Indigenous Turkish entomopathogenic fungi as potential biological control agents of the Rose Aphid, Macrosiphum rosae L. (Hemiptera: Aphididae)

Year 2023, Volume: 14 Issue: 1, 76 - 83, 25.07.2023

Derya Baki Hilal Şule Tosun Fedai Erler

https://doi.org/10.31019/tbmd.1278477

Abstract

The rose aphid, Macrosiphum rosae L. (Hemiptera: Aphididae), is one of the important pests of roses (Rosa spp.) (Rosaceae) in many parts of the world, including Türkiye. In the present study, the pathogenic activity of five indigenous entomopathogenic fungal (EPF) isolates [three isolates of Beauveria bassiana (BbDm-1, BbKm-1, BbMp-1), one isolate of Isaria farinosa (IfGp-1) and one isolate of Purpureocillium lilacinum (PlKa-1)], were tested for their activity against nymphs and adults of M. rosae under laboratory conditions. Spore suspensions of three different concentrations (1 × 107, 1 × 108 and 1 × 109 conidia/ml) obtained from a 10-day old culture of the isolates on PDA (Potato Dextrose Agar) medium were tested by spray application against both nymphs and adults of M. rosae. The mortality rate was dose-dependent and increased with spore concentration of the isolates (P < 0.05). Of the five isolates tested, two B. bassiana isolates, BbDm-1 and BbKm-1, were the most pathogenic and caused mortalities of 100% and 83.3% in nymphs, and 96.7% and 80.0% in adults, respectively, at the highest concentration (1 x 109 conidia/ml), 7 days after treatment. Overall results suggest that these two B. bassiana isolates have the potential to be used in the control of M. rosae.

Keywords

Beauveria bassiana, Entomopathogenic fungi, Isaria farinosa, Macrosiphum rosae, Purpureocillium lilacinum.

Supporting Institution

Akdeniz Üniversitesi

Project Number

BAP FDK-2019-4859

Thanks

This study was financially supported by Akdeniz University Scientific Projects Coordination Unit (Project no.: BAP FDK-2019-4859) (Antalya, Türkiye). We would also like to thank TÜBİTAK BİDEB 2228-B Domestic Doctorate Scholarship Program.

Gül Yaprakbiti, Macrosiphum rosae L. (Hemiptera: Aphididae)’nin potansiyel biyolojik mücadele etmenleri olarak yerel entomopatojen funguslar

Abstract

Gül yaprakbiti Macrosiphum rosae L. (Hemiptera: Aphididae), Türkiye'nin yanı sıra dünyanın birçok yerinde güllerin (Rosa spp.) (Rosaceae) önemli zararlılarından biridir. Bu çalışmada 5 yerel entomopatojen fungus izolatı [3 Beauveria bassiana izolatı (BbDm-1, BbKm-1, BbMp-1), 1 Isaria farinosa izolatı (IfGp-1) ve 1 Purpureocillium lilacinum izolatı (PlKa-1)] laboratuvar koşullarında M. rosae’nin nimflerine ve erginlerine karşı test edilmiştir. İzolatların 10 günlük PDA (patates dekstroz agar) ortamında kültüründen elde edilen 3 farklı konsantrasyondaki (1x107, 1x108 ve 1x109 konidia/ml) spor solüsyonları M. rosae’nin hem nimflerine hem de erginlerine karşı püskürtülerek test edilmiştir. Sonuçlar, ölüm oranının konsantrasyona bağımlı olduğunu ve izolatların etkinliğinin artan spor konsantrasyonuyla arttığını göstermiştir (P < 0.05). Test edilen 5 izolattan, 2 B. bassiana izolatı (BbDm-1 ve BbKm-1) en yüksek konsantrasyonda (1 x 109 konidia/ml) uygulamadan 7 gün sonra sırasıyla nimflerde %100 ve %83.3 ve yetişkinlerde %96.7 ve %80.0 ölümlere neden olmuştur. Elde edilen sonuçlar, bu iki B. bassiana izolatının M. rosae’nin kontrolünde kullanılma potansiyeline sahip olduğunu göstermektedir.

Keywords

Beauveria bassiana, Entomopatojenik fungus, Isaria farinosa, Macrosiphum rosae, Purpureocillium lilacinum.

Project Number

BAP FDK-2019-4859

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