksidatif stresle ilişkili çeşitli hastalıkların engellenmesinin yanı sıra insan sağlığına bitki sekonder metabolitlerinden kaynaklanan faydaları göstermiştir. Bu çalışmada Seseli tortuosum’un antioksidan aktivitesi serbest radikal süpürme (DPPH), indirgeme gücü (FRAP ve CUPRAC), toplam antioksidan kapasite (β-karoten/linoleik asit ve fosfomolibdat test) içeren farklı kimyasal metotlar tarafından araştırıldı. Ayrıca, toplam fenolik içerik Folin-Ciocalteu testi tarafından tespit edildi. BHA, BHT ve Troloks bu antioksidan testlerde standart olarak kullanıldı. S. tortuosum’un yağ asiti kompozisyonu Gaz Kromatografisi (GC) tarafından değerlendirildi. Özütte toplam fenolik içerik 36.58 mgGAE/g özüt olarak tespit edildi. Özüt antioksidan deneylerde konsantrasyona bağlı olarak antioksidan aktiviteye sahiptir. S. tortuosum yağında 24 yağ asiti tanımlandı. Yağ asitleri arasında, linoleik asit C 18:2 ω6 (%34.57) ana bileşendir. Doymamış yağ asitlerinin miktarı doymuş yağ asitlerinden daha yüksektir. Bu sonuçlardan,  S. tortuosum yeni gıda ve ilaç ürünlerinin tasarlanması için değerli bir kaynak olarak düşünülebilir.

References

  • 1. Halliwell B, J.M.C. G. Free radicals in Biology and Medicine, 3 rd ed. Oxford University Press. 1999;New York, USA:10-121. 2. Aruoma OI. Assessment of potential prooxidant and antioxidant actions. Journal of the American Oil Chemists' Society. 1996;73(12):1617-25. 3. Hou W-C, Lin R-D, Cheng K-T, Hung Y-T, Cho C-H, Chen C-H, et al. Free radical-scavenging activity of Taiwanese native plants. Phytomedicine. 2003;10(2-3):170-5. 4. Fridovich I. Superoxide radical and superoxide dismutases. Annual review of biochemistry. 1995;64(1):97-112. 5. Halliwell B. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? The lancet. 1994;344(8924):721-4. 6. Barlow S, Schlatter J. Risk assessment of carcinogens in food. Toxicology and applied pharmacology. 2010;243(2):180-90. 7. Vinson JA, Dabbagh YA, Serry MM, Jang J. Plant flavonoids, especially tea flavonols, are powerful antioxidants using an in vitro oxidation model for heart disease. Journal of Agricultural and Food Chemistry. 1995;43(11):2800-2. 8. Gonçalves MJ, Tavares AC, Cavaleiro C, Cruz MT, Lopes MC, Canhoto J, et al. Composition, antifungal activity and cytotoxicity of the essential oils of Seseli tortuosum L. and Seseli montanum subsp. peixotoanum (Samp.) M. Laínz from Portugal. Industrial Crops and Products. 2012;39:204-9. 9. Ilić MD, Jovanović VPS, Mitić VD, Jovanović OP, Mihajilov-Krstev TM, Marković MS, et al. Comparison of chemical composition and biological activities of Seseli rigidum fruit essential oils from Serbia. Open Chemistry. 2015;13(1). 10. Küpeli E, Tosun A, Yesilada E. Anti-inflammatory and antinociceptive activities of Seseli L. species (Apiaceae) growing in Turkey. Journal of ethnopharmacology. 2006;104(3):310-4. 11. Baytop T. Therapy with Plants in Turkey (Past and Present). 2nd ed Nobel Medical Book House, Istanbul. 1999. 12. Baytop T. Türkçe Bitki Adları Sözlügü, Atatürk Kültür, Dil ve Tarih Yüksek Kurumu, TDKY 3578. TTK Basimevi, Ankara. 1994. 13. Slinkard K, Singleton VL. Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture. 1977;28(1):49-55. 14. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical biochemistry. 1999;269(2):337-41. 15. Dapkevicius A, Venskutonis R, van Beek TA, Linssen JP. Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania. Journal of the Science of Food and Agriculture. 1998;77(1):140-6. 16. Sokmen A, Gulluce M, Akpulat HA, Daferera D, Tepe B, Polissiou M, et al. The in vitro antimicrobial and antioxidant activities of the essential oils and methanol extracts of endemic Thymus spathulifolius. Food control. 2004;15(8):627-34. 17. Sarikurkcu C. Antioxidant activities of solvent extracts from endemic Cyclamen mirabile Hildebr. tubers and leaves. African Journal of Biotechnology. 2011;10(5):831-9. 18. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical biochemistry. 1996;239(1):70-6. 19. Apak R, Güçlü K, Özyürek M, Esin Karademir S, Erçağ E. The cupric ion reducing antioxidant capacity and polyphenolic content of some herbal teas. International Journal of Food Sciences and Nutrition. 2006;57(5-6):292-304. 20. IUPAC. Standards methods for Analysis of oils, fats and derivatives Paquot, C. (ed.),. 6th edn, Oxford: Pergamon Press, . 1979:59-66. 21. Matejić J, Džamić A, Mihajilov-Krstev T, Ranđelović V, Krivošej Z, Marin P. Total phenolic content, flavonoid concentration, antioxidant and antimicrobial activity of methanol extracts from three Seseli L. taxa. Open Life Sciences. 2012;7(6):1116-22. 22. Jakovljević D, Vasić S, Stanković M, Čomić L, Topuzović M. In vitro biological activity of secondary metabolites from Seseli rigidum Waldst. et Kit.(Apiaceae). Acta biologica hungarica. 2015;66(4):395-405. 23. Abas F, Khatib A, Shaari K, Lajis NH. Chemical characterization and antioxidant activity of three medicinal Apiaceae species. Industrial Crops and Products. 2014;55:238-47. 24. Coruh N, Celep AS, Özgökçe F. Antioxidant properties of Prangos ferulacea (L.) Lindl., Chaerophyllum macropodum Boiss. and Heracleum persicum Desf. from Apiaceae family used as food in Eastern Anatolia and their inhibitory effects on glutathione-S-transferase. Food chemistry. 2007;100(3):1237-42. 25. Pandey MM, Vijayakumar M, Rastogi S, Rawat AK. Phenolic content and antioxidant properties of selected Indian spices of Apiaceae. Journal of herbs, spices & medicinal plants. 2012;18(3):246-56. 26. Zengin G, Ceylan R, Uysal S, Aktumsek A. Biological Activities of Three Extracts from Artedia squamata: A Study on Antioxidant and Enzyme Inhibitory Potential. Current Bioactive Compounds. 2015;11(3):152-5. 27. Zengin G, Bulut G, Mollica A, Haznedaroglu MZ, Dogan A, Aktumsek A. Bioactivities of Achillea phrygia and Bupleurum croceum based on the composition of phenolic compounds: In vitro and in silico approaches. Food and Chemical Toxicology. 2017. 28. Caprioli G, Fiorini D, Maggi F, Marangoni M, Papa F, Vittori S, et al. Ascorbic acid content, fatty acid composition and nutritional value of the neglected vegetable Alexanders (Smyrnium olusatrum L., Apiaceae). Journal of Food Composition and Analysis. 2014;35(1):30-6. 29. Saracoglu HT, Zengin G, Akin M, Aktumsek A. Evaluation of oil content and fatty acid composition of five endemic Bupleurum species growing in the Central Anatolia region of Turkey. Natural product research. 2012;26(13):1188-94. 30. Saraçoğlu HT, Zengin G, Akin M, Aktümsek A. A comparative study on the fatty acid composition of the oils from five Bupleurum species collected from Turkey. Turkish Journal of Biology. 2012;36(5):527-32.

Antioxidant Activity and Fatty Acid Composition of Seseli tortuosum

Year 2017, Volume 43, Issue 2, 175 - 188, 31.10.2017

Abstract

 Many studies have shown the benefits deriving from plants secondary metabolites to human health also for the prevention of several diseases associated to oxidative stress. In this work, the antioxidant activity of Seseli tortuosum was investigated by different chemical methods including free radical scavenging (DPPH assay), reducing power (FRAP and CUPRAC assays), total antioxidant capacity (β-caroten/linoleic acid and phosphomolybdate assays). Also, total phenolic content were detected by Folin-Ciocalteu assay. BHA, BHT and Trolox were used as standards in these antioxidant assays. The fatty acid composition of S. tortuosum was also evaluated by gas chromatography (GC). Total phenolic content in the extract was detected as 36.58 mgGAE/g extract. The extract possesses antioxidant activity in a concentration-dependent manner at the antioxidant assays. Twenty-four fatty acids were identified in the S. tortuosum oil. Among fatty acids, the main component was linoleic acid (C 18:2 ω6) (34.57%). The content in unsaturated fatty acids (UFAs) was higher than that of saturated fatty acids (SFAs). From these results, S. tortuosum could be considered as a valuable source for designing novel food and pharmaceutical products.

References

  • 1. Halliwell B, J.M.C. G. Free radicals in Biology and Medicine, 3 rd ed. Oxford University Press. 1999;New York, USA:10-121. 2. Aruoma OI. Assessment of potential prooxidant and antioxidant actions. Journal of the American Oil Chemists' Society. 1996;73(12):1617-25. 3. Hou W-C, Lin R-D, Cheng K-T, Hung Y-T, Cho C-H, Chen C-H, et al. Free radical-scavenging activity of Taiwanese native plants. Phytomedicine. 2003;10(2-3):170-5. 4. Fridovich I. Superoxide radical and superoxide dismutases. Annual review of biochemistry. 1995;64(1):97-112. 5. Halliwell B. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? The lancet. 1994;344(8924):721-4. 6. Barlow S, Schlatter J. Risk assessment of carcinogens in food. Toxicology and applied pharmacology. 2010;243(2):180-90. 7. Vinson JA, Dabbagh YA, Serry MM, Jang J. Plant flavonoids, especially tea flavonols, are powerful antioxidants using an in vitro oxidation model for heart disease. Journal of Agricultural and Food Chemistry. 1995;43(11):2800-2. 8. Gonçalves MJ, Tavares AC, Cavaleiro C, Cruz MT, Lopes MC, Canhoto J, et al. Composition, antifungal activity and cytotoxicity of the essential oils of Seseli tortuosum L. and Seseli montanum subsp. peixotoanum (Samp.) M. Laínz from Portugal. Industrial Crops and Products. 2012;39:204-9. 9. Ilić MD, Jovanović VPS, Mitić VD, Jovanović OP, Mihajilov-Krstev TM, Marković MS, et al. Comparison of chemical composition and biological activities of Seseli rigidum fruit essential oils from Serbia. Open Chemistry. 2015;13(1). 10. Küpeli E, Tosun A, Yesilada E. Anti-inflammatory and antinociceptive activities of Seseli L. species (Apiaceae) growing in Turkey. Journal of ethnopharmacology. 2006;104(3):310-4. 11. Baytop T. Therapy with Plants in Turkey (Past and Present). 2nd ed Nobel Medical Book House, Istanbul. 1999. 12. Baytop T. Türkçe Bitki Adları Sözlügü, Atatürk Kültür, Dil ve Tarih Yüksek Kurumu, TDKY 3578. TTK Basimevi, Ankara. 1994. 13. Slinkard K, Singleton VL. Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture. 1977;28(1):49-55. 14. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical biochemistry. 1999;269(2):337-41. 15. Dapkevicius A, Venskutonis R, van Beek TA, Linssen JP. Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania. Journal of the Science of Food and Agriculture. 1998;77(1):140-6. 16. Sokmen A, Gulluce M, Akpulat HA, Daferera D, Tepe B, Polissiou M, et al. The in vitro antimicrobial and antioxidant activities of the essential oils and methanol extracts of endemic Thymus spathulifolius. Food control. 2004;15(8):627-34. 17. Sarikurkcu C. Antioxidant activities of solvent extracts from endemic Cyclamen mirabile Hildebr. tubers and leaves. African Journal of Biotechnology. 2011;10(5):831-9. 18. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical biochemistry. 1996;239(1):70-6. 19. Apak R, Güçlü K, Özyürek M, Esin Karademir S, Erçağ E. The cupric ion reducing antioxidant capacity and polyphenolic content of some herbal teas. International Journal of Food Sciences and Nutrition. 2006;57(5-6):292-304. 20. IUPAC. Standards methods for Analysis of oils, fats and derivatives Paquot, C. (ed.),. 6th edn, Oxford: Pergamon Press, . 1979:59-66. 21. Matejić J, Džamić A, Mihajilov-Krstev T, Ranđelović V, Krivošej Z, Marin P. Total phenolic content, flavonoid concentration, antioxidant and antimicrobial activity of methanol extracts from three Seseli L. taxa. Open Life Sciences. 2012;7(6):1116-22. 22. Jakovljević D, Vasić S, Stanković M, Čomić L, Topuzović M. In vitro biological activity of secondary metabolites from Seseli rigidum Waldst. et Kit.(Apiaceae). Acta biologica hungarica. 2015;66(4):395-405. 23. Abas F, Khatib A, Shaari K, Lajis NH. Chemical characterization and antioxidant activity of three medicinal Apiaceae species. Industrial Crops and Products. 2014;55:238-47. 24. Coruh N, Celep AS, Özgökçe F. Antioxidant properties of Prangos ferulacea (L.) Lindl., Chaerophyllum macropodum Boiss. and Heracleum persicum Desf. from Apiaceae family used as food in Eastern Anatolia and their inhibitory effects on glutathione-S-transferase. Food chemistry. 2007;100(3):1237-42. 25. Pandey MM, Vijayakumar M, Rastogi S, Rawat AK. Phenolic content and antioxidant properties of selected Indian spices of Apiaceae. Journal of herbs, spices & medicinal plants. 2012;18(3):246-56. 26. Zengin G, Ceylan R, Uysal S, Aktumsek A. Biological Activities of Three Extracts from Artedia squamata: A Study on Antioxidant and Enzyme Inhibitory Potential. Current Bioactive Compounds. 2015;11(3):152-5. 27. Zengin G, Bulut G, Mollica A, Haznedaroglu MZ, Dogan A, Aktumsek A. Bioactivities of Achillea phrygia and Bupleurum croceum based on the composition of phenolic compounds: In vitro and in silico approaches. Food and Chemical Toxicology. 2017. 28. Caprioli G, Fiorini D, Maggi F, Marangoni M, Papa F, Vittori S, et al. Ascorbic acid content, fatty acid composition and nutritional value of the neglected vegetable Alexanders (Smyrnium olusatrum L., Apiaceae). Journal of Food Composition and Analysis. 2014;35(1):30-6. 29. Saracoglu HT, Zengin G, Akin M, Aktumsek A. Evaluation of oil content and fatty acid composition of five endemic Bupleurum species growing in the Central Anatolia region of Turkey. Natural product research. 2012;26(13):1188-94. 30. Saraçoğlu HT, Zengin G, Akin M, Aktümsek A. A comparative study on the fatty acid composition of the oils from five Bupleurum species collected from Turkey. Turkish Journal of Biology. 2012;36(5):527-32.

Details

Subjects Biology
Journal Section Articles
Authors

Sengul Uysal
Türkiye


Gokhan Zengin


Gokalp Ozmen Guler


Abdurrahman AKTUMSEK

Publication Date October 31, 2017
Published in Issue Year 2017, Volume 43, Issue 2

Cite

Bibtex @research article { sufefd310567, journal = {Selçuk Üniversitesi Fen Fakültesi Fen Dergisi}, issn = {}, eissn = {2458-9411}, address = {Selçuk Üniversitesi Fen Fakültesi Biyoloji Bölümü 3. Kat No: 324, Selçuklu, 42250, KONYA}, publisher = {Selcuk University}, year = {2017}, volume = {43}, pages = {175 - 188}, doi = {}, title = {Seseli tortuosum’un Antioksidan Aktivitesi ve Yağ Asidi Kompozisyonu}, key = {cite}, author = {Uysal, Sengul and Zengin, Gokhan and Guler, Gokalp Ozmen and Aktumsek, Abdurrahman} }
APA Uysal, S. , Zengin, G. , Guler, G. O. & Aktumsek, A. (2017). Seseli tortuosum’un Antioksidan Aktivitesi ve Yağ Asidi Kompozisyonu . Selçuk Üniversitesi Fen Fakültesi Fen Dergisi , 43 (2) , 175-188 . Retrieved from https://dergipark.org.tr/en/pub/sufefd/issue/31772/310567
MLA Uysal, S. , Zengin, G. , Guler, G. O. , Aktumsek, A. "Seseli tortuosum’un Antioksidan Aktivitesi ve Yağ Asidi Kompozisyonu" . Selçuk Üniversitesi Fen Fakültesi Fen Dergisi 43 (2017 ): 175-188 <https://dergipark.org.tr/en/pub/sufefd/issue/31772/310567>
Chicago Uysal, S. , Zengin, G. , Guler, G. O. , Aktumsek, A. "Seseli tortuosum’un Antioksidan Aktivitesi ve Yağ Asidi Kompozisyonu". Selçuk Üniversitesi Fen Fakültesi Fen Dergisi 43 (2017 ): 175-188
RIS TY - JOUR T1 - Seseli tortuosum’un Antioksidan Aktivitesi ve Yağ Asidi Kompozisyonu AU - Sengul Uysal , Gokhan Zengin , Gokalp Ozmen Guler , Abdurrahman Aktumsek Y1 - 2017 PY - 2017 N1 - DO - T2 - Selçuk Üniversitesi Fen Fakültesi Fen Dergisi JF - Journal JO - JOR SP - 175 EP - 188 VL - 43 IS - 2 SN - -2458-9411 M3 - UR - Y2 - 2017 ER -
EndNote %0 Selcuk University Journal of Science Faculty Seseli tortuosum’un Antioksidan Aktivitesi ve Yağ Asidi Kompozisyonu %A Sengul Uysal , Gokhan Zengin , Gokalp Ozmen Guler , Abdurrahman Aktumsek %T Seseli tortuosum’un Antioksidan Aktivitesi ve Yağ Asidi Kompozisyonu %D 2017 %J Selçuk Üniversitesi Fen Fakültesi Fen Dergisi %P -2458-9411 %V 43 %N 2 %R %U
ISNAD Uysal, Sengul , Zengin, Gokhan , Guler, Gokalp Ozmen , Aktumsek, Abdurrahman . "Seseli tortuosum’un Antioksidan Aktivitesi ve Yağ Asidi Kompozisyonu". Selçuk Üniversitesi Fen Fakültesi Fen Dergisi 43 / 2 (October 2017): 175-188 .
AMA Uysal S. , Zengin G. , Guler G. O. , Aktumsek A. Seseli tortuosum’un Antioksidan Aktivitesi ve Yağ Asidi Kompozisyonu. sufefd. 2017; 43(2): 175-188.
Vancouver Uysal S. , Zengin G. , Guler G. O. , Aktumsek A. Seseli tortuosum’un Antioksidan Aktivitesi ve Yağ Asidi Kompozisyonu. Selçuk Üniversitesi Fen Fakültesi Fen Dergisi. 2017; 43(2): 175-188.
IEEE S. Uysal , G. Zengin , G. O. Guler and A. Aktumsek , "Seseli tortuosum’un Antioksidan Aktivitesi ve Yağ Asidi Kompozisyonu", Selçuk Üniversitesi Fen Fakültesi Fen Dergisi, vol. 43, no. 2, pp. 175-188, Oct. 2017

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