Teratological Effects of Tribenuron Methyl on Zebra Fish Embryo and Larva

Year 2021, Volume: 47 Issue: 1, 47 - 60, 30.04.2021

Nazan Deniz Yön Ertuğ , Tarık Dinç , Burcu Öztürk

https://doi.org/10.35238/sufefd.848117

Abstract

Tribenuron methyl is an herbicide used in agriculture to control perennial dicots. It can access aquatic ecosystems through groundwater and affect aquatic life. The zebrafish (Danio rerio Hamilton, 1822) is a vertebrate model that has an easy reproductive capacity and transparent embryos therefore frequently preferred in scientific studies. In this study, the effects of 1.812 mg L-1, 0.906 mg L-1, 0.453 mg L-1, 0.226 mg L-1 and 0.1113 mg L-1 tribenuron methyl on zebrafish embryo and larval development were investigated. As a result of this study, it was determined that tribenuron methyl administration had toxic effects on embryo cells and caused different malformations.

Keywords

Tribenuron Methyl, Zebrafish (Danio rerio), Embryo Toxicity Test, Teratology

Tribenuron Metil’in Zebra Balığı Embriyo ve Larvaları Üzerine Teratolojik Etkileri

Abstract

Tribenuron metil tarımda çok yıllık dikotları kontrol etmek için kullanılan herbisittir. Sucul ekosistemlere yeraltı suyu yoluyla erişebilir ve sucul yaşamı etkileyebilir. Zebra balığı (Danio rerio Hamilton, 1822), kolay üreme kapasitesine ve şeffaf embriyolara sahip olan ve bu yüzden bilimsel çalışmalarda sıkça tercih edilen bir omurgalı modelidir. Bu çalışmada tribenuron metil zebra balıkları üzerinde 120 saatlik LC50 değerinin 1.850 mg L-1 olarak tespit edildi. Bu değerden yola çıkarak uygulanan 1.812 mg L-1, 0.906 mg L-1, 0.453 mg L-1, 0.226 mg L-1 ve 0.113 mg L-1 konsantrasyonlarında tribenuron metilin zebra balığı embriyo ve larva gelişimine olan etkisi incelenmiştir. Bu çalışma sonucunda tribenuron metil uygulamasının embriyo hücrelerinde toksik etki ettiği, farklı malformasyonlara sebep olduğu tespit edilmiştir.

Keywords

Tribenuron Metil, Zebra Balığı (Danio rerio), Embriyo Toksite Testi, Teratoloji

References

• Akbulut C, Ozturk B, Uzun A, Yon ND (2017). Tribenuron methyl exposure inhibits oogenesis in zebrafish (Danio rerio). Indian J. Fish 64: 127-131.
• Baghfalaki M, Shaluei F, Hedayati A, Jahanbakhshi A, Khalili M (2012). Acute toxicity assessment of tribenuron-methyl herbicide in silver carp (Hypophthalmicthys molitrix), common carp (Cyprinus carpio) and Caspian roach (Rutilus rutilus caspicus). Global Veterinaria 8: 280-284.
• Chen J, Saleem M, Wang C, Liang W, Zhang Q (2018). Individual and combined effects of herbicide tribenuron-methyl and fungicide tebuconazole on soil earthworm (Eisenia fetida). Scientific Reports 8: 1-9.
• Chen X, Teng M, Zhang J, Qian L, Duan M, Cheng Y, Zhao F, Zheng J, Wang C (2020). Tralopyril induces developmental toxicity in zebrafish embryo (Danio rerio) by disrupting the thyroid system and metabolism. Science of the Total Environment 746: 141860. EPA (Environmental Protection Agency) (2009). Types of Pesticides. Washington D.C., USA.
• Glaberman S, Padilla S, Baron MG (2017). Evaluating the zebrafish embryotoxicity test for pesticide hazard screening. Environmental Toxicology and Chemistry 36: 1221–1226.
• Kimmel CB, Ballard WW, Kimmel SR, Ullman B, Schilling TF (1995). Stages of embryonic development of zebrafish. Developmental Dynamics 203: 253-310.
• Liu S, Deng X, Bai L (2020). Developmental toxicity and transcriptome analysis of zebrafish (Danio rerio) embryos following exposure to chiral herbicide safener benoxacor. Science of the Total Environment 143273.
• Li K, Wu J, Jiang L, Shen L, He Z, Wei P, Lv Z, He MF (2017). Developmental toxicity of 2, 4-dichlorophenoxyacetic acid in zebrafish embryos. Chemosphere 171: 40-48.
• Marzouk A, Mossa H, Sabra F (2012). Cytogenetic effects of technical and formulated Tribenuron-methyl on rat bone-marrow cells. Journal of Pharmacology and Toxicology 7: 330-337.
• Perez GL, Vera MS, Miranda LA (2011). Effects of herbicide glyphosate and glyphosate-based formulations on aquatic ecosystems. Herbicides and Environment 16: 343-368.
• Pérez J, Domingues I, Monteiro M, Soares AM, Loureiro S (2013). Synergistic effects caused by atrazine and terbuthylazine on chlorpyrifos toxicity to early-life stages of the zebrafish (Danio rerio). Environmental Science and Pollution Research 20: 4671-4680. Rachedi K, Zermane F, Tir R, Ayache F, Duran R, Lauga B, Boulahrouf A (2018). Effect of sulfonylurea tribenuron methyl herbicide on soil actinobacteria growth and characterization of resistant strains. Brazilian Journal of Microbiology 49: 79-86.
• Toros S, Maden S (1985). Tarımsal Savaş Yöntem ve İlaçları. A.Ü. Ziraat Fakültesi Yayınları, Ankara.
• Velki M, Paolo C, Nelles J, Seiler T, Hollert H (2017). Diuron and diazinon alter the behavior of zebrafish embryos and larvae in the absence of acute toxicity. Chemosphere 180: 65-76.
• Vural N (1984) Toksikoloji. A.Ü. Basımevi, Ankara.
• Wiegand C, Krause E, Steinberg C, Pflugmacher S (2001). Toxicokinetics of atrazine in embryos of the zebrafish (Danio rerio). Ecotoxicology and Environmental Safety 49: 199-205.
• Yön ND, Öztürk B, Akbulut C (2016). Histological effects of tribenuron methyl spleen tissue of zebrafish. 2nd International Congress of Forensic Toxicology Industrial and Environmental Toxicology, Ankara-Turkey.
• Zhao F, Li H, Cao F, Chen X, Liang Y, Qui L (2019) Short-term developmental toxicity and potential mechanisms of the herbicide metamifop to zebrafish (Danio rerio) embryos. Chemosphere 236: 124590.