Yerel entomopatojen nematodların Amerikan beyaz kelebeği Hyphantria cunea (Drury) (Lepidoptera: Arctiidae) üzerindeki etkinliği

Year 2019, Volume: 10 Issue: 1, 17 - 28, 24.06.2019

Çiğdem Gözel

https://doi.org/10.31019/tbmd.523446

Cited By: 2

Abstract

Amerikan beyaz kelebeği, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae) birçok süs bitkisi ve çalının
yanı sıra fındık gibi önemli bazı tarımsal ürünlerin de zararlısıdır. Bu
çalışmada yerel entomopatojen nematodların (EPN) H. cunea’nın larvaları üzerinde meydana getirdikleri ölüm oranları
laboratuvar koşullarında araştırılmıştır. Çalışmada dört EPN türü; Steinernema affine (Bovien, 1937), S. carpocapsae (Weiser, 1955), S. feltiae (Filipjev, 1934) ve H. bacteriophora (Poinar, 1975)’ya ait
toplam 10 EPN izolatı kullanılmıştır. EPN’ler Türkiye’nin farklı illerinden
elde edilmiştir. H. cunea larvaları aktif
oldukları dönemlerde Düzce’deki fındık bahçelerinden toplanmıştır. Etkinlik
denemeleri H. cunea larvaları ile
platelerde 25 ^oC’de laboratuvar koşullarında yürütülmüştür. Tek bir H. cunea larvasına 100 IJs yoğunluğu
inokule edilmiş olup kontrol platelerine sadece aynı oranda saf su verilmiştir.
Plateler nematod inokulasyonundan 24, 48 ve 72 saat sonra kontrol edilmiş ve
ölüm oranları belirlenmiştir. EPN uygulamaları H. cunea larvalarında türe bağlı olarak %25-100 arasında ölüme
neden olmuştur. Çalışmadan elde edilen sonuçlara dayanarak, EPN’lerin H. cunea’nın biyolojik mücadelesinde
kullanılma potansiyelinin doğa çalışmaları ile belirlenmesinin faydalı olacağı
düşünülmektedir.

Keywords

fındık, Amerikan beyaz kelebeği, larva, entomopatojen nematodlar, etkinlik

The efficacy of native entomopathogenic nematodes on the fall webworm Hyphantria cunea (Drury) (Lepidoptera: Arctiidae)

Abstract

The fall webworm, Hyphantria cunea (Drury) prefers mulberry and maple leaves but it can also cause damage to apple, pear, plum, cherry, quince, walnut, hazelnut and ornamental plants. In this study, the susceptibility of H. cunea to native entomopathogenic nematodes (EPNs) was investigated under laboratory conditions. Ten isolates from four EPNs, namely Steinernema affine, S. carpocapsae, S. feltiae and H. bacteriophora, were used in the study. The EPNs were isolated from different provinces of Turkey and H. cunea larvae were collected from hazelnut orchards in Düzce. The efficacy assays were conducted on 12 well plates at 25 0C using one H. cunea larva/100 infective juveniles. The mortalities of H. cunea larvae were recorded at 24, 48 and 72 h after nematode inoculation. Depending on the nematode species, 25-100% mortality of the H. cunea larvae occurred. Based on the results of this study it would be useful to determine the potential of EPNs for the biological control of H. cunea in field studies.

Keywords

Hazelnut, fall webworm, larvae, entomopathogenic nematodes, efficacy

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